Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,108,362 G→A A265V (GCG→GTG)  speC ← ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,108,3620GA97.8% 146.5 / ‑4.4 47A265V (GCG→GTG) speCornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (23/22);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

GACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGACTTA  >  NC_000913/3108217‑3108508
                                                                                                                                                 |                                                                                                                                                  
gACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACg                                                                                                                                               <  2:164751/151‑1 (MQ=255)
gACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACg                                                                                                                                               >  1:66806/1‑151 (MQ=255)
    cACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTcgc                                                                                                                                           <  2:592660/151‑1 (MQ=255)
      cgcgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGGCGGCTTTTTCTGGCACAACGTcgcg                                                                                                                                          >  1:663034/1‑150 (MQ=255)
        cgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAt                                                                                                                                       <  2:48889/151‑1 (MQ=255)
              cAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTg                                                                                                                                 >  1:703079/1‑151 (MQ=255)
               aaaCAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTgg                                                                                                                                <  2:69276/151‑1 (MQ=255)
                aaCAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGgc                                                                                                                               >  2:712003/1‑151 (MQ=255)
                aaCAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGgc                                                                                                                               >  2:321602/1‑151 (MQ=255)
                 aCAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGgcg                                                                                                                              >  1:639732/1‑151 (MQ=255)
                  cAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACATCGCGAATTTGCTGgcgc                                                                                                                             <  1:637581/151‑1 (MQ=255)
                    gAATGTAATCACACAGATGCCCAACGGGATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCACCATGCCGCGAATTTGCTGgcg                                                                                                                              >  1:450457/1‑148 (MQ=255)
                      aTGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGAt                                                                                                                         <  2:188837/151‑1 (MQ=255)
                        gTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATa                                                                                                                        >  1:454774/1‑150 (MQ=255)
                           aTCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCACGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATActct                                                                                                                    >  1:108579/1‑151 (MQ=255)
                             cacacaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTc                                                                                                                  >  2:415816/1‑151 (MQ=255)
                             cacacaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTc                                                                                                                  <  1:538905/151‑1 (MQ=255)
                                acaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAtt                                                                                                               <  2:79743/151‑1 (MQ=255)
                                        ccAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAg                                                                                                        >  2:607577/1‑150 (MQ=255)
                                         cAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATGTGCTGGCGCAGATACTCTTCATTAAAACAGTg                                                                                                      <  1:426289/151‑1 (MQ=255)
                                           aCGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTgcg                                                                                                    >  2:583161/1‑151 (MQ=255)
                                                aTCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATc                                                                                                >  2:1612/1‑150 (MQ=255)
                                                   gATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATa                                                                                            <  2:433342/151‑1 (MQ=255)
                                                        ccTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGcc                                                                                       <  2:57259/151‑1 (MQ=255)
                                                                gCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGaaa                                                                                >  1:193144/1‑150 (MQ=255)
                                                                     ataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGGCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAAc                                                                          >  1:404227/1‑151 (MQ=255)
                                                                       aGACAGTGCCGTCATGGGTTCCCAGCTAAATAAGCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAAATCCTCTTCATTAAAACAGTGCGCATAAATACCGCCAATGAAACCAAACgg                                                                        <  1:216442/151‑1 (MQ=255)
                                                                         aCAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATCCTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACgg                                                                        <  2:13853/149‑1 (MQ=255)
                                                                         aCAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGt                                                                      >  2:586362/1‑151 (MQ=255)
                                                                                 gTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACACCGTCGCGAATTTGCGGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGaa                                                              <  2:119420/151‑1 (MQ=255)
                                                                                       ggTTCCCAGCTAAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATAAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTc                                                         <  1:415784/150‑1 (MQ=255)
                                                                                           cccAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGAt                                                    <  2:574656/151‑1 (MQ=255)
                                                                                           cccAGCTAAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGAt                                                    <  2:435942/151‑1 (MQ=255)
                                                                                            ccAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGAt                                                    >  1:457348/1‑150 (MQ=255)
                                                                                                    ataatCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCg                                           >  2:61735/1‑151 (MQ=255)
                                                                                                            cAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGccccc                                   >  1:410442/1‑151 (MQ=255)
                                                                                                                  aaaCGGGCGCGGCAGGGCGGTTTTTTCTGGCACAACGTCGCGAATTTGCGGGCGCGGATACCCTTCATTAAAACAGTCCGCATAAACACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCt                               <  1:261282/147‑1 (MQ=255)
                                                                                                                    aCGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAAt                           >  1:795027/1‑151 (MQ=255)
                                                                                                                      gggCGCGGCGGGCGGTCTTTTTCTGGCACACGGCCGGAAATTTCCGGACGCAAATCCTCTCCTTAAAAAAAGCCCGCATAAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGCCCGGCGTCGCCCCCGCCTGAATc                          <  1:673488/150‑1 (MQ=255)
                                                                                                                          gcgGCAGGTCGTTTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATCCTCTTCATAAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAgcg                      <  2:675112/150‑1 (MQ=255)
                                                                                                                            ggCAGGTCGGTTTTTCCTGACCCCCCGGCGCGAATTTGCGGGCGTAGATACTCTTCATAAAACCAGAGCGCAAAAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAgcgcg                    <  2:467982/150‑1 (MQ=255)
                                                                                                                             gCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGcc                  >  2:298831/1‑151 (MQ=255)
                                                                                                                               aGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGACACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGcc                  >  1:505349/1‑149 (MQ=255)
                                                                                                                                   cGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGtgatg            >  1:716110/1‑151 (MQ=255)
                                                                                                                                             tGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGACtt   >  2:106873/1‑150 (MQ=255)
                                                                                                                                              ggCACACCGGCGCGAATTTGCTGGCGCAAATCCTCTTCATAAAAACAGTGCGCATAAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGACt    <  2:541338/148‑1 (MQ=255)
                                                                                                                                              ggCACAACGTCGCGAATTGGCGGGCGCAGATACTCTTCATAAAAACAGTGCGCATAAATCCCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGACTTa  <  1:57958/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGACTTA  >  NC_000913/3108217‑3108508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑