Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,012,391	T→C	*311Q (TAA→CAA)	yqeA →	putative amino acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,012,391	0	T	C	100.0%	241.3 / NA	63	*311Q (TAA→CAA)	yqeA	putative amino acid kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/33);  total (30/33)

TTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCC  >  NC_000913/3012250‑3012532                                                                                                                                              |                                                                                                                                              tttGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTaa                                                                                                                                        >  2:406795/1‑149 (MQ=255)     cGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCg                                                                                                                                  <  2:725985/151‑1 (MQ=255)      gAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCgg                                                                                                                                 <  1:360055/151‑1 (MQ=255)        aaGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCgggt                                                                                                                               >  1:446903/1‑151 (MQ=255)        aaGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCgggt                                                                                                                               <  2:477699/151‑1 (MQ=255)                aTGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGATCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTaa                                                                                                                         <  1:684750/149‑1 (MQ=255)                aTGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAAt                                                                                                                        >  2:378541/1‑150 (MQ=255)                aTGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATa                                                                                                                       <  1:522552/151‑1 (MQ=255)                   ctgcCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATAcc                                                                                                                     >  1:96251/1‑150 (MQ=255)                      ccTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATcc                                                                                                                 <  2:721343/151‑1 (MQ=255)                         aaaGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCg                                                                                                                <  2:669641/149‑1 (MQ=255)                                aaGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCaa                                                                                                           >  1:731235/1‑147 (MQ=255)                                 aGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCaaa                                                                                                          <  1:392973/147‑1 (MQ=255)                                 aGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCaaa                                                                                                          >  2:393005/1‑147 (MQ=255)                                 aGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGAtt                                                                                                      >  2:700651/1‑151 (MQ=255)                                 aGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGAtt                                                                                                      <  1:388037/151‑1 (MQ=255)                                      ccgccTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCaa                                                                                                 <  1:444369/151‑1 (MQ=255)                                      ccgccTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCaa                                                                                                 <  2:126777/151‑1 (MQ=255)                                      ccgccTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCaa                                                                                                 <  2:778953/151‑1 (MQ=255)                                      ccgccTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCa                                                                                                  >  2:179954/1‑150 (MQ=255)                                       cgccTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCaag                                                                                                <  1:3658/151‑1 (MQ=255)                                          cTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCaagta                                                                                              >  1:366188/1‑150 (MQ=255)                                             gTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCaagtaagt                                                                                           <  1:431929/150‑1 (MQ=255)                                             gTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTg                                                                                          >  1:816218/1‑151 (MQ=255)                                                  ctcgctcTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCtt                                                                                     >  1:547363/1‑151 (MQ=255)                                                    cgctcTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTttg                                                                                   >  1:519553/1‑151 (MQ=255)                                                            cgcAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGcc                                                                           <  2:748438/151‑1 (MQ=255)                                                              cAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCgg                                                                         <  2:81477/151‑1 (MQ=255)                                                                ggTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGAt                                                                       <  1:594048/151‑1 (MQ=255)                                                                   cGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATgcg                                                                    >  2:821527/1‑151 (MQ=255)                                                                   cGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATgc                                                                     <  1:635617/150‑1 (MQ=255)                                                                    gTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATgcgg                                                                   >  1:324710/1‑151 (MQ=255)                                                                     tGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATgcggc                                                                  >  2:476243/1‑151 (MQ=255)                                                                     tGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATgc                                                                     >  1:404251/1‑148 (MQ=255)                                                                     tGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATgc                                                                     <  2:404283/148‑1 (MQ=255)                                                                        aGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGcgcg                                                               <  2:421732/151‑1 (MQ=255)                                                                          cACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGcgcg                                                               >  2:736552/1‑149 (MQ=255)                                                                           aCTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAgc                                                            <  2:485042/151‑1 (MQ=255)                                                                              gATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAgcg                                                           <  1:415534/149‑1 (MQ=255)                                                                               aTTACCGAATTGAGCACAGCGAAAGAAGGGATTGAAGGCAACACAGGAACGGTGATTTGTCAGCAATTTAAAGGGCGGGTGGGCAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCAGATGCGTCGCGAGCGCCt                                                        >  1:21024/1‑151 (MQ=255)                                                                                          gAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCt                                              >  2:408824/1‑150 (MQ=255)                                                                                              aaaGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACaaa                                         <  2:405250/151‑1 (MQ=255)                                                                                               aaGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACaaaa                                        <  1:736509/151‑1 (MQ=255)                                                                                                aGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAAc                                       >  1:405229/1‑151 (MQ=255)                                                                                                    aaaGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCg                                   >  2:387988/1‑151 (MQ=255)                                                                                                     aagaagGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGc                                  >  1:730668/1‑151 (MQ=255)                                                                                                      agaagGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGc                                  >  2:103781/1‑150 (MQ=255)                                                                                                       gaagGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCa                                 <  2:529722/150‑1 (MQ=255)                                                                                                          gggATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAAtt                             <  1:103758/151‑1 (MQ=255)                                                                                                          gggATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAAtt                             >  2:199154/1‑151 (MQ=255)                                                                                                            gATTGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCa                           <  1:807444/151‑1 (MQ=255)                                                                                                              ttGAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAAt                         >  2:165206/1‑151 (MQ=255)                                                                                                                gAAGGCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATa                        <  2:165809/150‑1 (MQ=255)                                                                                                                   ggCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGAtt                    <  2:461999/151‑1 (MQ=255)                                                                                                                    gCAAAACAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTg                   <  1:524448/151‑1 (MQ=255)                                                                                                                     cAAAACAGGAACGGTGATTTGCCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGc                  >  2:621708/1‑151 (MQ=255)                                                                                                                      aaaaCAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCa                 <  1:181267/151‑1 (MQ=255)                                                                                                                          cAGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGc             <  1:540674/151‑1 (MQ=255)                                                                                                                           aGGAACGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGACGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGc             >  1:774068/1‑150 (MQ=255)                                                                                                                               aCGGTGATTTGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTg        <  2:109094/151‑1 (MQ=255)                                                                                                                                   caaTTTGTCAGCAATTTAAAGGCCGGGAGGGTAATACCATCCGACCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAATCATCGCAAATTCAATAGATTGCACAGCACtagtag     >  1:795303/3‑150 (MQ=255)                                                                                                                                      tttGTCAGCAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATTCCGGATGCGTCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGcc  >  2:642544/1‑150 (MQ=255)                                                                                                                                       ttGTCAGCAATTTAAAGGCAGGGTGGTGAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTCTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGGAGGc   >  1:660502/1‑148 (MQ=39)                                                                                                                                              |                                                                                                                                              TTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGTCAGTAATTTAAAGGCCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCC  >  NC_000913/3012250‑3012532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑