Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,019,115	T→C	V1019A (GTC→GCC)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,019,115	0	T	C	100.0%	152.5 / NA	42	V1019A (GTC→GCC)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/18);  total (24/18)

ACAGCAGCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGTCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCGCTATCGG  >  NC_000913/3018968‑3019261                                                                                                                                                    |                                                                                                                                                   aCAGCAGCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGccc                                                                                                                                                  >  1:203811/1‑150 (MQ=255) aCAGCAGCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGccc                                                                                                                                                  >  1:437527/1‑150 (MQ=255)  cagcagCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCccac                                                                                                                                                >  1:60773/1‑151 (MQ=255)      agcaACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGc                                                                                                                                            <  2:745897/151‑1 (MQ=255)       gcaACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGc                                                                                                                                            >  2:595369/1‑150 (MQ=255)        caACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGcat                                                                                                                                          >  2:714093/1‑151 (MQ=255)            ccAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGcatcatc                                                                                                                                      <  2:698333/151‑1 (MQ=255)              aGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGcatcatcat                                                                                                                                    <  2:51459/151‑1 (MQ=255)               ggCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCAtt                                                                                                                                   >  1:24279/1‑151 (MQ=255)                gCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCAtt                                                                                                                                   >  1:172550/1‑150 (MQ=255)                   tCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATc                                                                                                                                <  2:589238/150‑1 (MQ=255)                    ccTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATctg                                                                                                                              <  1:307027/151‑1 (MQ=255)                               aTTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGt                                                                                                                   >  2:594938/1‑151 (MQ=255)                                  gCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTgg                                                                                                                 >  1:141405/1‑150 (MQ=255)                                    cGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAgg                                                                                                              <  1:843246/151‑1 (MQ=255)                                      ggTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGgtg                                                                                                            <  1:631144/151‑1 (MQ=255)                                      ggTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGgt                                                                                                             <  2:54671/150‑1 (MQ=255)                                            aGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATc                                                                                                       >  2:49803/1‑150 (MQ=255)                                             gTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCAt                                                                                                     <  1:557260/151‑1 (MQ=255)                                                 gTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTg                                                                                                 >  2:637325/1‑151 (MQ=255)                                                     gAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTc                                                                                             >  2:707318/1‑151 (MQ=255)                                                      gctcttccgatcTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCt                                                                                            >  2:104904/12‑151 (MQ=255)                                                         aaCCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTgaa                                                                                         <  2:490313/151‑1 (MQ=255)                                                          aCCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACCACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGCTGTAATCATGTTGCTTCTg                                                                                           >  1:3198/1‑148 (MQ=255)                                                              aaGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATg                                                                                    >  1:168089/1‑151 (MQ=255)                                                                   gggTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCa                                                                                 <  2:193138/149‑1 (MQ=255)                                                                    ggTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTg                                                                              >  1:705082/1‑151 (MQ=255)                                                                          aaaCATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTAc                                                                        >  2:43605/1‑151 (MQ=255)                                                                                 gACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTac                                                                  >  1:703991/1‑150 (MQ=255)                                                                                  aCAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTacac                                                                <  2:835409/151‑1 (MQ=255)                                                                                    aGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACAccc                                                              <  1:484403/151‑1 (MQ=255)                                                                                        aaGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGc                                                           >  1:581714/1‑150 (MQ=255)                                                                                         aGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCa                                                          >  1:452006/1‑150 (MQ=255)                                                                                                    acaacGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGaa                                              <  2:373013/151‑1 (MQ=255)                                                                                                       acGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGc                                           >  2:521943/1‑151 (MQ=255)                                                                                                              accGGAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGat                                     >  1:352421/1‑150 (MQ=255)                                                                                                                 ggAGCTGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCg                                 <  1:691246/151‑1 (MQ=255)                                                                                                                      tGAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGCGCAGGAAGGCGTTGATATCGCCAt                             >  1:429016/1‑150 (MQ=255)                                                                                                                       gAACGATATGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATc                            <  2:805948/150‑1 (MQ=255)                                                                                                                            atatGTGCCGCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAAc                      <  1:382755/151‑1 (MQ=255)                                                                                                                                     gCATCATCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGAtt             >  1:500742/1‑151 (MQ=255)                                                                                                                                      catcatCAGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTg            >  2:211082/1‑151 (MQ=255)                                                                                                                                        tcatcaGCCATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTc          >  1:853048/1‑151 (MQ=255)                                                                                                                                                cATGCCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCGCTATCgg  <  1:754743/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   ACAGCAGCAACCCAGGCTTCCTTGTGGAAGATTGCCGGGTACGAGTACGTCTGAATAACCAAAGCTGGGTGTTAAACATCGACAGCAAAGGTCAGTTTAACAACGTACCACCGGAGCTGAACGATATGTGCCGCATCATCAGCCATGTCCACCAGCATCATCATTATCTGCTGGGCCGCGTGGAGGTGTAATCATGTTGATTCTGAAGAATGTCACTGCGGTACAGCTACACCCGGCAAAAGTGCAGGAAGGCGTTGATATCGCCATCGAAAACGATGTGATTGTCGCTATCGG  >  NC_000913/3018968‑3019261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑