Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,955,448	T→C	T186A (ACC→GCC)	recB ←	exonuclease V (RecBCD complex), beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,955,448	0	T	C	100.0%	182.0 / NA	50	T186A (ACC→GCC)	recB	exonuclease V (RecBCD complex), beta subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/29);  total (21/29)

TGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGA  >  NC_000913/2955300‑2955594                                                                                                                                                     |                                                                                                                                                   tgctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGctt                                                                                                                                                  >  2:526431/1‑151 (MQ=255)  gctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTc                                                                                                                                                 >  1:407729/1‑151 (MQ=255)   ctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTc                                                                                                                                                 >  1:355003/1‑150 (MQ=255)       tttACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAg                                                                                                                                             <  1:628176/150‑1 (MQ=255)             gTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCt                                                                                                                                      <  2:379460/151‑1 (MQ=255)             gTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCt                                                                                                                                      <  1:244344/151‑1 (MQ=255)               aTCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTgg                                                                                                                                    <  2:712539/151‑1 (MQ=255)                  aaTACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCt                                                                                                                                 >  1:152973/1‑151 (MQ=255)                     aCGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTAtt                                                                                                                              >  2:812146/1‑151 (MQ=255)                     aCGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTAt                                                                                                                               <  2:198870/150‑1 (MQ=255)                                tttGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGc                                                                                                                     >  2:203392/1‑149 (MQ=255)                                       gTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAg                                                                                                            <  1:526153/151‑1 (MQ=255)                                         gACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc                                                                                                           >  1:857081/1‑150 (MQ=255)                                           cGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGt                                                                                                        <  1:203369/151‑1 (MQ=255)                                                     gCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCa                                                                                              <  1:305338/151‑1 (MQ=255)                                                        tttCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGa                                                                                            >  1:26351/1‑150 (MQ=255)                                                         ttCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTAtt                                                                                                                              <  2:737914/115‑1 (MQ=255)                                                         ttCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTAtt                                                                                                                              >  1:737871/1‑115 (MQ=255)                                                                  gggCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAg                                                                                 <  1:283210/151‑1 (MQ=255)                                                                  gggCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAg                                                                                 <  2:663342/151‑1 (MQ=255)                                                                  gggCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAg                                                                                 <  2:369941/151‑1 (MQ=255)                                                                               ttGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGgtagcg                                                                      <  2:689980/149‑1 (MQ=255)                                                                                tGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGgtagcgt                                                                     >  1:228838/1‑149 (MQ=255)                                                                                  aTAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGgtagcgtagc                                                                  >  1:129556/1‑150 (MQ=255)                                                                                          cgcTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTc                                                          >  1:417047/1‑150 (MQ=255)                                                                                          cgcTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCa                                                         <  2:805206/151‑1 (MQ=255)                                                                                               cGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCtt                                                     <  2:334646/150‑1 (MQ=255)                                                                                                    tGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATc                                                <  2:706527/150‑1 (MQ=255)                                                                                                     gCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAg                                              <  2:633823/151‑1 (MQ=255)                                                                                                       aGATAACGATTAATATCGCGCAGCAACGCCTGCGGCGCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAgct                                            >  2:575618/1‑151 (MQ=255)                                                                                                           aaCGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAgctgct                                         <  2:792558/150‑1 (MQ=255)                                                                                                           aaCGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGAAGCGTAGCAGAGACTCATCTTCAATCAgctgctg                                        >  1:382557/1‑151 (MQ=255)                                                                                                            aCGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAgctgctgc                                       <  1:779381/151‑1 (MQ=255)                                                                                                              gATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTc                                     <  1:442368/151‑1 (MQ=255)                                                                                                               aTTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCa                                    <  2:661203/151‑1 (MQ=255)                                                                                                               aTTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCa                                    <  1:724042/151‑1 (MQ=255)                                                                                                                 tAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCaa                                   >  2:795906/1‑150 (MQ=255)                                                                                                                  aaTATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCaaa                                  <  1:548196/150‑1 (MQ=255)                                                                                                                  aaTATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAAc                                 <  2:407761/151‑1 (MQ=255)                                                                                                                   atatCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCaaa                                  <  2:459232/149‑1 (MQ=255)                                                                                                                     atCGCGCAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGc                              >  2:617545/1‑151 (MQ=255)                                                                                                                        gcgcAGCAACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATg                           >  2:106923/1‑151 (MQ=255)                                                                                                                          gcagcaACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGcc                         >  1:668283/1‑151 (MQ=255)                                                                                                                           cagcaACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCg                        >  2:564226/1‑151 (MQ=255)                                                                                                                              caACGCCTGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGAt                     <  2:448891/151‑1 (MQ=255)                                                                                                                                     tGCGGCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAgg              >  1:706178/1‑151 (MQ=255)                                                                                                                                         gCCCTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCAtt          <  1:637244/151‑1 (MQ=255)                                                                                                                                           ccTTTCCAGGCTTCAAAGTCGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCa        <  1:235206/151‑1 (MQ=255)                                                                                                                                            cTTTCCAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTc         >  2:705444/1‑149 (MQ=255)                                                                                                                                              ttCCAGGCTTCAAAGACGACCTTGGCTATTTCACGCGGCAGCGGGGATCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCTGGATTCAAAGGCATTCAGGt     >  1:460999/1‑151 (MQ=255)                                                                                                                                                 cAGGCTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGa  <  1:811505/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCTCAAACAGCATGCCGGATTCAAAGGCATTCAGGTTGA  >  NC_000913/2955300‑2955594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑