| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 373,704 | G→A | V262M (GTG→ATG) | mhpF → | acetaldehyde‑CoA dehydrogenase II, NAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 373,704 | 0 | G | A | 98.3% | 205.8 / NA | 58 | V262M (GTG→ATG) | mhpF | acetaldehyde‑CoA dehydrogenase II, NAD‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (28/29); minor base C (1/0); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGC > NC_000913/373559‑373850 | gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATggg < 1:825449/150‑1 (MQ=255) acgaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAAtt < 1:230241/151‑1 (MQ=255) acgaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAAAAGCGCGTGCAGTTTGAAGGTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAAtt > 1:393376/1‑151 (MQ=255) gaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATtct < 1:684924/151‑1 (MQ=255) gaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATtct < 1:321972/151‑1 (MQ=255) aaGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGGAGGCTTACGTTCCCGGGTTTCGCCTGGAAAAGGGCGTGCAGGTTGAAGGTGTCCCCCAGGGTAAAACGGGAAATTTTCCGGGGATGGGGCCAtttt > 1:342915/1‑148 (MQ=255) aaGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATtctc < 2:514377/151‑1 (MQ=255) gCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTcc < 1:817884/150‑1 (MQ=255) cacaAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGAc < 1:782114/150‑1 (MQ=255) cacaAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAACGCGTGCAGTTTGAAGTTATCCCGCAGGAAAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGAc > 2:673878/1‑150 (MQ=255) cacaAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAAAAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACt > 2:859088/1‑151 (MQ=255) caAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGGCAATTTACCGGGCATGGGGCAATTCTCCGGACt > 1:442278/1‑149 (MQ=255) aGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCCGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAAACGGGCAATTTACCGGGCATGGGGCAATTCTCCGGAc > 1:784268/1‑146 (MQ=255) gatATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAg < 1:85899/151‑1 (MQ=255) atatCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGc < 2:489092/151‑1 (MQ=255) atCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCg < 1:378589/150‑1 (MQ=255) aGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTctgg > 1:367570/1‑151 (MQ=255) caatcaatGAAATGGCTGAGGCGGTTCAGGGTTACGTACCGGGTTATCGCCTGAAACAACGCGTGCAGGTTGGAGATATCCCGCAGGATAAACCGGTCAATTTACCGGGCCTAGGGCAATTCTCCGGACTGAAAACAGAGGTctttct > 1:319669/1‑144 (MQ=255) caatcaatGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTctggctg < 1:499130/149‑1 (MQ=255) caatGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGt > 2:404889/1‑150 (MQ=255) caatGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTc < 1:754197/151‑1 (MQ=255) aaaTGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAgg < 1:531963/151‑1 (MQ=255) aaTGGCTGAGGCGGTGCAGGCTTAAGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAAACGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAAAAGCGGTCTGGCTGGAAGTCGAAgg > 2:368915/1‑150 (MQ=255) cTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcgcag > 2:466444/1‑150 (MQ=255) gAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcgcagcgc > 2:282567/1‑151 (MQ=255) gAGGCGGTGCAGGCCTACGTACCGGGGTATCGCCCGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGGTAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAAAAACGGTCTGGCTGGAAGGCGAAGgcgccgcgc > 1:369822/1‑151 (MQ=255) ggCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCAt < 1:723754/151‑1 (MQ=255) cAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCATCGCATTATctgc < 1:53111/151‑1 (MQ=255) cAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATctgc > 1:647284/1‑151 (MQ=255) gCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATctgcctg > 1:74227/1‑151 (MQ=255) tACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTgcctgcct > 2:603048/1‑151 (MQ=255) aCGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTa < 2:755032/151‑1 (MQ=255) aCGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTa < 2:367593/151‑1 (MQ=255) gTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTAt < 1:674222/150‑1 (MQ=255) gTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATg < 2:838951/151‑1 (MQ=255) aCCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGc < 2:475840/150‑1 (MQ=255) aCCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCg < 2:773007/151‑1 (MQ=255) aCCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCg > 2:37384/1‑151 (MQ=255) cGGGTTATAGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGAGAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAACCAGCGGTCTGGCTAGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCggg > 1:212573/1‑151 (MQ=255) cGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCAGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTc < 1:450996/150‑1 (MQ=255) gTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTcc > 1:692521/1‑150 (MQ=255) tGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTcc > 2:33329/1‑149 (MQ=255) tGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCt < 2:393408/150‑1 (MQ=255) gAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTgg > 1:157553/1‑151 (MQ=255) aaGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGc < 2:594977/151‑1 (MQ=255) aGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCg < 2:325181/151‑1 (MQ=255) ttATCCCGCAGGATAAACCGGGCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGAc > 2:424767/1‑151 (MQ=255) aTCCCGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGAc > 2:620065/1‑149 (MQ=255) cGCAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGa < 2:189589/151‑1 (MQ=255) cAGGATAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGaa < 1:18913/150‑1 (MQ=255) aTAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGGCGAAGGAGCAGCGCATTATCTGCCTGCCTATGCGGGCAAACTCGACATTATGACTTCCAGTGCGCGGGCGACAGCGGAAAAAAt > 1:701604/1‑151 (MQ=255) tAAACCGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATg > 1:319305/1‑151 (MQ=255) aaaCCGGTCAATTTACCGGGCATAGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATgg < 1:703055/151‑1 (MQ=255) cGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGccc > 1:320831/1‑150 (MQ=255) cGGTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGccc > 1:36239/1‑150 (MQ=255) gTCAATTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGt < 1:314800/151‑1 (MQ=255) aTTTACCGGGCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACt > 1:528821/1‑151 (MQ=255) gggCATGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcgcgc > 1:541907/1‑151 (MQ=255) | GAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGC > NC_000913/373559‑373850 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |