Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,166	A→G	Y36C (TAC→TGC)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,166	0	A	G	100.0%	204.4 / NA	56	Y36C (TAC→TGC)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/21);  total (35/21)

CGGTCACTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCG  >  NC_000913/3016020‑3016312                                                                                                                                                   |                                                                                                                                                   cGGTCACTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTc                                                                                                                                                 >  1:584152/1‑150 (MQ=255)      aCTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGt                                                                                                                                           >  1:142024/1‑151 (MQ=255)      aCTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGt                                                                                                                                           >  1:351506/1‑151 (MQ=255)         cAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTaa                                                                                                                                         <  1:507988/150‑1 (MQ=255)             gATATTTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAg                                                                                                                                      >  1:225931/1‑149 (MQ=255)                   tGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACt                                                                                                                              >  1:838087/1‑151 (MQ=255)                    gTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACt                                                                                                                              <  2:74107/150‑1 (MQ=255)                      ataAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGtt                                                                                                                           <  2:465585/151‑1 (MQ=255)                               ggAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTc                                                                                                                  >  1:230080/1‑151 (MQ=255)                                     ggTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGa                                                                                                             >  2:268421/1‑150 (MQ=255)                                           gggggATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCtgt                                                                                                      >  1:590419/1‑151 (MQ=255)                                                 tattatGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACt                                                                                                >  2:375595/1‑151 (MQ=255)                                                   ttatGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTcc                                                                                              >  1:216868/1‑151 (MQ=255)                                                       gCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTc                                                                                          <  1:61278/151‑1 (MQ=255)                                                        cGTCCCATTCCTTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACTCTCAATCTTTGGTATTCCCGAGCAACAGTGTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGt                                                                                           <  2:221947/149‑1 (MQ=255)                                                              aTTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTg                                                                                   >  2:795082/1‑151 (MQ=255)                                                              aTTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTg                                                                                   <  2:602370/151‑1 (MQ=255)                                                                 ccGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGcc                                                                                 <  1:42133/150‑1 (MQ=255)                                                                 ccGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGcc                                                                                 <  2:437187/150‑1 (MQ=255)                                                                 ccGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCg                                                                                >  2:277633/1‑151 (MQ=255)                                                                   gTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCt                                                                              >  2:283760/1‑151 (MQ=255)                                                                          gAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGc                                                                       >  1:704383/1‑151 (MQ=255)                                                                               tttGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCacac                                                                   <  2:621649/150‑1 (MQ=255)                                                                               tttGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCacac                                                                   <  2:369477/150‑1 (MQ=255)                                                                                   aCGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGc                                                              >  2:551602/1‑151 (MQ=255)                                                                                     gcgcATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTc                                                            <  2:322429/151‑1 (MQ=255)                                                                                       gcATATTTGATGAATACAAACAACAACGCGCAATCTTTGGCATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCACGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTc                                                            >  1:26555/1‑149 (MQ=255)                                                                                        cATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTcgcg                                                         <  1:53130/151‑1 (MQ=255)                                                                                          tatTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCgcgc                                                        >  1:340650/1‑150 (MQ=255)                                                                                             ttGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCaa                                                      >  1:408545/1‑149 (MQ=255)                                                                                               gatgaATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAata                                                  <  1:192864/151‑1 (MQ=255)                                                                                                atgaATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAatat                                                 >  2:858666/1‑151 (MQ=255)                                                                                                atgaATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAatat                                                 >  2:139611/1‑151 (MQ=255)                                                                                                atgaATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAatat                                                 >  1:677687/1‑151 (MQ=255)                                                                                                      aCCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCAc                                           >  1:313919/1‑151 (MQ=255)                                                                                                       ccAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCAc                                           <  2:12412/150‑1 (MQ=255)                                                                                                          acaacaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTcc                                       >  1:615618/1‑151 (MQ=255)                                                                                                           caacaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCt                                      >  2:5931/1‑151 (MQ=255)                                                                                                               aacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCt                                  <  2:691667/151‑1 (MQ=255)                                                                                                                    tCAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTg                             >  2:747014/1‑151 (MQ=255)                                                                                                                     cAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACt                              <  2:200694/149‑1 (MQ=255)                                                                                                                     cAATCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTgg                            >  2:133910/1‑151 (MQ=255)                                                                                                                      aaTCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGc                           >  1:582014/1‑151 (MQ=255)                                                                                                                      aaTCTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGc                           <  1:833695/151‑1 (MQ=255)                                                                                                                         cTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCgg                         >  2:220626/1‑150 (MQ=255)                                                                                                                         cTTTGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCg                          >  1:578892/1‑149 (MQ=255)                                                                                                                          tttGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGAc                       >  2:570886/1‑151 (MQ=255)                                                                                                                           ttGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGAc                       <  2:355609/150‑1 (MQ=255)                                                                                                                            tGGTATTCCCGAGCAACAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACg                      <  1:268396/150‑1 (MQ=255)                                                                                                                                         cAACAGTTTTGCACACCTGTAATAGGTAAAACTGTTGACGTCTCCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGACGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTCCCTGGCTGGCTGGCGGACGCTTCATCGAACTa         <  1:129224/150‑1 (MQ=255)                                                                                                                                          aaCAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTaa        >  1:731144/1‑150 (MQ=255)                                                                                                                                           aCAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCGGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTTATCGAACTaa        >  1:387/1‑149 (MQ=255)                                                                                                                                            cAGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCCGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTaaaa      >  1:564894/1‑150 (MQ=255)                                                                                                                                             aGTTTTGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAAc    >  1:709075/1‑151 (MQ=255)                                                                                                                                               ttttGCTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCg  >  1:392794/1‑151 (MQ=255)                                                                                                                                               ttttGCTCACCTGTAAAAGGTAAAACGTTTAGCGTCTTCCGTAAAACCTGTGCCACACCAGCCGGCCCTGCCGCTGGCCCGCGCACGCATCTCGCCCAAAATATTGTCACTACCTGGCTGACTGGCGGACGTTTCATCGAACTAAAAAcc   <  1:822887/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGGTCACTCAATGATATCTGTATAAGCTAAGGAGAGGGTTATGGGGGATATTATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCG  >  NC_000913/3016020‑3016312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑