Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,257,613	G→A	A133V (GCC→GTC)	yhaO ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,257,613	0	G	A	100.0%	215.8 / NA	58	A133V (GCC→GTC)	yhaO	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/32);  total (26/32)

AATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATC  >  NC_000913/3257471‑3257760                                                                                                                                               |                                                                                                                                                    aaTTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGa                                                                                                                                              >  1:787488/1‑150 (MQ=255)   ttttGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAAt                                                                                                                                            >  2:336681/1‑150 (MQ=255)       gAATAGCAATATCTAGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGAGCATAAAAGGGACTGTCTGAGAGCAACCCGTCCGTCACGCAGAAGGTATGCAGGTAGGAAGCACTAACGTTGGTGATGACGGTGGAATAGAc                                                                                                                                        <  1:168627/150‑1 (MQ=255)       gAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACg                                                                                                                                       >  1:512767/1‑151 (MQ=255)        aaTAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGa                                                                                                                                      <  1:106800/151‑1 (MQ=255)         aTAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGaa                                                                                                                                     <  2:375244/151‑1 (MQ=255)                     cgccgcGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATc                                                                                                                           <  2:567288/149‑1 (MQ=255)                      gccgcGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCAcc                                                                                                                        <  2:846784/151‑1 (MQ=255)                      gccgcGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCAcc                                                                                                                        >  2:605028/1‑151 (MQ=255)                      gccgcGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCAcc                                                                                                                        >  1:406263/1‑151 (MQ=255)                       ccgcGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCa                                                                                                                       <  1:571290/151‑1 (MQ=255)                           gTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCAt                                                                                                                   >  2:723406/1‑151 (MQ=255)                           gTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCAt                                                                                                                   <  2:24820/151‑1 (MQ=255)                              aggagATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTAc                                                                                                                >  1:135396/1‑151 (MQ=255)                                 agaTCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGaa                                                                                                             <  2:787544/151‑1 (MQ=255)                                 agaTCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGa                                                                                                              >  2:336053/1‑150 (MQ=255)                                              aaTGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCt                                                                                                <  1:143425/151‑1 (MQ=255)                                               aTGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTa                                                                                               <  1:622208/151‑1 (MQ=255)                                                 gCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTaa                                                                                              <  2:782060/150‑1 (MQ=255)                                                  cAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACa                                                                                            <  1:218680/151‑1 (MQ=255)                                                  cAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACa                                                                                            <  1:723363/151‑1 (MQ=255)                                                    aaTCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAgg                                                                                          <  1:427218/151‑1 (MQ=255)                                                    aaTCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAgg                                                                                          <  1:342728/151‑1 (MQ=255)                                                    aaTCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGAAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGACATAGAGCGCACCTAACa                                                                                            >  2:574911/1‑149 (MQ=255)                                                       cAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATTGAGCGCGCCTAACAGGAt                                                                                        <  2:495599/150‑1 (MQ=255)                                                       cAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGAt                                                                                        <  2:477956/150‑1 (MQ=255)                                                       cAGCACCAGACCATAAAAGGGACTGTCTGACATCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGAt                                                                                        <  2:421844/150‑1 (MQ=255)                                                        aGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGc                                                                                      >  2:356662/1‑151 (MQ=255)                                                         gTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcc                                                                                     >  2:230536/1‑151 (MQ=255)                                                           aCCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcccc                                                                                   <  1:64515/151‑1 (MQ=255)                                                            ccAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCa                                                                                  <  2:646834/151‑1 (MQ=255)                                                             cAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcccc                                                                                   >  1:24191/1‑149 (MQ=255)                                                                        aGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTa                                                                       >  2:548416/1‑150 (MQ=255)                                                                         gggACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTaat                                                                     <  1:293064/151‑2 (MQ=255)                                                                            aCTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGt                                                                                >  1:283996/1‑137 (MQ=255)                                                                            aCTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGt                                                                                <  2:284021/137‑1 (MQ=255)                                                                                 cTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGGTTTTACCTAAATAACCGc                                                              >  2:504292/1‑150 (MQ=255)                                                                                  tGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCt                                                             >  1:286594/1‑150 (MQ=255)                                                                                    aCAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAAt                                                          <  2:650136/151‑1 (MQ=255)                                                                                       gCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGAc                                                       <  1:78184/151‑1 (MQ=255)                                                                                         aaCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGc                                                     >  1:783380/1‑151 (MQ=255)                                                                                         aaCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGc                                                     >  1:707317/1‑151 (MQ=255)                                                                                         aaCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGc                                                     <  2:15128/151‑1 (MQ=255)                                                                                           cccTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTc                                                   <  2:461556/151‑1 (MQ=255)                                                                                            ccTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCg                                                  <  1:769129/151‑1 (MQ=255)                                                                                                        ccGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTAc                                      >  2:230087/1‑151 (MQ=255)                                                                                                         cGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTAc                                      >  2:551849/1‑150 (MQ=255)                                                                                                             ggTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCt                                 >  1:635984/1‑151 (MQ=255)                                                                                                              gTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                <  2:172650/151‑1 (MQ=255)                                                                                                              gTATGCAGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                <  2:725568/151‑1 (MQ=255)                                                                                                                    aGGTAGGAAGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAtt                          >  1:509645/1‑151 (MQ=255)                                                                                                                           aaGCACTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGcc                     >  1:317879/1‑149 (MQ=255)                                                                                                                               aCTATCGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTa               <  2:406295/151‑1 (MQ=255)                                                                                                                                    cGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaat          <  2:755830/151‑1 (MQ=255)                                                                                                                                    cGTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaa           <  1:387891/150‑1 (MQ=255)                                                                                                                                     gTTGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaat          >  2:236333/1‑150 (MQ=255)                                                                                                                                       tGGTGATGACGGTGGAATAGACGAACATCCAAATCACCAGCATTATGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaataa        >  1:634670/1‑150 (MQ=255)                                                                                                                                            aTGACGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATc  >  2:187190/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                    AATTTTGAATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCATAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATC  >  NC_000913/3257471‑3257760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑