Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,311,736 G→A intergenic (‑52/+97) rpsO ← / ← truB 30S ribosomal subunit protein S15/tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,311,7360GA98.4% 227.4 / ‑4.6 65intergenic (‑52/+97)rpsO/truB30S ribosomal subunit protein S15/tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (33/30);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

AGCAGTGCTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTT  >  NC_000913/3311590‑3311879
                                                                                                                                                  |                                                                                                                                               
agcagTGCTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATcc                                                                                                                                             >  2:479570/1‑151 (MQ=255)
 gcagTGCTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATccc                                                                                                                                            <  1:508292/151‑1 (MQ=255)
   agTGCTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAg                                                                                                                                          <  1:796199/151‑1 (MQ=255)
    gTGCTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAgt                                                                                                                                         <  1:669940/151‑1 (MQ=255)
              aaCTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAATTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACg                                                                                                                               <  1:34883/151‑1 (MQ=255)
                    ggTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCaaa                                                                                                                           <  1:177183/149‑1 (MQ=255)
                         aaCCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTaa                                                                                                                    <  2:370376/151‑1 (MQ=255)
                          aCCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTaaa                                                                                                                   <  2:413985/151‑1 (MQ=255)
                           ccGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAAc                                                                                                                  <  2:779076/151‑1 (MQ=255)
                            cGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACa                                                                                                                 <  2:767980/151‑1 (MQ=255)
                            cGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACa                                                                                                                 <  1:268436/151‑1 (MQ=255)
                                  cGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAc                                                                                                           >  1:74886/1‑151 (MQ=255)
                                          tCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGtt                                                                                                   >  1:446899/1‑151 (MQ=255)
                                           cACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGtt                                                                                                   >  1:291018/1‑150 (MQ=255)
                                           cACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGtt                                                                                                   >  1:31177/1‑150 (MQ=255)
                                              gACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGc                                                                                               >  1:477501/1‑151 (MQ=255)
                                              gACCAAACTCAGAAACGATTTGGGCTGATGCTTCAGAACTTAGAGACATTTTAAAACTTCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTAAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAg                                                                                                >  1:695910/1‑150 (MQ=255)
                                                ccAAACTCAGAAACGCATTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCAAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCgg                                                                                             >  1:665402/1‑151 (MQ=255)
                                                ccAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCgg                                                                                             >  1:191520/1‑151 (MQ=255)
                                                  aaaCTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCa                                                                                           <  2:390867/151‑1 (MQ=255)
                                                  aaaCTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGCACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGc                                                                                            >  1:20388/1‑150 (MQ=255)
                                                       cAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTAt                                                                                       <  1:839903/150‑1 (MQ=255)
                                                          aaaCGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCt                                                                                    >  2:804462/1‑150 (MQ=255)
                                                            aCGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACt                                                                                 >  1:476269/1‑151 (MQ=255)
                                                            aCGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACt                                                                                 <  2:789498/151‑1 (MQ=255)
                                                             cGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTc                                                                                >  2:695969/1‑151 (MQ=255)
                                                                  ttAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGc                                                                           >  1:543425/1‑151 (MQ=255)
                                                                  ttAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGc                                                                           >  1:54527/1‑151 (MQ=255)
                                                                    aGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCg                                                                          <  1:269987/150‑1 (MQ=255)
                                                                    aGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCg                                                                          <  2:752099/150‑1 (MQ=255)
                                                                    aGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGa                                                                         <  1:130269/151‑1 (MQ=255)
                                                                     gCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGAAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGAc                                                                        >  1:526092/1‑151 (MQ=255)
                                                                      cTGTTGCTTCAGTACTTAGAGACATTTTAAACCTCCAAAGTATATAGAATGAAAGGACGCCAATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGGTAAACAATTTACGCTACGTTAAGCGGCAGTATTCTACTCGTAGCGAc                                                                        <  1:255122/150‑1 (MQ=255)
                                                                        gTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGAcc                                                                       <  1:625675/149‑1 (MQ=255)
                                                                        gTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTg                                                                     >  2:528312/1‑151 (MQ=255)
                                                                        gTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTg                                                                     <  1:256424/151‑1 (MQ=255)
                                                                        gTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTg                                                                     <  1:445077/151‑1 (MQ=255)
                                                                           gCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTa                                                                  <  1:609074/151‑1 (MQ=255)
                                                                           gCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTa                                                                  <  1:326727/151‑1 (MQ=255)
                                                                            cTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTAt                                                                 >  1:332859/1‑151 (MQ=255)
                                                                              tCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTAt                                                                 <  2:4409/149‑1 (MQ=255)
                                                                                    cTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGAc                                                         >  1:129946/1‑151 (MQ=255)
                                                                                     ttAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACg                                                        >  1:231784/1‑151 (MQ=255)
                                                                                      tAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACgg                                                       >  1:727197/1‑151 (MQ=255)
                                                                                          gccATTTTAAAACTCCAAAGTATATAGAATGAAACGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTa                                                    >  1:765712/3‑150 (MQ=255)
                                                                                             aTTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACAt                                                <  1:506522/151‑1 (MQ=255)
                                                                                               tttAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACAtt                                               >  2:151463/1‑150 (MQ=255)
                                                                                                 tAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACg                                            >  1:253810/1‑151 (MQ=255)
                                                                                                  aaaaCTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACg                                            <  2:291043/150‑1 (MQ=255)
                                                                                                           aaGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTc                                  >  1:462812/1‑151 (MQ=255)
                                                                                                            aGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTAtt                                   >  1:642312/1‑149 (MQ=255)
                                                                                                             gTATATAGAATGAAAGGAAGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACATGTTATCGCAAGACGGTTAACATTACGCCGGGTAt                                    >  2:686344/1‑147 (MQ=255)
                                                                                                               atataGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAacc                              >  2:471627/1‑151 (MQ=255)
                                                                                                               atataGAATGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAacc                              >  2:328080/1‑151 (MQ=255)
                                                                                                                     aaTGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAgg                        <  1:280187/151‑1 (MQ=255)
                                                                                                                     aaTGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAgg                        <  1:577350/151‑1 (MQ=255)
                                                                                                                      aTGAAAGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGc                       >  2:662780/1‑151 (MQ=255)
                                                                                                                          aaGGACGCCGATCTCTAATTCAGCTATCCCAGAGTACGTTACGCAAAGGGGTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGcgac                    >  1:51010/1‑150 (MQ=255)
                                                                                                                          aaGGACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGcgacg                   >  1:184238/1‑151 (MQ=255)
                                                                                                                             gACGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAgg                >  1:133111/1‑151 (MQ=255)
                                                                                                                              aCGCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGgc               <  1:482107/151‑1 (MQ=255)
                                                                                                                                gCCGATCTCTAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGgcgc             >  2:62387/1‑151 (MQ=255)
                                                                                                                                        ctAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAAcgcg       <  1:563312/149‑1 (MQ=255)
                                                                                                                                        ctAATTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGc     >  2:372039/1‑151 (MQ=255)
                                                                                                                                           aTTCAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCtt  <  2:124381/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AGCAGTGCTACCTGAACTTCGGTAGAACCGGTGTCGTTTGCGTCACGACCAAACTCAGAAACGATTTTAGCTGTTGCTTCAGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTT  >  NC_000913/3311590‑3311879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: