Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,039,247	A→G	V166A (GTG→GCG)	xerD ←	site‑specific tyrosine recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,039,247	0	A	G	100.0%	164.6 / NA	47	V166A (GTG→GCG)	xerD	site‑specific tyrosine recombinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/25);  total (22/25)

CGCACGCTGGCTGGGAAACAACACGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGTTCGAC  >  NC_000913/3039099‑3039392                                                                                                                                                     |                                                                                                                                                  cgcACGCTGGCTGGGAAACAACACGTCAATTGACACACCATTCAGCAGCCCCGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACcgcg                                                                                                                                                 <  2:316320/151‑1 (MQ=255) cgcACGCTGGCTGGGAAACAACACGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACcgcg                                                                                                                                                 >  2:600547/1‑151 (MQ=255)            tggGAAACAACACGTCAATTGACACACCATTTAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGc                                                                                                                                        >  1:325856/1‑149 (MQ=255)             gggAAACAACACGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAg                                                                                                                                      <  2:15424/150‑1 (MQ=255)                  acaacaCGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGat                                                                                                                                >  2:539875/1‑151 (MQ=255)                      cacGTCAATTGCCACACCATTCAGCACCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATc                                                                                                                             <  1:729283/150‑1 (MQ=255)                        cGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACt                                                                                                                          >  1:297147/1‑151 (MQ=255)                            aaTTGACACCCCATCCAGCAGCCCCGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCAtt                                                                                                                      <  1:711811/151‑1 (MQ=255)                            aaTTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCAc                                                                                                                           <  1:116416/146‑1 (MQ=255)                              ttGACACACCATTCAGCAGCCACGGCCGCCCATGTTCCAAATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTg                                                                                                                     <  2:641891/150‑1 (MQ=255)                                gACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTc                                                                                                                   >  1:835987/1‑150 (MQ=255)                                            agcagcCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGtt                                                                                                      >  1:200573/1‑151 (MQ=255)                                              cagcCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTc                                                                                                     >  1:199871/1‑150 (MQ=255)                                                 ccccGGACGCCCATGTTCCAGATGGGTTCCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCa                                                                                                    <  1:64797/145‑1 (MQ=255)                                                  cACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAgaga                                                                                                <  2:267831/151‑1 (MQ=255)                                                       aCGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGc                                                                                           >  1:740640/1‑151 (MQ=255)                                                       aCGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGc                                                                                           >  1:377906/1‑151 (MQ=255)                                                         gCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGc                                                                                           >  1:708611/1‑149 (MQ=255)                                                          cccATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGc                                                                                        <  2:207296/151‑1 (MQ=255)                                                          cccATGTTCCAGAAAGGTTCCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAg                                                                                         <  1:850734/150‑1 (MQ=255)                                                           ccATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGcc                                                                                       >  2:683459/1‑151 (MQ=255)                                                            cATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGccc                                                                                      >  2:529703/1‑151 (MQ=255)                                                                   cAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGc                                                                                >  1:444866/1‑150 (MQ=255)                                                                     gATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGGCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCAt                                                                              >  1:373628/1‑150 (MQ=255)                                                                         ggTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATacaa                                                                          <  2:574016/150‑1 (MQ=255)                                                                                  ccagTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGc                                                                >  1:313303/1‑151 (MQ=255)                                                                                   cagTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGc                                                                >  1:110585/1‑150 (MQ=255)                                                                                                ctTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATcg                                                    <  2:533647/149‑1 (MQ=255)                                                                                                   cACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTa                                                <  2:720739/150‑1 (MQ=255)                                                                                                   cACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTa                                                <  2:186591/150‑1 (MQ=255)                                                                                                        aaCGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCa                                          <  2:848120/151‑1 (MQ=255)                                                                                                          cGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGt                                        <  2:377930/151‑1 (MQ=255)                                                                                                          cGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGt                                        <  2:740683/151‑1 (MQ=255)                                                                                                           ggCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTg                                       <  2:326752/151‑1 (MQ=255)                                                                                                                 aGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGa                                  >  1:761274/1‑150 (MQ=255)                                                                                                                 aGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGAt                                 >  2:622150/1‑151 (MQ=255)                                                                                                                   aCGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCg                               <  1:539838/151‑1 (MQ=255)                                                                                                                    cGCTCTTTGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCg                               >  1:685856/1‑150 (MQ=255)                                                                                                                         tttGTTGCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAAt                         >  2:827574/1‑151 (MQ=255)                                                                                                                               gCCTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGcc                   >  1:696824/1‑151 (MQ=255)                                                                                                                                ccTTTACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCt                  <  1:318150/151‑1 (MQ=255)                                                                                                                                    tACCAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGta               <  1:767255/150‑1 (MQ=255)                                                                                                                                      ccAATGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGtaata            <  1:363384/151‑1 (MQ=255)                                                                                                                                         aTGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAAc         <  1:286091/151‑1 (MQ=255)                                                                                                                                         aTGACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAAc         <  1:217134/151‑1 (MQ=255)                                                                                                                                           gACCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGt       >  2:240312/1‑151 (MQ=255)                                                                                                                                            aCCCGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGtt      <  1:160768/151‑1 (MQ=255)                                                                                                                                              ccGTACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGTTCg    >  1:129452/1‑151 (MQ=255)                                                                                                                                                 tACCGCGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGTTCGAc  <  2:575823/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CGCACGCTGGCTGGGAAACAACACGTCAATTGACACACCATTCAGCAGCCACGGACGCCCATGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCATACAACACTTCAAGCATGGCTTTATCGCGTAGCTCCAGTGGCTGATCGATTAATGGTGCCTGTAATAAACGTTCGAC  >  NC_000913/3039099‑3039392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑