Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,577	A→G	V812A (GTT→GCT)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,577	0	A	G	100.0%	167.5 / NA	47	V812A (GTT→GCT)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/27);  total (20/27)

AGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAACCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCT  >  NC_000913/2819429‑2819721                                                                                                                                                     |                                                                                                                                                 aGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcc                                                                                                                                                >  2:451632/1‑151 (MQ=255) aGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcc                                                                                                                                                >  1:493105/1‑151 (MQ=255)        gCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACTTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgccttacctt                                                                                                                                         <  2:137654/151‑1 (MQ=255)          cGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCg                                                                                                                                       >  2:44993/1‑151 (MQ=255)            cagcaTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGAc                                                                                                                                     >  2:371580/1‑151 (MQ=255)              gcaTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTa                                                                                                                                   <  1:194489/151‑1 (MQ=255)               caTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTa                                                                                                                                   <  2:533040/150‑1 (MQ=255)               caTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTAc                                                                                                                                  <  1:797934/151‑1 (MQ=255)                 tCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCg                                                                                                                                >  1:831788/1‑151 (MQ=255)                        caacgacTTGCGCCATGTCAGGACGTCCACCACCCTTGCCCCCCACCTGCTGAGCGCCCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGTAGCCTTACCTTCGACTACCGTTGcc                                                                                                                           >  1:223331/7‑149 (MQ=255)                            ggCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACg                                                                                                                     <  2:492349/151‑1 (MQ=255)                              cTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTGAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGAt                                                                                                                   >  1:25338/1‑151 (MQ=255)                                 gcgcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATaa                                                                                                                 <  1:284181/150‑1 (MQ=255)                                     cATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTc                                                                                                            >  2:59934/1‑151 (MQ=255)                                      aTGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCg                                                                                                           >  1:189035/1‑151 (MQ=255)                                      aTGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACAATAATTGTCg                                                                                                           >  1:595177/1‑151 (MQ=255)                                                gTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTg                                                                                                 >  1:529669/1‑151 (MQ=255)                                                     ccaccCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAtttt                                                                                            >  1:420996/1‑151 (MQ=255)                                                     ccaccCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAttt                                                                                             <  2:725503/150‑1 (MQ=255)                                                        cccTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTa                                                                                         <  2:673932/151‑1 (MQ=255)                                                            tGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTaaa                                                                                       <  2:127649/149‑1 (MQ=255)                                                             gccgccCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCg                                                                                    <  2:575055/151‑1 (MQ=255)                                                                   cACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAcc                                                                              <  1:44971/151‑1 (MQ=255)                                                                        gctgAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATg                                                                           <  1:612463/149‑1 (MQ=255)                                                                         ctgAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATgg                                                                          >  1:675496/1‑149 (MQ=255)                                                                            aGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGc                                                                     <  1:462829/151‑1 (MQ=255)                                                                              cGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCaa                                                                   <  1:303155/151‑1 (MQ=255)                                                                                   aTACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTaaa                                                                                       <  1:228205/126‑1 (MQ=255)                                                                                   aTACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTaaa                                                                                       >  2:228229/1‑126 (MQ=255)                                                                                     aCCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACAtttt                                                             <  1:135153/150‑1 (MQ=255)                                                                                     aCCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTc                                                            <  2:723941/151‑1 (MQ=255)                                                                                            aGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCaa                                                     <  1:692222/151‑1 (MQ=255)                                                                                            aGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCa                                                      <  2:771944/150‑1 (MQ=255)                                                                                             gTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAac                                                    <  2:500358/151‑1 (MQ=255)                                                                                             gTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAac                                                    >  2:591924/1‑151 (MQ=255)                                                                                                ccccTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAacac                                                  <  1:591886/150‑1 (MQ=255)                                                                                                     gCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTa                                             >  2:420684/1‑150 (MQ=255)                                                                                                         tCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCt                                         <  2:642863/150‑1 (MQ=255)                                                                                                         tCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTc                                        >  2:145340/1‑151 (MQ=255)                                                                                                            cacGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCt                                     <  2:493141/151‑1 (MQ=255)                                                                                                               gATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAAc                                  >  2:313866/1‑151 (MQ=255)                                                                                                                    gtgACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCaa                               <  1:577938/149‑1 (MQ=255)                                                                                                                           ccTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTaa                       <  2:596726/150‑1 (MQ=255)                                                                                                                                    cGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAAc             <  2:189058/151‑1 (MQ=255)                                                                                                                                    cGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAAc             >  1:716846/1‑151 (MQ=255)                                                                                                                                      ccTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACAt           >  1:74844/1‑151 (MQ=255)                                                                                                                                       cTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATc          >  1:730181/1‑151 (MQ=255)                                                                                                                                         gCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTATCCAACAGCTTAACACCAGTAACATCaa        <  1:243032/151‑1 (MQ=255)                                                                                                                                         gCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCaa        <  2:185575/151‑1 (MQ=255)                                                                                                                                               agagAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCt  >  1:143190/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 AGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAACCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCT  >  NC_000913/2819429‑2819721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑