Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	398,614	A→G	N248S (AAC→AGC)	yaiW →	microcin Bac7 uptake protein; outer membrane surface‑exposed lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	398,614	0	A	G	100.0%	184.8 / NA	53	N248S (AAC→AGC)	yaiW	microcin Bac7 uptake protein; outer membrane surface‑exposed lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/29);  total (24/29)

CCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAG  >  NC_000913/398468‑398759                                                                                                                                                   |                                                                                                                                                  ccGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGcgcg                                                                                                                                               >  1:445837/1‑151 (MQ=255)  cGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCgg                                                                                                                                              >  2:169315/1‑151 (MQ=255)   gTCAGCAAGTCTTCAGTCCCCGCGGCGGGTTGTGGTTTGGTACTTCCCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGcg                                                                                                                                                 <  2:88947/147‑1 (MQ=255)       ggAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAg                                                                                                                                          >  1:556092/1‑150 (MQ=255)            tCTTCAGTCGCCGCGGCGGGTTGGGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGc                                                                                                                                    >  2:832358/1‑151 (MQ=255)                cAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAg                                                                                                                                 <  2:352145/150‑1 (MQ=255)                cAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAg                                                                                                                                 <  2:681184/150‑1 (MQ=255)                cAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGACAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAgc                                                                                                                                >  1:523769/1‑151 (MQ=255)                    cgcggcGGGGGTTGGTGGTTTGTTTCTTACCATTTATTTAATTATCCCGCCAGTTATAGCGCACCGATATCCCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAgcggcg                                                                                                                            <  1:679679/151‑1 (MQ=255)                       cgcgGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTg                                                                                                                          >  2:85659/1‑150 (MQ=255)                           gcggGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCt                                                                                                                     >  1:851472/1‑151 (MQ=255)                           gcggGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCt                                                                                                                     >  1:43990/1‑151 (MQ=255)                               gTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCa                                                                                                                 <  2:819595/151‑1 (MQ=255)                                          gTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGa                                                                                                      <  2:339718/151‑1 (MQ=255)                                                 ccATTTCCAAAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAAtt                                                                                               <  1:347653/151‑1 (MQ=255)                                                  cATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTc                                                                                              >  2:697912/1‑151 (MQ=255)                                                        cTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACg                                                                                        >  1:476537/1‑151 (MQ=255)                                                          gAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACg                                                                                        <  2:589078/149‑1 (MQ=255)                                                           aaTTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGAc                                                                                      <  1:715147/150‑1 (MQ=255)                                                              tATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGt                                                                                   >  2:637446/1‑150 (MQ=255)                                                                tCCCGCCAGTTATAGCGCACCGATATCCCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCGGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTaa                                                                                 >  2:832826/1‑150 (MQ=255)                                                                 cccGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAg                                                                               <  2:236618/151‑1 (MQ=255)                                                                     ccAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAAcc                                                                           >  2:662144/1‑151 (MQ=255)                                                                     ccAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAAc                                                                            <  1:321489/150‑1 (MQ=255)                                                                          tataGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCggg                                                                       >  1:130339/1‑150 (MQ=255)                                                                          tataGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCggg                                                                       <  2:252581/150‑1 (MQ=255)                                                                           ataGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGaa                                                                     <  1:169292/151‑1 (MQ=255)                                                                               cgcACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAAcc                                                                 <  1:407704/151‑1 (MQ=255)                                                                                  aCCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGaa                                                              <  2:126048/151‑1 (MQ=255)                                                                                   ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGaa                                                              >  1:554912/1‑150 (MQ=255)                                                                                   ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAAc                                                             <  2:194947/151‑1 (MQ=255)                                                                                   ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAAc                                                             <  1:393022/151‑1 (MQ=255)                                                                                      atatACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTgg                                                          >  1:148015/1‑151 (MQ=255)                                                                                            cGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTgg                                                          <  2:645051/145‑1 (MQ=255)                                                                                                      aTTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGc                                          >  2:534222/1‑151 (MQ=255)                                                                                                        attAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCATAGCGCAGCCAGTACGGCCAGCGGCGTGAAGCTGGAACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTg                                        >  1:88033/2‑151 (MQ=255)                                                                                                         ttAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTg                                        <  2:300785/150‑1 (MQ=255)                                                                                                          tAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGc                                       <  2:824736/150‑1 (MQ=255)                                                                                                           aaCGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCa                                      >  2:725604/1‑150 (MQ=255)                                                                                                            aCGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCaa                                     >  2:406006/1‑150 (MQ=255)                                                                                                             cGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCaaaa                                   <  1:230289/151‑1 (MQ=255)                                                                                                                    tggTACGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgggg                             >  1:194453/1‑150 (MQ=255)                                                                                                                        aCGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAg                        <  2:420394/151‑1 (MQ=255)                                                                                                                         cGCCAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGCACGGGGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGc                       >  1:367831/1‑151 (MQ=255)                                                                                                                           ccAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGACCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGa                     <  2:223614/151‑1 (MQ=255)                                                                                                                            cAGCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGAc                    >  2:522655/1‑151 (MQ=255)                                                                                                                              gCCGTAATGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAg                  <  2:655809/151‑1 (MQ=255)                                                                                                                                   aaTGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGaa              >  1:660077/1‑150 (MQ=255)                                                                                                                                    aTGCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAAt            <  2:277698/151‑1 (MQ=255)                                                                                                                                      gCCGCGTTTCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTc          <  2:635835/151‑1 (MQ=255)                                                                                                                                             ttCAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTc    <  1:613595/150‑1 (MQ=255)                                                                                                                                               cAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTcgg  <  1:595245/150‑3 (MQ=255)                                                                                                                                               cAGAGCGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAg  <  1:46296/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAG  >  NC_000913/398468‑398759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑