Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,236,954 G→A A139T (GCC→ACC)  ygjQ → DUF218 superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,236,9540GA98.4% 241.8 / NA 64A139T (GCC→ACC) ygjQDUF218 superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (36/27);  minor base C (0/1);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTGCCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAATATGAAGCACGGTTTTTATACTC  >  NC_000913/3236809‑3237101
                                                                                                                                                 |                                                                                                                                                   
aTGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCaaa                                                                                                                                                <  1:212326/151‑1 (MQ=255)
  ggCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCaaaa                                                                                                                                               >  2:246814/1‑150 (MQ=255)
  ggCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCaaaa                                                                                                                                               <  2:402290/150‑1 (MQ=255)
   gctgctGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCaaaa                                                                                                                                               >  2:463814/1‑149 (MQ=255)
   gctgctGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAg                                                                                                                                             <  1:51977/151‑1 (MQ=255)
         ggTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGttt                                                                                                                                         <  1:628380/149‑1 (MQ=255)
           tAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTgg                                                                                                                                     >  2:215257/1‑151 (MQ=255)
             aGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTg                                                                                                                                   >  2:255129/1‑151 (MQ=255)
                 gCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGaaaa                                                                                                                               <  2:399242/151‑1 (MQ=255)
                    aTAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAAcc                                                                                                                             >  1:141139/1‑150 (MQ=255)
                       aCGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCatat                                                                                                                         <  1:399593/151‑1 (MQ=255)
                             gTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCAt                                                                                                                   >  1:386235/1‑151 (MQ=255)
                               aaaaaTTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCAtt                                                                                                                  <  2:56522/150‑1 (MQ=255)
                                 aaaTTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTAtc                                                                                                               <  2:666762/151‑1 (MQ=255)
                                    ttACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTAt                                                                                                                >  1:547467/1‑147 (MQ=255)
                                    ttACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTAt                                                                                                                <  2:547504/147‑1 (MQ=255)
                                    ttACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCg                                                                                                            >  1:671876/1‑151 (MQ=255)
                                     tACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCg                                                                                                            <  2:506859/150‑1 (MQ=255)
                                     tACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCg                                                                                                            <  1:451166/150‑1 (MQ=255)
                                       cGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATACCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCTCCATTATCTCGCaa                                                                                                         <  1:452176/151‑1 (MQ=255)
                                        gATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCa                                                                                                          >  1:504160/1‑149 (MQ=255)
                                             aGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAAttt                                                                                                   >  2:647249/1‑151 (MQ=255)
                                             aGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAAttt                                                                                                   <  1:212439/151‑1 (MQ=255)
                                             aGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAAttt                                                                                                   <  1:404821/151‑1 (MQ=255)
                                                aTCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCAt                                                                                                >  2:236761/1‑151 (MQ=255)
                                                  cAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATaa                                                                                              <  1:806015/151‑1 (MQ=255)
                                                   aGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATaa                                                                                              <  1:263802/150‑1 (MQ=255)
                                                     gTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATc                                                                                            >  1:70987/1‑150 (MQ=255)
                                                       aTGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAAc                                                                                         >  2:426966/1‑151 (MQ=255)
                                                                 cTTTGATCGCTAAAGGCGTGCCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATctg                                                                               >  1:292482/1‑151 (MQ=255)
                                                                  tttGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATctgg                                                                              >  2:295205/1‑151 (MQ=255)
                                                                   ttGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATctgg                                                                              <  2:302429/150‑1 (MQ=255)
                                                                    tGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATctggct                                                                            >  2:824899/1‑151 (MQ=255)
                                                                    tGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATctggct                                                                            >  2:654021/1‑151 (MQ=255)
                                                                     gATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATctggctg                                                                           >  1:81186/1‑151 (MQ=255)
                                                                     gATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATctggctg                                                                           <  1:286730/151‑1 (MQ=255)
                                                                                  gTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACg                                                              >  2:464333/1‑151 (MQ=255)
                                                                                  gTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACg                                                              <  2:502778/151‑1 (MQ=255)
                                                                                  gTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACg                                                              >  2:175398/1‑151 (MQ=255)
                                                                                    aCCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACgg                                                             <  1:477761/150‑1 (MQ=255)
                                                                                    aCCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGc                                                            >  2:137140/1‑151 (MQ=255)
                                                                                    aCCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGc                                                            >  2:402690/1‑151 (MQ=255)
                                                                                      cAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTa                                                                                                                 <  2:349949/96‑1 (MQ=255)
                                                                                      cAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTa                                                                                                                 >  1:349926/1‑96 (MQ=255)
                                                                                      cAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCAt                                                          >  2:40378/1‑151 (MQ=255)
                                                                                           aaaGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACg                                                     >  1:556547/1‑151 (MQ=255)
                                                                                               tGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTAt                                                 >  1:539698/1‑151 (MQ=255)
                                                                                                     tctGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATc                                                <  1:70950/146‑1 (MQ=255)
                                                                                                        gCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTa                                         >  1:426811/1‑150 (MQ=255)
                                                                                                         cGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAAc                                       >  2:466658/1‑151 (MQ=255)
                                                                                                             tATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACgcgc                                   >  2:827604/1‑151 (MQ=255)
                                                                                                              aTGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAAcgcg                                    <  2:810455/149‑1 (MQ=255)
                                                                                                                   ggATTCTCAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGAcc                             >  2:417827/1‑151 (MQ=255)
                                                                                                                       tctcAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCa                          >  2:412137/1‑150 (MQ=255)
                                                                                                                       tctcAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCa                          >  2:362115/1‑150 (MQ=255)
                                                                                                                        ctcAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAat                        >  2:138138/1‑151 (MQ=255)
                                                                                                                          cAACGCTGGATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAatat                      >  2:367793/1‑151 (MQ=255)
                                                                                                                            actcTGGATGCGTTTGTTCGTCTCAAAAAGGTTTTTGGTGAAAACCATACCACCATTATCACGCAAGAATTTCATAATCAACGGGCCAACTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCaa                         <  1:704963/143‑1 (MQ=255)
                                                                                                                                 ggATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAATATGAATCAc               <  1:8066/151‑1 (MQ=255)
                                                                                                                                 ggATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAATATGAAGCa                <  2:728333/150‑1 (MQ=255)
                                                                                                                                  gATTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAATATGAAGCACg              <  1:466622/151‑1 (MQ=255)
                                                                                                                                   aTTCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAATATGAAGCACgg             >  1:326585/1‑151 (MQ=255)
                                                                                                                                     tCGGTAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAATATGAAGCACGGt            <  2:372057/150‑1 (MQ=255)
                                                                                                                                         tAGTGCGTACCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAATATGAAGCACGGttttt        >  1:669949/1‑150 (MQ=255)
                                                                                                                                           gtgcgtacCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAATATGAAGCACGGTTTTtat      >  2:371906/1‑150 (MQ=255)
                                                                                                                                              cgtacCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAATATGAAGCACGGTTTTTATACTc  >  1:535025/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGTAGTGCGTGCCAAAAAGGTTTTTGGTGAAAACCATATCACCATTATCTCGCAAGAATTTCATAATCAACGCGCCATCTGGCTGGCAAAACAGTACGGCATCGACGCTATCGGTTTTAACGCGCCAGACCTCAATATGAAGCACGGTTTTTATACTC  >  NC_000913/3236809‑3237101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: