Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,813,385 A→G N257S (AAC→AGC)  emrB → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,813,3850AG97.1% 101.0 / ‑3.6 34N257S (AAC→AGC) emrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (17/16);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

TGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACG  >  NC_000913/2813241‑2813521
                                                                                                                                                |                                                                                                                                        
tgGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAAccc                                                                                                                                       >  2:735020/1‑148 (MQ=255)
 ggTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGAt                                                                                                                                    >  2:693238/1‑150 (MQ=255)
 ggTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATa                                                                                                                                   <  2:407524/151‑1 (MQ=255)
   ttATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATa                                                                                                                                   >  2:482004/1‑149 (MQ=255)
   ttATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGt                                                                                                                                 >  2:120691/1‑151 (MQ=255)
       tGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGa                                                                                                                              <  1:473842/150‑1 (MQ=255)
        ggTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGAt                                                                                                                             <  2:807069/150‑1 (MQ=255)
         gTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCt                                                                                                                           <  2:92872/151‑1 (MQ=255)
         gTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCt                                                                                                                           >  2:837641/1‑151 (MQ=255)
             cGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCg                                                                                                                       <  1:538806/151‑1 (MQ=255)
             cGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCg                                                                                                                       >  2:266094/1‑151 (MQ=255)
                        aGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTc                                                                                                            <  2:559079/151‑1 (MQ=255)
                            tATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTcgcg                                                                                                         <  1:617861/150‑1 (MQ=255)
                                 tCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACtt                                                                                                   >  2:495108/1‑151 (MQ=255)
                                  cGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTc                                                                                                  <  1:745908/151‑1 (MQ=255)
                                        cgGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATc                                                                                            <  2:265126/151‑1 (MQ=255)
                                         ggTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGAGCAACTTCACCATCg                                                                                           >  2:121149/1‑151 (MQ=255)
                                            aaaGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGgc                                                                                         >  2:370621/1‑150 (MQ=255)
                                                    ggACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTtgt                                                                                  <  1:638456/149‑1 (MQ=255)
                                                    ggACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTtgt                                                                                  <  1:209768/149‑1 (MQ=255)
                                                    ggACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTgtgt                                                                                <  1:438114/151‑1 (MQ=255)
                                                      aCTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTa                                                                               >  2:104495/1‑150 (MQ=255)
                                                         ggTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCa                                                                            <  1:578296/150‑1 (MQ=255)
                                                            tttCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCt                                                                        >  2:681197/1‑151 (MQ=255)
                                                             ttCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCCATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTc                                                                       >  2:236121/1‑151 (MQ=255)
                                                               catcaCAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTcgc                                                                     >  2:239736/1‑151 (MQ=255)
                                                               catcaCAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTcg                                                                      <  2:125019/150‑1 (MQ=255)
                                                                    caGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGtat                                                                 >  1:377359/1‑150 (MQ=255)
                                                                              tcatcCTTACCGTGGTGGCGGGGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACtt                                                      >  1:450086/1‑151 (MQ=255)
                                                                                        cGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTAtt                                            <  2:110513/151‑1 (MQ=255)
                                                                                         gtggtgGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTAtt                                            >  2:620292/1‑150 (MQ=255)
                                                                                                 ggtggtGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTctgctg                                   >  2:569633/1‑151 (MQ=255)
                                                                                                                                gAGCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTac     >  1:468301/1‑150 (MQ=255)
                                                                                                                                  gCTGACCGACGATAGCCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACg  <  2:231516/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                        
TGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACG  >  NC_000913/2813241‑2813521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: