Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,707,777	C→T	V9I (GTC→ATC)	rseC ←	SoxR iron‑sulfur cluster reduction factor component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,707,777	0	C	T	100.0%	193.2 / NA	51	V9I (GTC→ATC)	rseC	SoxR iron‑sulfur cluster reduction factor component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/27);  total (24/27)

CACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGC  >  NC_000913/2707636‑2707924                                                                                                                                              |                                                                                                                                                    cacaGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAg                                                                                                                                             <  1:301991/150‑1 (MQ=255) cacaGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGc                                                                                                                                            <  1:114589/151‑1 (MQ=255)    aGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGccc                                                                                                                                          >  2:506358/1‑150 (MQ=255)            aCAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGa                                                                                                                                 <  1:88796/151‑1 (MQ=255)              aaTGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATc                                                                                                                               <  2:341838/151‑1 (MQ=255)                 ggTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCAt                                                                                                                             <  1:647007/150‑1 (MQ=255)                    aTGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTgc                                                                                                                          <  2:633224/150‑1 (MQ=255)                      gCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCt                                                                                                                       <  2:686672/151‑1 (MQ=255)                      gCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCt                                                                                                                       <  2:26267/151‑1 (MQ=255)                       cGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTg                                                                                                                      <  2:671942/151‑1 (MQ=255)                           gTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGcccc                                                                                                                  >  2:237149/1‑151 (MQ=255)                                    ccAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAat                                                                                                         >  1:455631/1‑151 (MQ=255)                                      aaGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAatat                                                                                                       <  1:432940/151‑1 (MQ=255)                                            atttaACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCg                                                                                                  <  2:309216/150‑1 (MQ=255)                                               taACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCgg                                                                                                 >  1:494187/1‑148 (MQ=255)                                                aaCACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAAt                                                                                             >  2:291798/1‑151 (MQ=255)                                                   acGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCg                                                                                          >  2:229004/1‑151 (MQ=255)                                                   acGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCg                                                                                          <  1:69137/151‑1 (MQ=255)                                                    cgcgGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATGCTCGGCAATGCGt                                                                                         <  1:493477/151‑1 (MQ=255)                                                     gcgGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGtt                                                                                        <  2:766252/151‑1 (MQ=255)                                                                aaCCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTgc                                                                             >  2:724448/1‑151 (MQ=255)                                                                aaCCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTg                                                                              <  1:77818/150‑1 (MQ=255)                                                                 aCCGGCGCGTGAAGCGCGGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTgcg                                                                            <  1:761494/151‑1 (MQ=255)                                                                  ccGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTgcgg                                                                           <  2:752401/151‑1 (MQ=255)                                                                   cGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTgcggc                                                                          <  1:493995/151‑1 (MQ=255)                                                                         gTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGt                                                                    >  1:506378/1‑151 (MQ=255)                                                                                   gctgctGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACaa                                                          <  1:381802/151‑1 (MQ=255)                                                                                     tgctgcATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATg                                                        >  1:197925/1‑151 (MQ=255)                                                                                       ctgcATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGt                                                      >  2:291221/1‑151 (MQ=255)                                                                                          cATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGAt                                                   >  2:63672/1‑151 (MQ=255)                                                                                             gAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTc                                                <  2:213919/151‑1 (MQ=255)                                                                                             gAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTc                                                >  1:234015/1‑151 (MQ=255)                                                                                              aaGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCg                                               <  2:767313/151‑1 (MQ=255)                                                                                              aaGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCg                                               >  2:544871/1‑151 (MQ=255)                                                                                                        aTCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCAc                                     >  1:328669/1‑151 (MQ=255)                                                                                                          cacaACTGACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGt                                   >  2:453966/1‑151 (MQ=255)                                                                                                                 gACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCt                              <  1:797512/149‑1 (MQ=255)                                                                                                                 gACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCt                              <  1:718079/149‑1 (MQ=255)                                                                                                                 gACCAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTg                            >  2:60537/1‑151 (MQ=255)                                                                                                                   ccAGCGCCTGCCCGTTTTGCCAGGTGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGTCGTTTGCGGAGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTa                          <  1:759709/151‑1 (MQ=255)                                                                                                                   ccAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGt                           <  2:149004/150‑1 (MQ=255)                                                                                                                    cAGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTAc                         >  2:858760/1‑151 (MQ=255)                                                                                                                     aGCGCCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACt                        >  1:37705/1‑151 (MQ=255)                                                                                                                        gcCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGAc                     >  2:531989/1‑151 (MQ=255)                                                                                                                        gcCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGAc                     >  2:10267/1‑151 (MQ=255)                                                                                                                        gcCTGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGAc                     >  1:715166/1‑151 (MQ=255)                                                                                                                           tGCCCGTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGt                  >  2:377050/1‑151 (MQ=255)                                                                                                                                gTTTTGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCTGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGc             >  2:412610/1‑151 (MQ=255)                                                                                                                                 ttttGCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGc             <  1:24554/150‑1 (MQ=255)                                                                                                                                     gCCAGGAGATGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCg        <  1:436185/151‑1 (MQ=255)                                                                                                                                           agaTGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTgcgc  >  2:346706/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                    CACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGC  >  NC_000913/2707636‑2707924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑