Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,246,757	C→T	R36C (CGC→TGC)	exuR →	hexuronate regulon transcriptional repressor; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,246,757	0	C	T	100.0%	242.3 / NA	65	R36C (CGC→TGC)	exuR	hexuronate regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/36);  total (29/36)

TGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTG  >  NC_000913/3246609‑3246901                                                                                                                                                     |                                                                                                                                                 tGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGc                                                                                                                                                >  2:641012/1‑151 (MQ=255)       aaCAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTAt                                                                                                                                           <  1:185871/150‑1 (MQ=255)         cAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGc                                                                                                                                        <  2:734319/151‑1 (MQ=255)         cAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGc                                                                                                                                        >  1:282271/1‑151 (MQ=255)         cAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGc                                                                                                                                        >  1:360834/1‑151 (MQ=255)          aaaTATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGcc                                                                                                                                       >  2:501041/1‑151 (MQ=255)          aaaTATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGcc                                                                                                                                       >  1:580667/1‑151 (MQ=255)           aaTATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCg                                                                                                                                      <  1:263626/151‑1 (MQ=255)              ataGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATg                                                                                                                                   >  2:758683/1‑151 (MQ=255)                     gCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAg                                                                                                                              <  1:61523/149‑1 (MQ=255)                        gTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGt                                                                                                                         <  2:739577/151‑1 (MQ=255)                          aTCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTc                                                                                                                        <  1:694250/150‑1 (MQ=255)                          aTCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCa                                                                                                                       <  2:613110/151‑1 (MQ=255)                             aTGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGcc                                                                                                                    <  2:374498/151‑1 (MQ=255)                              tGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCg                                                                                                                   >  2:475338/1‑151 (MQ=255)                                 cTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTAc                                                                                                                >  2:819742/1‑151 (MQ=255)                                  tGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACg                                                                                                               >  2:339523/1‑151 (MQ=255)                                   ggAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTAc                                                                                                                <  1:834402/149‑1 (MQ=255)                                   ggAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTAc                                                                                                                <  1:54718/149‑1 (MQ=255)                                      gcgcATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGt                                                                                                             >  1:741077/1‑149 (MQ=255)                                                aaaTCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGc                                                                                                 >  1:365182/1‑151 (MQ=255)                                                aaaTCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGc                                                                                                 <  1:333835/151‑1 (MQ=255)                                                  aTCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGcc                                                                                                >  2:391081/1‑150 (MQ=255)                                                   tCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCcat                                                                                              >  2:233254/1‑151 (MQ=255)                                                    cACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCcat                                                                                              <  1:820213/150‑1 (MQ=255)                                                        gAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCcatcatca                                                                                         <  1:9619/151‑1 (MQ=255)                                                        gAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCcatcat                                                                                           <  1:779069/149‑1 (MQ=255)                                                         aaCCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCcatcatcat                                                                                        >  2:75961/1‑151 (MQ=255)                                                           ccACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGc                                                                                      <  1:715133/151‑1 (MQ=255)                                                                ccGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGaa                                                                                 <  2:6828/151‑1 (MQ=255)                                                                 cGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGa                                                                                  >  2:759791/1‑149 (MQ=255)                                                                      gTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGaa                                                                           <  1:233230/151‑1 (MQ=255)                                                                           aacaacTTGCCGCTAACCTTAAAGAGCGCGTCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTCTTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCTTCATCATGCTGGAAGTTGAAGGCTa                                                                      <  1:305182/151‑1 (MQ=255)                                                                            acaacTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTAt                                                                     >  2:149015/1‑151 (MQ=255)                                                                            acaacTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTAt                                                                     <  2:809600/151‑1 (MQ=255)                                                                             caacTTGCCGCTGCCCTGAAAGAGCGCGTCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATg                                                                    <  2:79538/151‑1 (MQ=255)                                                                               acTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTc                                                                  >  2:595605/1‑151 (MQ=255)                                                                                  tGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCg                                                                 <  2:809108/149‑1 (MQ=255)                                                                                      gCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTAtt                                                                                                                                          >  2:413717/1‑72 (MQ=255)                                                                                      gCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTAtt                                                                                                                                          <  1:413685/72‑1 (MQ=255)                                                                                        tgcggttaaaGAGCGCATCGAACAGGGCGCCCATCTGGTGGGCGATAAACTGCCTGCAGACTGCTTTATTGCCGCTGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGCAGTTGAAGACTATGTCGAAGTGCg                                                          >  1:577525/8‑150 (MQ=255)                                                                                         gACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCg                                                          <  1:523010/149‑1 (MQ=255)                                                                                              gAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAg                                                     <  1:407720/149‑1 (MQ=255)                                                                                              gAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGt                                                   >  1:679814/1‑151 (MQ=255)                                                                                               aaaGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGtt                                                  <  1:795909/151‑1 (MQ=255)                                                                                                  gagCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTc                                                 >  2:646368/1‑149 (MQ=255)                                                                                                  gagCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAg                                               <  2:329164/151‑1 (MQ=255)                                                                                                    gcgcATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGt                                             <  2:577618/151‑1 (MQ=255)                                                                                                      gcATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTAt                                           >  1:301569/1‑151 (MQ=255)                                                                                                        aTCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTc                                         <  2:310361/151‑1 (MQ=255)                                                                                                                        aTCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAAcc                          >  2:45105/1‑150 (MQ=255)                                                                                                                        aTCTGGTGGGTGATAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAAc                           <  1:248225/149‑1 (MQ=255)                                                                                                                                    aTAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATca             <  2:816607/151‑1 (MQ=255)                                                                                                                                    aTAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATca             <  1:378425/151‑1 (MQ=255)                                                                                                                                    aTAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATc              >  2:843060/1‑150 (MQ=255)                                                                                                                                     tAAACTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATc              >  2:829991/1‑149 (MQ=255)                                                                                                                                      aaaCTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATcagc           <  2:335531/151‑1 (MQ=255)                                                                                                                                      aaaCTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATcagc           >  1:208419/1‑151 (MQ=255)                                                                                                                                        aCTGCCTGCAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATcagcag         <  2:732577/151‑1 (MQ=255)                                                                                                                                          tgcctgcAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAggc       >  1:487071/1‑151 (MQ=255)                                                                                                                                           gcctgcAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAgg        <  1:819686/149‑1 (MQ=255)                                                                                                                                             ctgcAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAggcggc    >  2:822526/1‑151 (MQ=255)                                                                                                                                               gcAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCt   <  2:694776/150‑1 (MQ=255)                                                                                                                                               gcAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCt   <  2:139236/150‑1 (MQ=255)                                                                                                                                                cAGAATGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTg  >  1:495387/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                 TGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTG  >  NC_000913/3246609‑3246901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑