Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,735,653	A→G	L120P (CTT→CCT)	rluD ←	23S rRNA pseudouridine(1911,1915,1917) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,735,653	0	A	G	100.0%	194.7 / NA	52	L120P (CTT→CCT)	rluD	23S rRNA pseudouridine(1911,1915,1917) synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/28);  total (24/28)

ACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGCTTCTTCT  >  NC_000913/2735505‑2735796                                                                                                                                                     |                                                                                                                                                aCGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGc                                                                                                                                               <  2:468063/151‑1 (MQ=255) aCGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGc                                                                                                                                               <  2:257226/151‑1 (MQ=255) aCGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGc                                                                                                                                               >  2:154914/1‑151 (MQ=255)   gAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCaa                                                                                                                                             <  1:781010/151‑1 (MQ=255)     gTAATTTCACGCCGCTGCAAAGATTCGACTAAACGCGCCTGAGCCGGTACGGTTTTTGCCACAACCATCAGGCCAGTGTTGTCTTTATCCCTACGATGTACTATGCCCGTACGCGCTACCTCGGCCATGGGTGGATAGTACTGAGGCatc                                                                                                                                            >  1:371364/1‑148 (MQ=255)           tCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTc                                                                                                                                      <  1:273821/150‑1 (MQ=255)                 cGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCg                                                                                                                               >  1:631643/1‑151 (MQ=255)                  gTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGt                                                                                                                              <  1:432416/151‑1 (MQ=255)                    tGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCAtt                                                                                                                                       <  1:482406/140‑1 (MQ=255)                    tGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGc                                                                                                                            >  1:163422/1‑151 (MQ=255)                    tGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGc                                                                                                                            >  2:120470/1‑151 (MQ=255)                       aaaGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCAt                                                                                                                         >  2:793136/1‑151 (MQ=255)                        aaGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATc                                                                                                                        <  2:554694/151‑1 (MQ=255)                        aaGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATc                                                                                                                        <  2:391762/151‑1 (MQ=255)                         aGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATcc                                                                                                                       <  1:485615/151‑1 (MQ=255)                         aGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATcc                                                                                                                       <  1:566271/151‑1 (MQ=255)                          gATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCg                                                                                                                      >  2:189794/1‑151 (MQ=255)                           aTTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCgg                                                                                                                     <  1:191425/151‑1 (MQ=255)                             tCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCggg                                                                                                                    <  1:17364/150‑1 (MQ=255)                               gACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGACAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGtt                                                                                                                  >  2:525707/1‑150 (MQ=255)                                          tCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAgg                                                                                                      >  1:173999/1‑151 (MQ=255)                                           cTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAgg                                                                                                      >  2:48221/1‑150 (MQ=255)                                              aGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGt                                                                                                  >  2:364597/1‑151 (MQ=255)                                                    aaCGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGTCGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTaccacc                                                                                            >  1:515165/1‑151 (MQ=255)                                                        gTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGt                                                                                        <  2:54230/151‑1 (MQ=255)                                                          ttttGCCACAACCATCAGGCCAGTGGTGGCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTc                                                                                       <  1:160895/150‑1 (MQ=255)                                                                  caACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGttt                                                                              <  1:682259/151‑1 (MQ=255)                                                                   aaCCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGttt                                                                              >  1:234779/1‑150 (MQ=255)                                                                   aaCCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGttt                                                                              >  2:743785/1‑150 (MQ=255)                                                                     ccATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTttatt                                                                           <  2:536994/151‑1 (MQ=255)                                                                       aTCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTaa                                                                         <  1:527978/151‑1 (MQ=255)                                                                         cAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCTGTACATCGGCAATGGGTGTATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAAt                                                                        <  1:793104/150‑1 (MQ=255)                                                                               aGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGataata                                                                 <  1:214209/151‑1 (MQ=255)                                                                                  ggtgTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCGGGATGTACCACCAGGTCGCGCGGTTTATTAATGATaataat                                                               <  1:330290/150‑1 (MQ=255)                                                                                     gtCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGtct                                                           <  1:179279/151‑1 (MQ=255)                                                                                     gtCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGtct                                                           <  1:845298/151‑1 (MQ=255)                                                                                           aTCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGtcttcatct                                                     >  2:751534/1‑151 (MQ=255)                                                                                                             gCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGc                                   >  1:801038/1‑151 (MQ=255)                                                                                                             gCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTAGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGc                                   >  1:544552/1‑151 (MQ=255)                                                                                                                cGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCggg                                <  2:358003/151‑1 (MQ=255)                                                                                                                 gCACGCGGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCggg                                >  2:481288/1‑150 (MQ=255)                                                                                                                    cgcgGTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGatat                            >  1:733610/1‑151 (MQ=255)                                                                                                                       ggTACATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCt                         <  2:355137/151‑1 (MQ=255)                                                                                                                          aCATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCg                      <  2:419548/151‑1 (MQ=255)                                                                                                                          aCATCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCg                      <  2:381708/151‑1 (MQ=255)                                                                                                                             tCGGCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGtt                   >  1:531414/1‑151 (MQ=255)                                                                                                                               ggCAATGGGTGGATAATAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTc                  >  2:628988/1‑150 (MQ=255)                                                                                                                                gCAATGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCa                 >  1:773067/1‑150 (MQ=255)                                                                                                                                   aTGGGTGGATAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTGCCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAAc             <  1:702254/151‑1 (MQ=255)                                                                                                                                            tAGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGcttct     <  1:133991/150‑1 (MQ=255)                                                                                                                                             aGTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGcttcttc   >  1:608237/1‑151 (MQ=255)                                                                                                                                              gTAATGAGGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGcttcttct  >  2:559807/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                ACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGCTTCTTCT  >  NC_000913/2735505‑2735796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑