Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,227,104	G→A	V291M (GTG→ATG)	ygjI →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,227,104	0	G	A	100.0%	174.2 / NA	49	V291M (GTG→ATG)	ygjI	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/27);  total (22/27)

GTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAG  >  NC_000913/3226962‑3227242                                                                                                                                               |                                                                                                                                           gTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCtt                                                                                                                                     <  2:36383/150‑1 (MQ=255)       cGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACcgcg                                                                                                                              >  1:150899/1‑151 (MQ=255)       cGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACcgcg                                                                                                                              >  1:217363/1‑151 (MQ=255)            cctcGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGt                                                                                                                          <  2:372099/150‑1 (MQ=255)                   gCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTcc                                                                                                                  <  2:590304/151‑1 (MQ=255)                       aTTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCctgc                                                                                                              <  2:733599/151‑1 (MQ=255)                           aCGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGAATTCCACGGCATGGCGGCGTATTTTGGTCTAACGGAAGCGTTGATGAATCGCTTTGCCGGTCTGATGTCCTTTACCGCGATGTTCGGTTTCCTGCTGAt                                                                                                          >  2:769564/1‑151 (MQ=255)                              tCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGAtgtg                                                                                                       >  2:754019/1‑151 (MQ=255)                                  cGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGAcc                                                                                                   <  1:507768/151‑1 (MQ=255)                                     cagcagCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGc                                                                                                 <  2:852733/150‑1 (MQ=255)                                      agcagcAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTATGATTACCTTTACCGCGATGTTCGGGTCCCTGCTGCTGTGGACCGCa                                                                                                >  2:628621/1‑150 (MQ=255)                                               gagTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGa                                                                                      <  1:500799/151‑1 (MQ=255)                                                    gAAATTTACCGGCGGATCGGTGCAGGAATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAAtt                                                                                 >  2:439973/1‑151 (MQ=255)                                                      aaTTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAAtttt                                                                               >  1:62607/1‑151 (MQ=255)                                                         ttACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTttct                                                                             <  2:750800/150‑1 (MQ=255)                                                              ggcggATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCg                                                                       <  1:687927/151‑1 (MQ=255)                                                                      ggTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCg                                                               >  1:626990/1‑151 (MQ=255)                                                                      ggTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCg                                                               <  1:855711/151‑1 (MQ=255)                                                                        tGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCgg                                                              >  2:755936/1‑150 (MQ=255)                                                                              tATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCAt                                                       >  2:26981/1‑151 (MQ=255)                                                                                 tCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCtt                                                    >  1:115018/1‑151 (MQ=255)                                                                                       gCATGGCGGCGTATTTTAGTCTACCGGAAGCATTGACGAATCGCTTTATCGCTCCAATGTCCTTTTCCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCACCGACAGTGAAAATTTTCTGCTCCGAAATCCCGGTAGGCATCTTAGGTa                                               <  1:235145/150‑1 (MQ=255)                                                                                         aTGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTa                                               >  2:627688/1‑148 (MQ=255)                                                                                         aTGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGa                                            <  2:568318/151‑1 (MQ=255)                                                                                            gcggcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGaaaa                                         <  2:150922/151‑1 (MQ=255)                                                                                            gcggcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGaaa                                          >  1:458544/1‑150 (MQ=255)                                                                                             cggcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAAc                                        <  2:738652/151‑1 (MQ=255)                                                                                              ggcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAAcc                                       <  2:796679/151‑1 (MQ=255)                                                                                               gcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCg                                      <  1:155831/151‑1 (MQ=255)                                                                                                cgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGTTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGt                                     >  2:435569/1‑151 (MQ=255)                                                                                                cgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGt                                     <  2:579764/151‑1 (MQ=255)                                                                                                     tttGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAAc                                >  1:14687/1‑151 (MQ=255)                                                                                                         gTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGaa                            <  2:728561/151‑1 (MQ=255)                                                                                                         gTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGa                             >  2:655745/1‑150 (MQ=255)                                                                                                             aCCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACg                          <  2:707725/149‑1 (MQ=255)                                                                                                             aCCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGa                         <  2:559835/150‑1 (MQ=255)                                                                                                              ccGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACAGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTTAACGaa                        >  1:648780/1‑150 (MQ=255)                                                                                                               cGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGaaaa                      <  1:97170/151‑1 (MQ=255)                                                                                                                  aaGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACgg                   >  1:585044/1‑151 (MQ=255)                                                                                                                       ttGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTc              >  1:33013/1‑151 (MQ=255)                                                                                                                        tgatgaATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTc              <  1:608855/150‑1 (MQ=255)                                                                                                                          atgaATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCgg           <  2:694785/151‑1 (MQ=255)                                                                                                                          atgaATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCgg           >  1:489354/1‑151 (MQ=255)                                                                                                                             aaTCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgc        <  2:190164/151‑1 (MQ=255)                                                                                                                             aaTCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcg         >  1:135383/1‑150 (MQ=255)                                                                                                                              aTCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgcg       <  1:810417/151‑1 (MQ=255)                                                                                                                                cGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgcgcg     >  1:666565/1‑151 (MQ=255)                                                                                                                                  cTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgcgcgc    <  2:338624/150‑1 (MQ=255)                                                                                                                                    ttGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAg  <  2:458580/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                           GTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAG  >  NC_000913/3226962‑3227242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑