Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 358,347 G→A A115V (GCG→GTG)  cynR ← transcriptional activator of cyn operon; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913358,3470GA96.2% 181.8 / ‑5.7 52A115V (GCG→GTG) cynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (25/25);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACT  >  NC_000913/358202‑358494
                                                                                                                                                 |                                                                                                                                                   
ttCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACAt                                                                                                                                                  >  1:182950/1‑149 (MQ=255)
    gTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAAt                                                                                                                                            >  1:421747/1‑151 (MQ=255)
          aaaGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGcc                                                                                                                                       <  1:272254/150‑1 (MQ=255)
          aaaGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCa                                                                                                                                      <  2:810786/151‑1 (MQ=255)
           aaGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCAt                                                                                                                                     >  2:663453/1‑151 (MQ=255)
           aaGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCAt                                                                                                                                     >  1:45643/1‑151 (MQ=255)
               aaTTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAg                                                                                                                                 <  2:105993/151‑1 (MQ=255)
                    ccTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGcc                                                                                                                            >  2:484184/1‑151 (MQ=255)
                       ccAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCg                                                                                                                           <  2:619874/149‑1 (MQ=255)
                       ccAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGAt                                                                                                                         <  2:791221/151‑1 (MQ=255)
                         aGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATaa                                                                                                                       <  2:24585/151‑1 (MQ=255)
                         aGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGACCGATa                                                                                                                        >  2:154262/1‑150 (MQ=255)
                          gCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATaa                                                                                                                       >  2:815685/1‑150 (MQ=255)
                             ccGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTa                                                                                                                   <  2:204920/151‑1 (MQ=255)
                             ccGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGACCGATAAAg                                                                                                                     <  1:154239/149‑1 (MQ=255)
                               ggCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGc                                                                                                                 >  2:413696/1‑151 (MQ=255)
                                  gAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCg                                                                                                              <  2:189024/151‑1 (MQ=255)
                                   aaTGCACAGGCGTGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTc                                                                                                               <  1:798457/149‑1 (MQ=255)
                                       cacaGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAg                                                                                                         <  1:281617/151‑1 (MQ=255)
                                             cgcgAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAACGGTgggg                                                                                                   >  1:454268/1‑151 (MQ=255)
                                                gAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTg                                                                                                <  2:559891/151‑1 (MQ=255)
                                                  aGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGAc                                                                                              <  2:38430/151‑1 (MQ=255)
                                                   ggCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACg                                                                                             <  1:770072/151‑1 (MQ=255)
                                                        tCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGAt                                                                                        >  2:341726/1‑151 (MQ=255)
                                                          ccAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGGGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATAc                                                                                      <  1:775734/151‑1 (MQ=255)
                                                             aCGTCCAACTCGTCGCCGCAAAGAATATCCTCGTTTTTCCCCTGCGACATTTCCTGTAGCTGGGGCGTGATGCTGGAATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGc                                                                                    <  1:639614/150‑1 (MQ=255)
                                                               gTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGc                                                                                 >  1:650297/1‑151 (MQ=255)
                                                                tCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGc                                                                                 <  1:420436/150‑1 (MQ=255)
                                                                tCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCg                                                                                >  1:114800/1‑151 (MQ=255)
                                                                 ccAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCg                                                                                <  1:382458/150‑1 (MQ=255)
                                                                  cAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGAt                                                                              >  2:504895/1‑151 (MQ=255)
                                                                  cAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGAt                                                                              >  1:285727/1‑151 (MQ=255)
                                                                        gtcgCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTcgc                                                                        <  1:102084/151‑1 (MQ=255)
                                                                          cgcgGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGt                                                                      >  2:62042/1‑151 (MQ=255)
                                                                              gCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTc                                                                     >  2:174745/1‑148 (MQ=255)
                                                                               cAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGTGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTc                                                                     >  1:500277/1‑147 (MQ=255)
                                                                                  aGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTc                                                                     >  1:309211/1‑144 (MQ=255)
                                                                                     atatCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCa                                                            >  1:258965/1‑150 (MQ=255)
                                                                                       atCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAc                                                          >  1:741060/1‑150 (MQ=255)
                                                                                        tCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAcat                                                        >  2:434128/1‑151 (MQ=255)
                                                                                                     ccTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGcc                                             <  1:484148/149‑1 (MQ=255)
                                                                                                         cGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGTAACATCATGAATCGCCCGttt                                        >  1:213153/1‑150 (MQ=255)
                                                                                                            cATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTAccc                                    <  2:790054/151‑1 (MQ=255)
                                                                                                             aTTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCg                                   <  2:461322/151‑1 (MQ=255)
                                                                                                                    gTAGCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCa                             <  1:413597/150‑1 (MQ=255)
                                                                                                                       gCTGGAGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTc                         >  2:22558/1‑151 (MQ=255)
                                                                                                                          gggggttGATGCTGGGATAGCGCACATAAAAATCCGCCATTAAGGGGCCGATAAAGTGGCTCGTAAAGGTGGGGGTGACGGCGATCCGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCTTTTACCCGcccc                               <  2:792361/136‑1 (MQ=255)
                                                                                                                            aGCGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTa                    <  2:490919/151‑1 (MQ=255)
                                                                                                                              cGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAcatcat                                                     >  2:144861/1‑116 (MQ=255)
                                                                                                                              cGTGATGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAcatcat                                                     <  1:144838/116‑1 (MQ=255)
                                                                                                                                  aTGCTGGGATAGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGcc               <  1:389217/150‑1 (MQ=255)
                                                                                                                                            aGCGCACATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCg      >  2:706653/1‑149 (MQ=255)
                                                                                                                                              cgcacATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACt  >  2:212687/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACT  >  NC_000913/358202‑358494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑