Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,817,743 T→C intergenic (‑240/+41) yqaB ← / ← argQ fructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,817,7430TC98.7% 265.1 / NA 79intergenic (‑240/+41)yqaB/argQfructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (35/43);  total (35/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATA  >  NC_000913/2817596‑2817888
                                                                                                                                                   |                                                                                                                                                 
gATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGACATTTTTTGGAGAAGAGGGTGCATCAGGGAGGCTGACTCCCTGACTTTCGCCCTCCGGGCCGTTCCTGGCGCAACGTAATCCTTCATGGTGCAGGCGGTCAATACCGCCAtc                                                                                                                                                <  1:339875/151‑1 (MQ=255)
gATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCAtc                                                                                                                                                >  2:167944/1‑151 (MQ=255)
 aTAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCAtct                                                                                                                                               >  1:554179/1‑151 (MQ=255)
   aaaGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCAtctc                                                                                                                                              <  2:310023/150‑1 (MQ=255)
   aaaGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATctct                                                                                                                                             <  2:190879/151‑1 (MQ=255)
   aaaGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATctct                                                                                                                                             <  2:199412/151‑1 (MQ=255)
   aaaGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATctct                                                                                                                                             >  1:62689/1‑151 (MQ=255)
    aaGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCtt                                                                                                                                            <  2:457583/151‑1 (MQ=255)
     aGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTc                                                                                                                                           >  2:582480/1‑151 (MQ=255)
     aGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTc                                                                                                                                           >  2:292895/1‑151 (MQ=255)
     aGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCGTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTc                                                                                                                                           <  1:390620/151‑1 (MQ=255)
       gAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCg                                                                                                                                          >  1:54515/1‑150 (MQ=255)
       gAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGa                                                                                                                                         <  2:841429/151‑1 (MQ=255)
           tATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATAcc                                                                                                                                     >  1:295293/1‑151 (MQ=255)
           tATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATAcc                                                                                                                                     >  1:722872/1‑151 (MQ=255)
           tATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATAc                                                                                                                                      >  2:773693/1‑150 (MQ=255)
            aTTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATAcc                                                                                                                                     <  1:320387/150‑1 (MQ=255)
              ttAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCtt                                                                                                                                   <  1:89765/150‑1 (MQ=255)
                aGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCAt                                                                                                                                >  1:357190/1‑151 (MQ=255)
                  aaaTGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTg                                                                                                                              >  2:409897/1‑151 (MQ=255)
                  aaaTGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTg                                                                                                                              >  1:51463/1‑151 (MQ=255)
                     tGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTg                                                                                                                           >  2:708451/1‑151 (MQ=255)
                     tGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACCTTCGCCCTTTGGGCCGTTGCTGTAACAACGTTATCCTTCCTGTTGCCTGCGGTTAATACCGCCACCCCTTCGTTACATTCTTCGCTg                                                                                                                           >  2:113303/1‑151 (MQ=21)
                      gagaTATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTg                                                                                                                           <  1:226070/150‑1 (MQ=255)
                       agaTATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGa                                                                                                                          >  2:98997/1‑150 (MQ=255)
                        gaTATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAg                                                                                                                        <  1:179095/151‑1 (MQ=255)
                             ttttGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTAc                                                                                                                   >  1:703973/1‑151 (MQ=255)
                              tttGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGtt                                                                                                                     >  1:178716/1‑148 (MQ=255)
                               ttGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACg                                                                                                                  <  1:91654/150‑1 (MQ=255)
                                     gAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAata                                                                                                           <  2:322554/151‑1 (MQ=255)
                                       aaTTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATg                                                                                                         <  1:311432/151‑1 (MQ=255)
                                         ttttttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGt                                                                                                       >  1:346493/1‑151 (MQ=255)
                                          tttttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGt                                                                                                       <  2:464442/150‑1 (MQ=255)
                                          tttttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTg                                                                                                      >  1:773557/1‑151 (MQ=255)
                                           ttttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGc                                                                                                     >  1:747077/1‑151 (MQ=255)
                                            tttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGc                                                                                                     <  2:578767/150‑1 (MQ=255)
                                             ttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCAt                                                                                                   >  1:396668/1‑151 (MQ=255)
                                              tGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCAt                                                                                                   <  2:686812/150‑1 (MQ=255)
                                                  gaggaTGGGGAATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACATTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATTCggg                                                                                              <  1:713747/148‑1 (MQ=21)
                                                   aagaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCgg                                                                                               >  1:440324/1‑149 (MQ=255)
                                                      aTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGggagga                                                                                          <  2:792703/151‑1 (MQ=255)
                                                      aTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGggagg                                                                                           >  2:729231/1‑150 (MQ=255)
                                                        ggTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGAt                                                                                         <  1:673208/150‑1 (MQ=255)
                                                           gCATCCGGGAGTATGTCTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGa                                                                                     <  1:494959/150‑1 (MQ=11)
                                                           gCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCg                                                                                      <  1:565876/150‑1 (MQ=255)
                                                                          aCTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCCGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCg                                                                      >  2:42229/1‑151 (MQ=255)
                                                                             cccTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCa                                                                                                    <  1:488758/116‑1 (MQ=255)
                                                                             cACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCa                                                                                                    >  2:488794/1‑118 (MQ=255)
                                                                             cACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                                     <  1:779395/149‑1 (MQ=255)
                                                                                 gACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                               <  1:461611/151‑1 (MQ=255)
                                                                                       cGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAg                                                         <  2:457647/151‑1 (MQ=255)
                                                                                       cGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCCCTCGGTTCGTAGACggg                                                         >  1:854988/1‑149 (MQ=255)
                                                                                          ccTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc                                                      >  2:616923/1‑151 (MQ=255)
                                                                                              cGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActct                                                   <  1:121723/150‑1 (MQ=255)
                                                                                               gggCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                                 >  2:415999/1‑151 (MQ=255)
                                                                                               gggCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                                 <  2:19322/151‑1 (MQ=255)
                                                                                                ggCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                <  2:779243/151‑1 (MQ=255)
                                                                                                  ccGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCa                                              <  1:536952/151‑1 (MQ=255)
                                                                                                    gTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc                                            <  2:98607/151‑1 (MQ=255)
                                                                                                     ttGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                           >  2:837921/1‑151 (MQ=255)
                                                                                                       gCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                         <  1:607817/151‑1 (MQ=255)
                                                                                                        cTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATAGTTTCATTGCTGAGGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTACGACCGCTCGGTTCGTGGCCGATTACTCTATCCAGCTg                                          >  1:143569/1‑149 (MQ=17)
                                                                                                          ggCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCTGAGTACTCTATCCAGCTGAGCt                                      <  1:226346/151‑1 (MQ=255)
                                                                                                                  gTTATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGc                              <  2:570396/151‑1 (MQ=255)
                                                                                                                    tATCCTTCCTGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGc                              >  1:58082/1‑149 (MQ=255)
                                                                                                                     aTCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                           <  1:152900/151‑1 (MQ=255)
                                                                                                                        cttcctGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTAGGGGGAATATGGATCATCCGGGCGGCTTGGAACCTCCGAGCGCTCGGTTCGGAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCggg                        >  1:331324/1‑151 (MQ=14)
                                                                                                                          tcctGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCggg                        <  1:156521/149‑1 (MQ=255)
                                                                                                                          tcctGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGa                       <  1:75238/150‑1 (MQ=255)
                                                                                                                             tGGTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                          >  1:474590/1‑144 (MQ=255)
                                                                                                                               gTGCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACtt                 <  1:773637/151‑1 (MQ=255)
                                                                                                                                 gCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTAt               <  2:585723/151‑1 (MQ=255)
                                                                                                                                 gCCTGCGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTAt               <  1:57936/151‑1 (MQ=255)
                                                                                                                                      cGGTTAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTAc          <  2:64756/151‑1 (MQ=255)
                                                                                                                                          tAATACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTActgc       <  2:728495/150‑1 (MQ=255)
                                                                                                                                             tACCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTActgccggtt  >  1:177996/1‑146 (MQ=11)
                                                                                                                                              aCCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGata  <  2:635615/151‑1 (MQ=255)
                                                                                                                                              aCCGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCGTCGGGAAAACTTATTCTACTGCTGat   <  2:554216/150‑1 (MQ=255)
                                                                                                                                               ccGCCATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGGagatgtagttgtgt          >  1:740301/1‑129 (MQ=11)
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GATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATA  >  NC_000913/2817596‑2817888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: