Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,040,408	C→T	intergenic (+32/+8)	fldB → / ← cptA	flavodoxin 2/toxin of CptAB toxin‑antitoxin pair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,040,408	0	C	T	97.7%	143.6 / ‑3.9	44	intergenic (+32/+8)	fldB/cptA	flavodoxin 2/toxin of CptAB toxin‑antitoxin pair
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (21/21);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

GGGCAACTGTTCGTGGGTCTGGCGCTGGATGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCCGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTA  >  NC_000913/3040260‑3040551                                                                                                                                                     |                                                                                                                                                gggCAACTGTTCGTGGGTCTGGCGCTGGATGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGt                                                                                                                                               >  1:259067/1‑151 (MQ=255)         gTTCGTGGGTCTGGCGCTGGATGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGAGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCatctt                                                                                                                                         >  2:588010/1‑149 (MQ=255)           tCGTGGGTCTGGCGCTGGATGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCatctttat                                                                                                                                      <  2:634643/150‑1 (MQ=255)            cGTGGGTCTGGCGCTGGATGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCatctttatct                                                                                                                                    <  1:568433/151‑1 (MQ=255)               gggTCTGGCGCTGGATGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTg                                                                                                                                 <  2:472977/151‑1 (MQ=255)                   cTGGCGCTGGATGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGtc                                                                                                                             >  2:289713/1‑151 (MQ=255)                       cgcTGGATGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCtt                                                                                                                         <  1:757759/151‑1 (MQ=255)                           ggATGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCttgt                                                                                                                       <  1:205705/149‑1 (MQ=255)                             aTGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGca                                                                                                                   <  1:461052/151‑1 (MQ=255)                              tGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCCGTCATCTTTATCTTTGCGTCTCTTGTTGca                                                                                                                   >  2:39007/1‑150 (MQ=255)                               gAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGcaa                                                                                                                  <  1:633845/150‑1 (MQ=255)                                  aCTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGcaacaa                                                                                                               >  2:595026/1‑150 (MQ=255)                                              gcccTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGTTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTaaa                                                                                                  <  1:367054/149‑1 (MQ=255)                                              gACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTAGCGTCTCTTGTTGCAACAAAATCCGCCGTaa                                                                                                   >  2:633039/1‑150 (MQ=255)                                              gACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCGTAACGAAAAGGCAGAGCATTACGCCTGATCTCCCTGACGGATGAGCGCAAATGCTTTGCGGTAATCTTTACCTGTGCGCCTCTTGTTCCAACAATAAccgttgtga                                                                                                   <  1:323483/150‑7 (MQ=255)                                                   tAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATccc                                                                                              >  1:297795/1‑150 (MQ=255)                                                   tAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATcc                                                                                               <  2:94858/149‑1 (MQ=255)                                                        acgaGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCAtt                                                                                        >  2:759602/1‑151 (MQ=255)                                                          gaGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTc                                                                                       >  1:682176/1‑150 (MQ=255)                                                            gCGTATTCAGAGCTGGTGCGAGCAAATCCCCACCGAAATGGCAGAGCATTCCGCCTGATCTCACTGACGGCTTAGCGCTTATGCTTTGCTGTCATCTTTATCTTTGCGTCTTTTTTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAg                                                                                     <  2:2905/150‑1 (MQ=255)                                                              gTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCtt                                                                                  >  2:652729/1‑151 (MQ=255)                                                                aTTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGAATATGCTTTGCTGTCATCTTTATCTTGGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTc                                                                                 <  2:383752/150‑1 (MQ=255)                                                                 ttCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGt                                                                               >  1:752950/1‑151 (MQ=255)                                                                 ttCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTGGCGTCTCTTTTTGCAACAAAACCCGCCGTAAATCCCGCCATTCAGCTTc                                                                                 <  1:558727/149‑1 (MQ=255)                                                                        cTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATg                                                                          <  2:452711/149‑1 (MQ=255)                                                                           gTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTc                                                                     <  2:823327/151‑1 (MQ=255)                                                                               gAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCt                                                                 >  2:552627/1‑151 (MQ=255)                                                                                   aaaTCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCAGCTgcca                                                             <  2:687856/151‑1 (MQ=255)                                                                                       ccTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTgccagcca                                                         <  2:747320/151‑1 (MQ=255)                                                                                           aaCGAAATGGCAGAGCATTCCGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATaa                                                      <  2:639808/150‑1 (MQ=255)                                                                                           aaCGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATaa                                                      >  2:437290/1‑150 (MQ=255)                                                                                              gAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGt                                                  >  1:666060/1‑151 (MQ=255)                                                                                               aaaTGGCAGAGCTTTCCCCCTGTTCTCACTGACGGGTTAGCCCAAATGCTTTCCTGTCATCTTTATCTTGGCGTCTCTTGTTGCAACAAAACCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATg                                                   <  1:663657/149‑1 (MQ=255)                                                                                               aaaTGGCAGAGCATTACGCCTGATCCCCCTGACGGCTTAGCGCATACGCTTTGCTGTCATCTTTATTTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGtt                                                 <  1:388069/151‑1 (MQ=255)                                                                                                 aTGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGc                                               >  2:752201/1‑151 (MQ=255)                                                                                                      agagCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGttt                                          >  1:457514/1‑151 (MQ=255)                                                                                                      agagCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGttt                                          >  2:413202/1‑151 (MQ=255)                                                                                                               cGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCa                                   <  2:339543/149‑1 (MQ=255)                                                                                                                 ccTGATCTCACTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTGGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAg                               <  1:308492/151‑1 (MQ=255)                                                                                                                           cTGACGGCTTAGCGCATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACg                     >  1:506383/1‑151 (MQ=255)                                                                                                                                         cATATGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACGCAGCATCATGCCGc       >  1:832601/1‑151 (MQ=255)                                                                                                                                          atatGCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACGCAGCATCATGCCGct      >  1:15284/1‑151 (MQ=255)                                                                                                                                              gCTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCtt   >  1:218679/1‑150 (MQ=255)                                                                                                                                               cTTTGCTGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTa  >  1:255774/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                GGGCAACTGTTCGTGGGTCTGGCGCTGGATGAAACTAACCAGTATGACCTTAGCGACGAGCGTATTCAGAGCTGGTGCGAGCAAATCCTCAACGAAATGGCAGAGCATTACGCCTGATCTCACTGACGGCTTAGCGCATATGCTTTGCCGTCATCTTTATCTTTGCGTCTCTTGTTGCAACAAAATCCGCCGTAAATCCCGCCATTCAGCTTCGTCCATGCTGTCGGCTGCCAGCCATAAATGTTGCCGTTTACCGCCATCAGAACGTAAACGCAGCATCATGCCGCTCTTA  >  NC_000913/3040260‑3040551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑