Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,288,276	A→G	N63D (AAT→GAT)	yraI →	putative periplasmic pilin chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,288,276	0	A	G	100.0%	171.0 / NA	45	N63D (AAT→GAT)	yraI	putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/21);  total (24/21)

GCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATAATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACATTAATGTTC  >  NC_000913/3288133‑3288420                                                                                                                                                |                                                                                                                                                 gCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGa                                                                                                                                           <  2:233321/151‑1 (MQ=255)   ttCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATg                                                                                                                                         >  1:101606/1‑151 (MQ=255)    tCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGAt                                                                                                                                          <  1:575875/149‑1 (MQ=255)    tCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATgg                                                                                                                                        >  2:746796/1‑151 (MQ=255)      tGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGt                                                                                                                                       >  1:508012/1‑150 (MQ=255)            ggCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGc                                                                                                                                 <  1:221774/150‑1 (MQ=255)               cAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTg                                                                                                                             <  1:360860/151‑1 (MQ=255)                aGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGc                                                                                                                            >  2:515566/1‑151 (MQ=255)                aGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGc                                                                                                                            >  2:250618/1‑151 (MQ=255)                        gCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCAttt                                                                                                                     <  2:559851/150‑1 (MQ=255)                          aggaggAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATttatt                                                                                                                  >  2:582998/1‑151 (MQ=255)                                 aTCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAAccc                                                                                                           <  1:817384/151‑1 (MQ=255)                                       ttACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCg                                                                                                      <  1:515529/150‑1 (MQ=255)                                         aCAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCt                                                                                                    >  1:217365/1‑150 (MQ=255)                                           aGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTAtt                                                                                                 <  1:422260/151‑1 (MQ=255)                                            gCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTAttt                                                                                                <  1:746753/151‑1 (MQ=255)                                             cGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTc                                                                                               >  1:746952/1‑151 (MQ=255)                                                 cgcgAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCt                                                                                           >  1:45125/1‑151 (MQ=255)                                                    gAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTg                                                                                         >  1:475218/1‑150 (MQ=255)                                                     aGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGACACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTg                                                                                         >  2:655716/1‑149 (MQ=255)                                                             atgatgCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTg                                                                                   <  2:101629/147‑1 (MQ=255)                                                             atgatgCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCg                                                                                >  1:256141/1‑150 (MQ=255)                                                                     aGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACtcat                                                                       >  1:296192/1‑151 (MQ=255)                                                                     aGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACtcat                                                                       >  1:341268/1‑151 (MQ=255)                                                                     aGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACtcat                                                                       <  1:256976/151‑1 (MQ=255)                                                                             agagGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGa                                                               <  1:367067/151‑1 (MQ=255)                                                                              gagGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGa                                                               >  1:254642/1‑150 (MQ=255)                                                                               agGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAat                                                             <  1:569461/151‑1 (MQ=255)                                                                                ggCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAatt                                                            >  1:831817/1‑151 (MQ=255)                                                                                      gTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTaaa                                                      <  2:4390/151‑1 (MQ=255)                                                                                          ccTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAAc                                                    >  1:453030/1‑149 (MQ=255)                                                                                            tGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCt                                                >  1:242097/1‑151 (MQ=255)                                                                                              tCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTg                                               >  1:755536/1‑150 (MQ=255)                                                                                                gCAAACACAGCCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGAAAAAGCCGTGCCCCATTTATTATAACCGCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGACTTATTAACACAGCTGAt                                             >  1:75795/1‑150 (MQ=255)                                                                                                        aGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAAcctgcct                                    >  2:41177/1‑151 (MQ=255)                                                                                                              aGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAAt                              <  2:214815/151‑1 (MQ=255)                                                                                                                        tATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTc                      <  2:833993/149‑1 (MQ=255)                                                                                                                        tATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCg                     <  1:132572/150‑1 (MQ=255)                                                                                                                        tATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGc                    >  2:120170/1‑151 (MQ=255)                                                                                                                                gCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTAc            <  2:34128/151‑1 (MQ=255)                                                                                                                                 cAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACa           <  2:24854/151‑1 (MQ=255)                                                                                                                                     cATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACATTa        >  1:689452/1‑150 (MQ=255)                                                                                                                                      aTGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACATTAAt      <  1:107622/151‑1 (MQ=255)                                                                                                                                        gggTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACATTAATg     <  1:254942/150‑1 (MQ=255)                                                                                                                                          gTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACATTAATGTTc  >  2:381940/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                 GCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATAATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACATTAATGTTC  >  NC_000913/3288133‑3288420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑