Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,118,599	C→T	V172I (GTT→ATT)	sslE ←	putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,118,599	0	C	T	100.0%	174.9 / NA	50	V172I (GTT→ATT)	sslE	putative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/19);  total (31/19)

CATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAAC  >  NC_000913/3118452‑3118746                                                                                                                                                    |                                                                                                                                                    cATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCg                                                                                                                                                   <  1:844070/150‑1 (MQ=255)    tGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCAtt                                                                                                                                               >  2:21638/1‑151 (MQ=255)      aCCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGc                                                                                                                                             <  2:259410/151‑1 (MQ=255)        cAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCtt                                                                                                                                           <  1:573459/151‑1 (MQ=255)            ttttgGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGATAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTc                                                                                                                                        >  1:618233/1‑150 (MQ=255)             ttttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTc                                                                                                                                        <  2:231461/149‑1 (MQ=255)               ttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGt                                                                                                                                    >  2:567309/1‑151 (MQ=255)                 gAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTc                                                                                                                                   <  2:149208/150‑1 (MQ=255)                   aCTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATc                                                                                                                                <  2:298964/151‑1 (MQ=255)                     tctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCgg                                                                                                                              >  2:805379/1‑151 (MQ=255)                         ccGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGc                                                                                                                           <  2:576768/150‑1 (MQ=255)                            gTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGc                                                                                                                       >  2:608118/1‑151 (MQ=255)                                aaGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGt                                                                                                                   >  1:741982/2‑151 (MQ=255)                                  gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTc                                                                                                                 <  2:386771/151‑1 (MQ=255)                                      gATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGACCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgc                                                                                                             >  1:551898/1‑151 (MQ=255)                                       aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                                                                                                            >  1:484053/1‑151 (MQ=255)                                         ttGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                                                                                                           <  2:394517/150‑1 (MQ=255)                                           gCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGAATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGt                                                                                                         <  2:337880/150‑1 (MQ=255)                                                 aCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCaa                                                                                                   <  2:733463/150‑1 (MQ=255)                                                  cAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAgg                                                                                                 >  2:598301/1‑151 (MQ=255)                                                     cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCt                                                                                               >  2:855933/1‑150 (MQ=255)                                                            aaGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGaca                                                                                       <  2:657186/151‑1 (MQ=255)                                                               cGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACtt                                                                                    >  2:794029/1‑151 (MQ=255)                                                                gTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACttt                                                                                   >  2:432274/1‑151 (MQ=255)                                                                   tACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACttttt                                                                                 <  2:71808/150‑1 (MQ=255)                                                                         cGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAc                                                                             <  1:491085/148‑1 (MQ=255)                                                                               ccGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCaa                                                                    <  2:467973/151‑1 (MQ=255)                                                                                         cGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACgcgc                                                           >  1:14702/1‑150 (MQ=255)                                                                                          gtaaaaCAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgc                                                             >  1:300130/6‑147 (MQ=255)                                                                                           tCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGc                                                        >  2:373109/1‑151 (MQ=255)                                                                                           tCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGc                                                        >  2:465733/1‑151 (MQ=255)                                                                                            cAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCt                                                       >  2:148467/1‑151 (MQ=255)                                                                                                 aaaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctg                                                   >  2:64229/1‑150 (MQ=255)                                                                                                 aaaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctg                                                   >  2:663290/1‑150 (MQ=255)                                                                                                  aaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgac                                                 >  2:671735/1‑151 (MQ=255)                                                                                                        ttCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAgtg                                           >  1:511/1‑151 (MQ=255)                                                                                                        ttCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAg                                             <  1:362454/149‑1 (MQ=255)                                                                                                           tGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTg                                        >  2:647150/1‑151 (MQ=255)                                                                                                             tATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGa                                       >  1:50980/1‑150 (MQ=255)                                                                                                               ttCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAgg                                    >  2:445894/1‑151 (MQ=255)                                                                                                               ttCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAgg                                    >  2:65049/1‑151 (MQ=255)                                                                                                                tCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGt                                   >  2:342955/1‑151 (MQ=255)                                                                                                                  gCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTgg                                 >  1:295544/1‑151 (MQ=255)                                                                                                                   ccGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGc                                >  2:39289/1‑151 (MQ=255)                                                                                                                      gACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAAt                             <  1:360928/151‑1 (MQ=255)                                                                                                                            tGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTTAAGGTGGCAATTGTCgt                       >  1:26267/1‑151 (MQ=255)                                                                                                                                              gCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt     >  1:668275/1‑151 (MQ=255)                                                                                                                                               cGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt     <  2:187397/150‑1 (MQ=255)                                                                                                                                               cGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt     <  2:483514/150‑1 (MQ=255)                                                                                                                                                 aaaTCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAAc  >  2:561678/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAAC  >  NC_000913/3118452‑3118746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑