Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,747,358 T→C K146K (AAA→AAG ffh ← Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,747,3580TC98.4% 228.4 / ‑4.3 62K146K (AAA→AAGffhSignal Recognition Particle (SRP) component with 4.5S RNA (ffs)
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (29/32);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

AGACGACCAGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGTTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGG  >  NC_000913/2747212‑2747501
                                                                                                                                                  |                                                                                                                                               
aGACGACCAGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGa                                                                                                                                             <  2:775217/151‑1 (MQ=255)
    gacCAGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTgc                                                                                                                                         <  1:655404/151‑1 (MQ=255)
    gacCAGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTgc                                                                                                                                         >  1:417066/1‑151 (MQ=255)
      ccAGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTgccg                                                                                                                                       <  1:495401/151‑1 (MQ=255)
       cAGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTgccgc                                                                                                                                      >  2:391715/1‑151 (MQ=255)
        aGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTgccgcc                                                                                                                                     >  2:276968/1‑151 (MQ=255)
         gCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGccgccg                                                                                                                                    <  2:420533/151‑1 (MQ=255)
          cGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCgg                                                                                                                                   <  1:438451/151‑1 (MQ=255)
            gTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGc                                                                                                                                 <  1:827465/151‑1 (MQ=255)
                      gcagcaCGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTc                                                                                                                       <  1:276943/151‑1 (MQ=255)
                       cagcaCGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCg                                                                                                                      <  1:622153/151‑1 (MQ=255)
                         gcaCGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACCTCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGc                                                                                                                    >  1:787247/1‑151 (MQ=255)
                           aCGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAg                                                                                                                  <  2:313477/151‑1 (MQ=255)
                            cgtcgtAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGc                                                                                                                    >  2:88566/1‑148 (MQ=255)
                             gtcgtAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGaa                                                                                                                <  1:17403/151‑1 (MQ=255)
                             gtcgtAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGa                                                                                                                 >  1:481865/1‑150 (MQ=255)
                                 tAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAacc                                                                                                             >  1:584110/1‑150 (MQ=255)
                                     aTTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAaccacc                                                                                                          >  2:624298/1‑149 (MQ=255)
                                        tCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCa                                                                                                      <  2:47283/150‑1 (MQ=255)
                                         cAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACt                                                                                                    >  1:370950/1‑151 (MQ=255)
                                          aGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACtt                                                                                                   >  1:748504/1‑151 (MQ=255)
                                             ttGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTttct                                                                                                >  1:833085/1‑151 (MQ=255)
                                               ggCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTttcttc                                                                                              >  2:9233/1‑151 (MQ=255)
                                                    cttTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTtgtg                                                                                         >  2:778305/1‑151 (MQ=255)
                                                      ttCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTtgtg                                                                                         <  2:807580/149‑1 (MQ=255)
                                                      ttCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTtgt                                                                                          <  2:334411/148‑1 (MQ=255)
                                                        cAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCtt                                                                                      <  2:510366/150‑1 (MQ=255)
                                                        cAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTtc                                                                                     >  1:30155/1‑151 (MQ=255)
                                                            gccgcGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTcgc                                                                                 >  1:145796/1‑151 (MQ=255)
                                                             ccgcGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTcgcg                                                                                >  1:406952/1‑151 (MQ=255)
                                                                cgTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAg                                                                             <  2:758801/151‑1 (MQ=255)
                                                                 gTTAACGATATCTACCGGCTTCTGACCAACATCAGGAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCa                                                                              >  2:719109/1‑149 (MQ=255)
                                                                 gTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAgg                                                                            >  1:834505/1‑151 (MQ=255)
                                                                 gTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAgg                                                                            <  1:337229/151‑1 (MQ=255)
                                                                 gTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAgg                                                                            <  1:756196/151‑1 (MQ=255)
                                                                     aCGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACt                                                                        >  1:399421/1‑151 (MQ=255)
                                                                      cGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGaa                                                                          <  1:723481/148‑1 (MQ=255)
                                                                           tCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCg                                                                   >  1:77624/1‑150 (MQ=255)
                                                                              aCCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGtt                                                               <  1:391683/151‑1 (MQ=255)
                                                                                 ggCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTAc                                                            >  1:824990/1‑151 (MQ=255)
                                                                                        gACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCt                                                     >  2:402469/1‑151 (MQ=255)
                                                                                           cAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGgt                                                  <  2:240248/151‑1 (MQ=255)
                                                                                           cAACATCAGAAGGGAAGAAAGCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGAAACTTACCGAGTTTACCAACGCTGgt                                                  <  1:454441/151‑1 (MQ=255)
                                                                                              cATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGgttgt                                               <  1:535617/151‑1 (MQ=255)
                                                                                              cATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGgttgt                                               >  2:478904/1‑151 (MQ=255)
                                                                                                tCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGttt                                             >  1:551797/1‑151 (MQ=255)
                                                                                                  aGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGtttt                                            >  1:843471/1‑150 (MQ=255)
                                                                                                    aaGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTAcc                                         <  1:572834/151‑1 (MQ=255)
                                                                                                     aGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCg                                        <  2:698642/151‑1 (MQ=255)
                                                                                                        gaagaaATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCa                                     <  2:4058/151‑1 (MQ=255)
                                                                                                             aaTCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTg                                <  1:475708/151‑1 (MQ=255)
                                                                                                               tCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGc                               >  2:139158/1‑150 (MQ=255)
                                                                                                                                 gCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTa               <  2:758690/148‑1 (MQ=255)
                                                                                                                                 gCCAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACg             <  2:821756/150‑1 (MQ=255)
                                                                                                                                  ccAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGAc           <  2:839163/151‑1 (MQ=255)
                                                                                                                                   cAGCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGAcc          <  2:469643/151‑1 (MQ=255)
                                                                                                                                     gCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGc        >  2:514162/1‑151 (MQ=255)
                                                                                                                                     gCGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGc        >  1:530231/1‑151 (MQ=255)
                                                                                                                                      cGTCTCAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCa       <  2:145819/151‑1 (MQ=255)
                                                                                                                                           cAAGCTGTTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCgg  >  2:671901/1‑151 (MQ=255)
                                                                                                                                           cAAGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCgg  <  1:277555/151‑1 (MQ=255)
                                                                                                                                            aaGCTGCTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCgg  >  1:5322/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AGACGACCAGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTGACCAACATCAGAAGGGAAGAAATCAACGCCCACCTGCTCTGCCAGCGTCTCAAGCTGTTTGATTGCCGCCGGGCGATAAACGTCGGCAGAAACCACCAGCACTTTCTTCTTGTGCTTCTCGCGCAGGAACTTACCGAGTTTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGG  >  NC_000913/2747212‑2747501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: