Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,989,983	A→G	A126A (GCT→GCC)	yqeK ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,989,983	0	A	G	100.0%	189.2 / NA	52	A126A (GCT→GCC)	yqeK	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/30);  total (22/30)

AAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATC  >  NC_000913/2989835‑2990129                                                                                                                                                     |                                                                                                                                                   aaaaTTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGc                                                                                                                                                  >  1:734625/1‑151 (MQ=255)   aaTTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTc                                                                                                                                                >  2:61514/1‑151 (MQ=255)        aaTATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGatta                                                                                                                                           <  1:114303/151‑1 (MQ=255)        aaTATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGatta                                                                                                                                           <  2:595290/151‑1 (MQ=255)         atatTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGattat                                                                                                                                          <  2:73691/151‑1 (MQ=255)           atTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATCTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACTTTCATCTTGGCTCGattatt                                                                                                                                         <  1:520199/150‑1 (MQ=255)                cGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTc                                                                                                                                     <  1:843831/149‑1 (MQ=255)                cGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCt                                                                                                                                   >  2:437406/1‑151 (MQ=255)                          ggCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGt                                                                                                                          <  2:828694/150‑1 (MQ=255)                          ggCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGt                                                                                                                          <  2:709356/150‑1 (MQ=255)                          ggCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTc                                                                                                                         <  1:716365/151‑1 (MQ=255)                             aGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGAt                                                                                                                      >  2:538159/1‑151 (MQ=255)                               tCTAAATCATCAAAGATTCGATCAACATCTTTCATTTTAGACATCTCCTGGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGAGTATTATTCCTTCGGACAGTCGTTg                                                                                                                     <  1:17278/150‑1 (MQ=255)                                    aGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAAc                                                                                                                >  2:548978/1‑150 (MQ=255)                                         cAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACa                                                                                                          <  1:319327/151‑1 (MQ=255)                                         cAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACa                                                                                                          <  2:613270/151‑1 (MQ=255)                                           aaGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGa                                                                                                        <  1:496542/151‑1 (MQ=255)                                             gATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGaaa                                                                                                      <  2:454948/151‑1 (MQ=255)                                                 tGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAagag                                                                                                   <  1:393507/150‑1 (MQ=255)                                                 tGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAagag                                                                                                   <  2:503930/150‑1 (MQ=255)                                                  gATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAgg                                                                                                  >  2:188204/1‑150 (MQ=255)                                                  gATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAgg                                                                                                  >  2:392784/1‑150 (MQ=255)                                                        cATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGattatt                                                                                            >  1:197881/1‑150 (MQ=255)                                                         aTCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGAttatta                                                                                           >  2:588397/1‑150 (MQ=255)                                                          tCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGAttatta                                                                                           >  1:410637/1‑149 (MQ=255)                                                           cTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGAttatta                                                                                           >  2:226294/1‑148 (MQ=255)                                                               cATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCa                                                                                    <  1:519454/151‑1 (MQ=255)                                                                    tAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGttt                                                                                >  2:718462/1‑150 (MQ=255)                                                                          tctcCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAg                                                                          >  1:248243/1‑150 (MQ=255)                                                                                tGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAAt                                                                     <  1:809268/149‑1 (MQ=255)                                                                                  cAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCaa                                                                  <  2:626278/150‑1 (MQ=255)                                                                                    aTGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATag                                                               <  1:72553/151‑1 (MQ=255)                                                                                      gTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATagag                                                             <  1:165066/151‑1 (MQ=255)                                                                                      gTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATaga                                                              <  2:531254/150‑1 (MQ=255)                                                                                       tAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAgg                                                            >  2:240446/1‑151 (MQ=255)                                                                                         aaaTATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATAc                                                                                                           <  2:229851/102‑1 (MQ=255)                                                                                         aaaTATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATAc                                                                                                           >  1:229827/1‑102 (MQ=255)                                                                                          aaTATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGaa                                                          >  1:125566/1‑150 (MQ=255)                                                                                           atatCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGGAAGAGGATTCTTAAGAACCAGTTTACTCAGTGAATCAATAGCGGaa                                                          >  2:527654/1‑149 (MQ=255)                                                                                                  gTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTaa                                                  <  2:191373/150‑1 (MQ=255)                                                                                                         caacaTCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCtt                                          <  1:629950/151‑1 (MQ=255)                                                                                                          aacaTCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCttt                                         <  1:706177/151‑1 (MQ=255)                                                                                                               cTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTaaa                                      >  1:537052/1‑149 (MQ=255)                                                                                                                 ggTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTaaa                                      <  1:771232/147‑1 (MQ=255)                                                                                                                 ggTGCAAATCCATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGaa                                  <  1:540542/151‑1 (MQ=255)                                                                                                                           cATGAGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTtcat                        <  1:429387/151‑1 (MQ=255)                                                                                                                               aGTCGAACTCGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTcatcat                     >  1:284843/1‑150 (MQ=255)                                                                                                                                        cGACATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTtaaac            <  1:511551/150‑1 (MQ=255)                                                                                                                                           cATTCATCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTtaaactaa         >  2:816038/1‑150 (MQ=255)                                                                                                                                            aTTCATCTTGGCTCGATTATTATTCCTTCGTATAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTtaaactaaac       <  2:192899/151‑1 (MQ=255)                                                                                                                                                 tCTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACtcatc  >  1:615322/1‑151 (MQ=255)                                                                                                                                                  cTTGGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACtcatc  >  1:566668/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATC  >  NC_000913/2989835‑2990129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑