Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	359,143	T→C	H115H (CAT→CAC)	cynT →	carbonic anhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	359,143	0	T	C	100.0%	157.4 / NA	43	H115H (CAT→CAC)	cynT	carbonic anhydrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/17);  total (26/17)

GTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAAC  >  NC_000913/358997‑359283                                                                                                                                                   |                                                                                                                                             gTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACAtgc                                                                                                                                          >  2:140931/1‑151 (MQ=255)   cccTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACAtgcct                                                                                                                                        >  1:342414/1‑151 (MQ=255)   cccTGCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTGCGGTGGGGTAGGCCGTCCCGGCGCTTCGGGTATCTCACATGGTGATTTGTGGTCGTCCCAGCTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACAtgcct                                                                                                                                        <  1:170810/151‑1 (MQ=255)     cTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGCGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACAagcctgc                                                                                                                                      >  1:469259/1‑151 (MQ=255)            cGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTcc                                                                                                                               <  2:823453/151‑1 (MQ=255)                  ggAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCCTCGGGGATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATACCTGCCGACTCCCAc                                                                                                                            >  1:274001/1‑148 (MQ=255)                   gAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGc                                                                                                                        >  2:689126/1‑151 (MQ=255)                    aaCCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCt                                                                                                                       <  2:196568/151‑1 (MQ=255)                            ggCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATg                                                                                                               <  1:143745/151‑1 (MQ=255)                              cGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGcc                                                                                                             >  1:635080/1‑151 (MQ=255)                                       ttCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGcc                                                                                                    >  1:379322/1‑151 (MQ=255)                                         cgttGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACTTTGTGATTTGTGGTCATGCCAACTGGGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAg                                                                                                      <  1:466033/144‑1 (MQ=255)                                         cGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGccc                                                                                                   <  2:500313/150‑1 (MQ=255)                                         cGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGcc                                                                                                    <  2:4979/149‑1 (MQ=255)                                          ggTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCcgc                                                                                                 >  2:643059/1‑151 (MQ=255)                                                 gaTGCGGTCGATGCGCTGCGGGGATCTGACATTTTGATTTGTGGTCATTCCAACTGTGGCGGGATGACCGCCATTGTCAACTGTCAGTGCATGGTCCACATGCCTGCCGTCTCCCTCTGGCTGCGTTATGCCGATTCAGCCCGCGTCGtt                                                                                           <  1:62019/149‑1 (MQ=255)                                                      cGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGAACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAAt                                                                                        >  2:734197/1‑148 (MQ=255)                                                         cGCTGCGCTCCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAgg                                                                                    <  2:44094/149‑1 (MQ=255)                                                           cTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGgcgcg                                                                                >  1:713362/1‑151 (MQ=255)                                                             gcgcTTCTGGTATCTGACATTGTGATTTGTGGCCATTCCAACTGTGGCGCGATAACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGCCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTACTCAGGCGCGcc                                                                              >  2:846463/1‑151 (MQ=255)                                                                   cGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCAt                                                                         <  2:660440/150‑1 (MQ=255)                                                                    gggTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTc                                                                       >  2:802043/1‑151 (MQ=255)                                                                        aTCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGa                                                                    <  2:656654/150‑1 (MQ=255)                                                                        aTCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGAt                                                                   >  2:670680/1‑151 (MQ=255)                                                                            gACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGTCCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTa                                                                <  1:603746/150‑1 (MQ=255)                                                                            gACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTAc                                                               >  2:3553/1‑151 (MQ=255)                                                                               aTTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGt                                                            >  1:700435/1‑151 (MQ=255)                                                                                    gATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCaaa                                                        <  2:56906/150‑1 (MQ=255)                                                                                           ggTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTgcgg                                                <  2:717165/151‑1 (MQ=255)                                                                                             tCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTgcggcg                                              >  1:86206/1‑151 (MQ=255)                                                                                                  ccAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGt                                         <  1:845795/151‑1 (MQ=255)                                                                                                    aaCTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTAc                                       >  1:10473/1‑151 (MQ=255)                                                                                                                  gACCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCAtt                         >  2:229873/1‑151 (MQ=255)                                                                                                                   aCCGCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTg                        >  2:131409/1‑151 (MQ=255)                                                                                                                      gCCATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTc                     >  2:600561/1‑151 (MQ=255)                                                                                                                       ccATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCa                    <  2:112024/151‑1 (MQ=255)                                                                                                                        cATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTc                     >  2:626119/1‑149 (MQ=255)                                                                                                                        cATTGCCAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTc                     >  2:318100/1‑149 (MQ=255)                                                                                                                              cAGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCt             >  2:574315/1‑151 (MQ=255)                                                                                                                               aGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCt             >  1:359337/1‑150 (MQ=255)                                                                                                                               aGCTGTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCt             >  2:122050/1‑150 (MQ=255)                                                                                                                                   gTCAGTGCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAAttt        >  1:807284/1‑151 (MQ=255)                                                                                                                                         gCATGGACCACATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAAc  <  2:427598/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                             GTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCGGTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTGCAAAC  >  NC_000913/358997‑359283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑