Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,286,334	T→C	L22P (CTT→CCT)	agaI →	galactosamine‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,286,334	0	T	C	100.0%	195.7 / NA	56	L22P (CTT→CCT)	agaI	galactosamine‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/23);  total (33/23)

GTGAAAAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCTTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATCC  >  NC_000913/3286189‑3286480                                                                                                                                                  |                                                                                                                                                   gTGAAAAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGc                                                                                                                                               <  2:624516/151‑1 (MQ=255)   gAAAAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCaa                                                                                                                                             <  1:441248/151‑1 (MQ=255)    aaaaaaGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCa                                                                                                                                              >  2:539503/1‑149 (MQ=255)             cATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACt                                                                                                                                   >  1:854665/1‑151 (MQ=255)                 cGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATAc                                                                                                                               >  2:552110/1‑151 (MQ=255)                    tGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGc                                                                                                                            >  2:6333/1‑151 (MQ=255)                           aaTCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGt                                                                                                                     <  1:687947/151‑1 (MQ=255)                              cGGCGTGACTTTTGTGCTCTCTATTGTTTTTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGa                                                                                                                   <  1:478990/150‑1 (MQ=255)                              cGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGaa                                                                                                                  <  1:121984/151‑1 (MQ=255)                                gCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACg                                                                                                                >  2:505300/1‑151 (MQ=255)                                  gTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTg                                                                                                              >  2:5638/1‑151 (MQ=255)                                   tGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGc                                                                                                             >  1:368124/1‑151 (MQ=255)                                     aCTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCACACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGcc                                                                                                            >  2:800539/1‑150 (MQ=255)                                          tgtgCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGa                                                                                                       >  1:249388/1‑150 (MQ=255)                                               tctctATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAAtattt                                                                                                 >  2:512334/1‑151 (MQ=255)                                                ctctATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAAtattt                                                                                                 >  1:750346/1‑150 (MQ=255)                                                ctctATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAAtatt                                                                                                  <  1:657834/149‑1 (MQ=255)                                                ctctATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAAtatt                                                                                                  <  2:161754/149‑1 (MQ=255)                                                 tctATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAAtatttat                                                                                               >  2:691861/1‑151 (MQ=255)                                                  ctATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAAtattta                                                                                                <  2:425099/149‑1 (MQ=255)                                                       gtttgttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCg                                                                                         >  2:621984/1‑151 (MQ=255)                                                           gttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGa                                                                                      <  2:34179/150‑1 (MQ=255)                                                             tCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATcc                                                                                   >  1:284005/1‑151 (MQ=255)                                                               cGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGt                                                                                 >  1:653614/1‑151 (MQ=255)                                                                gCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTa                                                                                <  2:378723/151‑1 (MQ=255)                                                                  aTTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGc                                                                              >  1:193355/1‑151 (MQ=255)                                                                  aTTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGc                                                                              >  2:627343/1‑151 (MQ=255)                                                                      ggCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAAc                                                                          >  2:698677/1‑151 (MQ=255)                                                                      ggCATTTTGTAAATGGAACGAGGCACTGCGTCTGGCGGGGCCTCTTTACTTAAGGAATTTCATACTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAGTATTTATTGGCCGTGATCCGTAGCAAAc                                                                          >  1:327355/1‑151 (MQ=255)                                                                             tGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATg                                                                    <  2:566891/150‑1 (MQ=255)                                                                             tGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATg                                                                    <  1:642783/150‑1 (MQ=255)                                                                                  aTGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGa                                                              <  1:653649/151‑1 (MQ=255)                                                                                   tGGAACGAGGTACTGCGTCTGGCGGTGCCTCTATACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTTAAAACTATACGGCGTTAAGTGAACGTCCCAGCGAAGATTTATTGGCCCTGGTCCGGAGCAAAGCGCATGCCGTGAt                                                             >  1:284304/1‑151 (MQ=255)                                                                                    ggAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGAt                                                             <  1:172106/150‑1 (MQ=255)                                                                                         gAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCt                                                       >  2:465404/1‑151 (MQ=255)                                                                                         gAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTAATCCTGGGAAAACCCCTAAGCAGGTTGAAAACTATACGGCGTAAAGGGAACGTGCAAGCGAATATTTATGGGCCGTGATCCGAAGCAAACCGAATGCCGGGATTTGc                                                         >  1:150121/1‑149 (MQ=255)                                                                                          aGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTg                                                      <  2:732379/151‑1 (MQ=255)                                                                                          aGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTg                                                      >  2:429386/1‑151 (MQ=255)                                                                                               cTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGAc                                                 >  2:15610/1‑151 (MQ=255)                                                                                                tGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGAcc                                                >  1:558455/1‑151 (MQ=255)                                                                                                tGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGAcc                                                >  2:386496/1‑151 (MQ=255)                                                                                                     cTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAg                                            >  2:444226/1‑150 (MQ=255)                                                                                                     cTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGc                                           >  2:436392/1‑151 (MQ=255)                                                                                                       ggtggtGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCCGCGAATATTTATTGGCCGGGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGTCCGGAGc                                           <  1:835581/149‑1 (MQ=255)                                                                                                          ggtgCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGc                                      <  2:734873/151‑1 (MQ=255)                                                                                                             gCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCAt                                   <  2:441720/151‑1 (MQ=255)                                                                                                             gCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCAt                                   >  2:565393/1‑151 (MQ=255)                                                                                                              ccTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCAtt                                  >  2:469638/1‑151 (MQ=255)                                                                                                                tctTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTAc                                >  1:837270/1‑151 (MQ=255)                                                                                                                 ctTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACt                               >  2:205627/1‑151 (MQ=255)                                                                                                                   ttACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTGGCCTGGCGACCGGAGCCACGCCATTAc                                <  2:104263/148‑1 (MQ=255)                                                                                                                           ggAAATTCGTCCTGTGCATGCACCTCAGCAAGTTGAAAACTATACGGCTTTAAGGGAGCGTGCCAGCGAATATTCATTGGCCGTGAGCCGTTGCAAACTGAATGCCGAGATTTGCCTATCGCCCGGCGCCGCGCCATAACTGACGtctctt                     <  1:298753/151‑3 (MQ=255)                                                                                                                               tttCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATc                 >  1:446528/1‑151 (MQ=255)                                                                                                                                  cATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTg               <  2:519222/150‑1 (MQ=255)                                                                                                                                  cATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTg               <  2:165532/150‑1 (MQ=255)                                                                                                                                              aCCCCTCAGCAAGTGGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATcc  <  2:505363/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GTGAAAAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCTTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATCC  >  NC_000913/3286189‑3286480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑