Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	396,691	T→C	V18A (GTT→GCT)	sbmA →	peptide antibiotic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	396,691	0	T	C	100.0%	188.5 / NA	53	V18A (GTT→GCT)	sbmA	peptide antibiotic transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

GGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTG  >  NC_000913/396551‑396838                                                                                                                                             |                                                                                                                                                    ggCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCattg                                                                                                                                           <  2:208889/151‑1 (MQ=255)     gTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCattgattg                                                                                                                                       <  2:169832/151‑1 (MQ=255)     gTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCattgattg                                                                                                                                       <  2:413577/151‑1 (MQ=255)     gTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCattgattg                                                                                                                                       <  1:858746/151‑1 (MQ=255)       ttCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGcc                                                                                                                                     >  2:786365/1‑151 (MQ=255)         cATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGt                                                                                                                                   >  1:682369/1‑151 (MQ=255)          aTTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGtt                                                                                                                                  <  2:615726/151‑1 (MQ=255)                        ttGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGc                                                                                                                    >  1:295289/1‑151 (MQ=255)                          gTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCg                                                                                                                  >  1:27975/1‑151 (MQ=255)                             gCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTg                                                                                                               <  1:189814/151‑1 (MQ=255)                              cGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTgg                                                                                                              <  2:400895/151‑1 (MQ=255)                                         ttAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTggg                                                                                                   >  2:389646/1‑151 (MQ=255)                                           aaTACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGt                                                                                                  <  1:529135/150‑1 (MQ=255)                                            aTACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCTGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTgg                                                                                                <  2:711589/151‑1 (MQ=255)                                               cacaGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGgcgc                                                                                             >  2:156655/1‑151 (MQ=255)                                                     agTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATc                                                                                        >  1:582193/1‑150 (MQ=255)                                                            aaaaCGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGGCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGgcg                                                                                 <  1:48168/150‑1 (MQ=255)                                                              aaCGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGTCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCtt                                                                              >  1:95738/1‑151 (MQ=255)                                                                        agTTAGCAGGAGTGCATATGCTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGc                                                                        >  1:568672/1‑147 (MQ=255)                                                                              cAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGAt                                                              >  2:575024/1‑151 (MQ=255)                                                                               aGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGAtt                                                             <  1:389614/151‑1 (MQ=255)                                                                                  aGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAgc                                                          <  1:758979/151‑1 (MQ=255)                                                                                   gTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAgcg                                                         >  1:318637/1‑151 (MQ=255)                                                                                    tGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAgcgc                                                        >  1:396336/1‑151 (MQ=255)                                                                                    tGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAgcgc                                                        >  2:829820/1‑151 (MQ=255)                                                                                           gTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGt                                                 <  2:300724/151‑1 (MQ=255)                                                                                             ttAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGttt                                               >  2:107667/1‑151 (MQ=255)                                                                                                  tCTTTTTTCCCAAAGCCGGCAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTTGCGCCGCGCGTTTCTgg                                           >  1:278406/1‑150 (MQ=255)                                                                                                   cTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTc                                         >  1:479776/1‑151 (MQ=255)                                                                                                    ttttttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCg                                        >  1:794833/1‑151 (MQ=255)                                                                                                     tttttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGt                                          <  1:272920/148‑1 (MQ=255)                                                                                                     tttttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCg                                        <  2:104782/150‑1 (MQ=255)                                                                                                       tttCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGtt                                      <  2:319738/150‑1 (MQ=255)                                                                                                         tCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCg                                        >  2:439837/1‑146 (MQ=255)                                                                                                         tCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCg                                        <  1:439803/146‑1 (MQ=255)                                                                                                           ccAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGAtt                                 <  2:34158/151‑1 (MQ=255)                                                                                                            cAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGAttt                                <  1:780734/151‑1 (MQ=255)                                                                                                                gCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTg                            >  2:851356/1‑151 (MQ=255)                                                                                                                gCCGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTg                            <  2:219848/151‑1 (MQ=255)                                                                                                                  cGGGAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAt                          <  1:477741/151‑1 (MQ=255)                                                                                                                    ggAACGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAttt                        <  2:685964/151‑1 (MQ=255)                                                                                                                     gAACTTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAtt                         <  2:202973/149‑1 (MQ=255)                                                                                                                      aaCGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAttttt                      >  2:591823/1‑151 (MQ=255)                                                                                                                        cGTTTTTTCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAtttttt                     >  2:768496/1‑150 (MQ=255)                                                                                                                          ttttttCTCTCGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACg                  >  2:634657/1‑151 (MQ=255)                                                                                                                                ctctcGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTtact            >  1:841668/1‑151 (MQ=255)                                                                                                                                ctctcGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTta              >  1:53310/1‑149 (MQ=255)                                                                                                                                 tctcGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTtact            >  2:117068/1‑150 (MQ=255)                                                                                                                                  ctcGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTtac             <  1:786309/148‑1 (MQ=255)                                                                                                                                    cGGCCTTCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACa         <  2:591368/150‑1 (MQ=255)                                                                                                                                         ttCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGttt   >  1:234546/1‑151 (MQ=255)                                                                                                                                          tCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGttt   <  1:429440/150‑1 (MQ=255)                                                                                                                                          tCGCTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTg  >  1:755642/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                                    GGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTG  >  NC_000913/396551‑396838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑