Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,740,735	A→G	L139P (CTG→CCG)	aroF ←	3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,740,735	0	A	G	100.0%	205.7 / NA	59	L139P (CTG→CCG)	aroF	3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/35);  total (24/35)

TTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACC  >  NC_000913/2740591‑2740881                                                                                                                                                 |                                                                                                                                                     ttAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTccc                                                                                                                                                 >  1:208540/1‑150 (MQ=255)  tAAAACCAACCGGCTTGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCa                                                                                                                                                <  1:635240/150‑1 (MQ=255)          accGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGGCCCCCATTTAcc                                                                                                                                        >  2:232572/1‑150 (MQ=255)            cGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTcccctttt                                                                                                                                            >  1:133899/1‑139 (MQ=255)            cGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGc                                                                                                                                     <  1:49145/151‑1 (MQ=255)              gCATGGAAAGCCCGGAGGCCATTTCACGGGGAGTTTGCGATTCCGTTGGACGAGCCCCAATTGCTGACCAGCTAAACAGACCGCCCAGGTATTGCGGGCTATTCGTATCTAACGCTTCCGTCGCCAGTGGCGGtgc                                                                                                                                                  >  1:212484/1‑134 (MQ=255)                aTGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCaa                                                                                                                                 <  2:357815/151‑1 (MQ=255)                aTGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGGCCCCTATTTACCAGCTc                                                                                                                                   >  1:648138/1‑149 (MQ=255)                 tGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCa                                                                                                                                  >  2:303243/1‑149 (MQ=255)                   gAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAagca                                                                                                                              <  1:312345/151‑1 (MQ=255)                   gAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGAGCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAagca                                                                                                                              <  1:756376/151‑1 (MQ=255)                           ggAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTa                                                                                                                      <  2:109750/151‑1 (MQ=255)                              ggCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTAcgc                                                                                                                   <  2:425061/151‑1 (MQ=255)                               gCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTTACCACCCCAAGCAGCAAATTAcgc                                                                                                                   >  2:507039/1‑150 (MQ=255)                                   tttCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCCACCAGCAAATTTtgcgcggt                                                                                                              >  1:790275/1‑149 (MQ=255)                                      cACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCt                                                                                                            <  2:817781/150‑1 (MQ=255)                                         ggTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCCTATTTACCAGCTCAAGCAGCCATTTTCGCGCGGTCTGc                                                                                                          >  2:227438/1‑149 (MQ=255)                                                  gCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCtt                                                                                                <  1:816745/150‑1 (MQ=255)                                                    gATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCt                                                                                              <  2:388442/150‑1 (MQ=255)                                                        ccGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATc                                                                                         <  1:125646/151‑1 (MQ=255)                                                         cGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTACCCTATTTACCCGCTCAAACCGCAAATTAACGGCCGTCTGCAACCCGGCTCCTACa                                                                                           >  2:648325/1‑148 (MQ=255)                                                          gTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCCCATTTACCCGCCCAAGCAGCAATTTTCCCGCGGACTGCAGCCCCGcct                                                                                                >  1:706983/1‑140 (MQ=255)                                                            tGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCaaa                                                                                      <  1:662545/150‑1 (MQ=255)                                                            tGTACGAGCACCAATGGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTAATCGGATCTAACGCTTCCGTCGCCAGTGGCGTGCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCaa                                                                                       <  1:549439/149‑1 (MQ=255)                                                                 gAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGcc                                                                                <  1:380673/151‑1 (MQ=255)                                                                   gCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGccat                                                                              <  2:35809/151‑1 (MQ=255)                                                                         aTTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATCCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAATAGCCATCCat‑at                                                                       <  1:39938/151‑1 (MQ=255)                                                                            gCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATggg                                                                    <  1:344038/151‑1 (MQ=255)                                                                               gACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGa                                                                  <  2:243133/150‑1 (MQ=255)                                                                                  cAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCCTATTTACCCGCTCAAACCGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCCAAAAAGCCATCCAT‑ATGGGGGTc                                                                >  2:777637/1‑149 (MQ=255)                                                                                  cAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCCTATTTACCCGCCCAAACAGCAACTTACGCGCGATCTGCCGCCCGGCTTCTTCATCCAAAAAGCCACCCATAATggggggg                                                                >  1:312916/1‑147 (MQ=255)                                                                                  cAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGACCCCCATTCACCAGACCCAAAAGCAATTTTAGCGGGGTCTGCCGCCCGGCTTCTTCATCAAAAAAACCATCCAT‑CAgggggggg                                                               >  1:23689/1‑146 (MQ=255)                                                                                   aGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGtt                                                             <  2:634248/151‑1 (MQ=255)                                                                                      tAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGGCCCCTATTTACCCACTCCAACCGACCTTTTCCCGCCAACTGCAGGCCGGGGTTTACATCCAAAGAGACCACCAA‑ATggggggccttta                                                           >  1:790619/1‑142 (MQ=255)                                                                                        aaCAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGttaatt‑a                                                       <  1:57810/151‑1 (MQ=255)                                                                                          cAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AAc                                                     <  2:41289/151‑1 (MQ=255)                                                                                           aGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCCTATTTACCAGCTCAAGCAGCAAATTTCGCGCGATTTGCAGCCCGGCTTCTACCTCAAAAAAGCCATCCAT‑ATGGGGGTCGtttattta                                                       >  2:694605/1‑147 (MQ=255)                                                                                            gATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AAccc                                                   <  1:507003/151‑1 (MQ=255)                                                                                                   cAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAg                                            <  2:703093/151‑1 (MQ=255)                                                                                                   cAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAg                                            <  2:429300/151‑1 (MQ=255)                                                                                                      gTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCg                                         <  2:538311/151‑1 (MQ=255)                                                                                                           gCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCCTAATCCCCCGCTCCAGGCGCCAATTTCCCGCGGTCTTCAGCCCGGGTTCTACATCCAAAAAGCCCTTCAT‑ATTGGGGTCGTTTATT‑AAACCTTTTCCGCCGa                                        >  2:262574/1‑147 (MQ=255)                                                                                                           gCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAg                                     <  1:369209/150‑1 (MQ=255)                                                                                                           gCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGt                                    <  1:302337/151‑1 (MQ=255)                                                                                                              ggCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTGGt                                 <  2:45915/151‑1 (MQ=255)                                                                                                               gCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTGGTa                                <  1:558988/151‑1 (MQ=255)                                                                                                                  aTTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTgg                                  <  1:71816/146‑1 (MQ=255)                                                                                                                  aTTCGGATCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCCCCAGCAGCAATTTACGCGCGGTCTGCAGCCCCGCCTCTACATCAAAAAAGCCCTCCAT‑ATGGGGGTCGGTAATT‑AAACCTTTCCCGCCGACAgggg                                  >  2:71839/1‑146 (MQ=255)                                                                                                                        aTCTAACGCTTCCGTCGCCAGTGGCGGTTCCATATTCACCAGATCAAACAGCAATTTACGCCCGGGCTGCAGCCCGGCTTCTACATCAAAAAAGACATCCAT‑ATGGGGGTCGTTAATT‑AACCCTTTCCAGCCGACAGGGGGACGGGGgttt                       >  1:274291/1‑151 (MQ=255)                                                                                                                        aTCTAACGCTTCCGTCGCCAGTGGCGGTCCCATATTTACCAGCCCAAGCAGCAATTTTCGCGCGATCTTCAGCCCCGCTTCTACATCCAAAAAGCCATCCCT‑ATGGGGGTCGTTAATT‑TACCCTTTCCCGCCCCCCGTGGGACGGGGgttt                       >  2:669346/1‑151 (MQ=255)                                                                                                                        aTCTAACGCTTCCGTCGCCAGTGGCGGCCCCCTATTTACCAGCTCAAACAGCAATTTTCGCGCGATCTGCAGCCCGGCTTCTTCATCAAAAAAACCCTCCAT‑ATGGGGGGCGTTAATT‑AAACCCTTCCAGCCCACAGGGGGACGGGGgttt                       >  1:404970/1‑151 (MQ=255)                                                                                                                         tCTAACGCTTCCGTCGCCAGTGGCGGTCCCCTATTTACCCGCTCAAGCAGCAATTTACGCGCGGTCTGCAGCCCGGCTTCTACATCCAAAAAGCCATCCAT‑ATGGGGGTCGTTAATT‑AACCCTTTCCAGCCGACAGAGGGACGGGGgtttt                      >  2:143491/1‑151 (MQ=255)                                                                                                                           tAACGCTTCCGTCGCCAGTGGCGGTCCCATATTTACCCGCTCAAGCAGCAAATTTCGCGCGATCTGCCGCCCCGCCTCTACATCAAAAAAGCCCTCCCT‑ATGGGGGTCGTTAATTAAACCCTTTCC‑GCCGACAGAGGTACGGGGgtttt                      >  2:401065/1‑149 (MQ=255)                                                                                                                             aCGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCaaa                  <  2:545800/151‑1 (MQ=255)                                                                                                                              cGCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCaaaa                 <  1:256827/151‑1 (MQ=255)                                                                                                                               gCTTCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAAt                <  2:745464/151‑1 (MQ=255)                                                                                                                                  tCCGTCGCCAGTGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATa               <  2:841772/149‑1 (MQ=255)                                                                                                                                             tGGCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTAcc  >  1:772897/1‑151 (MQ=255)                                                                                                                                              ggCGGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTAcc  <  2:13355/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     TTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATTCACCAGCTCAAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCAT‑ATGGGGATCGTTAATT‑AACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACC  >  NC_000913/2740591‑2740881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑