Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,434,764	T→C	L184S (TTG→TCG)	fmt →	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,434,764	0	T	C	100.0%	106.4 / NA	31	L184S (TTG→TCG)	fmt	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/17);  total (14/17)

GTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTTGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCG  >  NC_000913/3434622‑3434901                                                                                                                                                |                                                                                                                                           gtgACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAaacaac                                                                                                                                    >  2:207880/1‑151 (MQ=255) gtgACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAaacaa                                                                                                                                     <  2:825097/150‑1 (MQ=255)  tgACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACt                                                                                                                                   >  2:388376/1‑151 (MQ=255)    aCCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCGGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTgg                                                                                                                                 <  2:230667/151‑1 (MQ=255)    aCCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTgg                                                                                                                                 >  1:699710/1‑151 (MQ=255)             cAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGacggc                                                                                                                         >  1:403810/1‑150 (MQ=255)              aaaTGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGacggcac                                                                                                                       >  2:639678/1‑151 (MQ=255)                aTGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGacggcacgg                                                                                                                     <  1:10092/151‑1 (MQ=255)                        cGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAAcc                                                                                                              >  2:19342/1‑150 (MQ=255)                        cGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCa                                                                                                             <  1:809150/151‑1 (MQ=255)                            ttAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGa                                                                                                           >  2:676012/1‑149 (MQ=255)                                       tGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGa                                                                                               >  2:148795/1‑150 (MQ=255)                                        gATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGATGaa                                                                                              <  1:512002/150‑1 (MQ=255)                                              ctctATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTg                                                                                      >  1:713569/1‑151 (MQ=255)                                                           ccTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATTACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCga                                                                         <  2:728785/151‑1 (MQ=255)                                                           ccTGCCCGATT‑CTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGcc                                                                           <  2:86138/148‑1 (MQ=255)                                                                   aTTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTg                                                                  >  1:237184/1‑150 (MQ=255)                                                                         gCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAATGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCTCTTACGCCGAGAAGTTGAGTaa                                                             <  1:301567/149‑1 (MQ=255)                                                                               gaTACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAaagaagaag                                                     <  1:88153/151‑1 (MQ=255)                                                                                aTACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAgc                                                    <  1:812468/151‑1 (MQ=255)                                                                                aTACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAgc                                                    <  2:787547/151‑1 (MQ=255)                                                                                        ggTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTAtt                                             <  1:416960/150‑1 (MQ=255)                                                                                          tACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGAc                                          >  1:661673/1‑151 (MQ=255)                                                                                            cGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTg                                        <  2:287946/151‑1 (MQ=255)                                                                                                  aCGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACt                                  >  1:3004/1‑151 (MQ=255)                                                                                                     aCAAGCTGACAGAGCCTGTCCCACAAGGGCTTATCTCC‑CCGTCGAAACAACTGGCAGACGGCACGGCGATACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGATGTTGGGTAAAGAATAGGGGCGTATTGACTGGTCTCttt                                <  1:613126/150‑1 (MQ=255)                                                                                                       aggCTGGAAGAGCTTGGCCCACAA‑GGCTTAACCCCAAGGCCAAAACAATGGGCAGCCGGACCGGCG‑AACCAAAAAGTCAAGACCAAACCCCTTGTC‑CTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCg                              <  2:203696/147‑1 (MQ=255)                                                                                                       aaGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCgg                             >  1:24859/1‑151 (MQ=255)                                                                                                                     tGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGa                <  2:798691/150‑1 (MQ=255)                                                                                                                      ggCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAAc              <  1:639636/151‑1 (MQ=255)                                                                                                                                  cTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTcgcg  >  2:548290/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                           GTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTTGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCG  >  NC_000913/3434622‑3434901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑