Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	411,745	A→G	L246P (CTA→CCA)	araJ ←	L‑arabinose‑inducible putative transporter, MFS family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	411,745	0	A	G	100.0%	191.4 / NA	52	L246P (CTA→CCA)	araJ	L‑arabinose‑inducible putative transporter, MFS family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/30);  total (22/30)

AATAAGCGACGTTGTTTTCATGCCGCCGCAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTG  >  NC_000913/411600‑411891                                                                                                                                                  |                                                                                                                                                   aaTAAGCGACGTTGTTTTCATGCCGCCGCAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGccc                                                                                                                                                >  1:222793/1‑150 (MQ=255) aaTAAGCGACGTTGTTTTCATGCCGCCGCAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCa                                                                                                                                               <  1:586392/151‑1 (MQ=255)    aaGCGACGTTGTTTTCATGCCGCCGCAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACt                                                                                                                                            <  2:519773/151‑1 (MQ=255)    aaGCGACGTTGTTTTCATGCCGCCGCAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAAGCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAAc                                                                                                                                             <  1:788481/150‑1 (MQ=255)       cGACGTTGTTTTCATGCCGCCGCAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAAc                                                                                                                                         >  1:291507/1‑151 (MQ=255)         aCGTTGTTTTCATGCCGCCGCAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAAcat                                                                                                                                       >  1:684561/1‑151 (MQ=255)             tgttTTCATGCCGCCGCAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAAcatcat                                                                                                                                    >  2:384405/1‑150 (MQ=255)                        cgccgcAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCAt                                                                                                                        >  1:143189/1‑151 (MQ=255)                         gccgcAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATc                                                                                                                       >  2:261484/1‑151 (MQ=255)                          ccgcAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCg                                                                                                                      <  1:832181/151‑1 (MQ=255)                           cgcAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGc                                                                                                                     >  2:673299/1‑151 (MQ=255)                                aaaaaGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGttt                                                                                                                <  1:259781/151‑1 (MQ=255)                                aaaaaGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGttt                                                                                                                >  2:371934/1‑151 (MQ=255)                                  aaaGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCGCAACTAACATCATAATAAAGGTCATCGCCGTTTcc                                                                                                              <  2:733674/151‑1 (MQ=255)                                      agCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGaaa                                                                                                          <  1:130486/151‑1 (MQ=255)                                            agcagTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCg                                                                                                     >  2:358291/1‑150 (MQ=255)                                             gcagTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCgg                                                                                                    <  1:279097/150‑1 (MQ=255)                                                gTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAAt                                                                                                <  2:291532/151‑1 (MQ=255)                                                  gCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATa                                                                                               <  1:338018/150‑1 (MQ=255)                                                    cAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAAc                                                                                            <  1:202175/151‑1 (MQ=255)                                                         cAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAAcatcat                                                                                       >  2:642874/1‑151 (MQ=255)                                                             tataAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTAt                                                                                   >  2:219085/1‑151 (MQ=255)                                                              ataAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATg                                                                                  <  2:654278/151‑1 (MQ=255)                                                               taAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATgg                                                                                 >  2:854549/1‑151 (MQ=255)                                                                  agtcagtcACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCtt                                                                              >  1:746236/1‑151 (MQ=255)                                                                        tcaCTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGt                                                                         >  1:294699/1‑150 (MQ=255)                                                                        tcaCTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGt                                                                         >  1:801652/1‑150 (MQ=255)                                                                         caCTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAg                                                                       <  2:255826/151‑1 (MQ=255)                                                                         caCTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAAT‑AAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGc                                                                      <  1:784601/151‑1 (MQ=255)                                                                             gctgcAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGa                                                                   <  1:457133/151‑1 (MQ=255)                                                                               tgcAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAAc                                                                 >  1:310277/1‑151 (MQ=255)                                                                                gcAATGCTCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACAGCATGTATGGCTTTACGTAGTTGaa                                                                  <  1:515109/149‑1 (MQ=255)                                                                                gcAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGaa                                                                  <  1:209078/149‑1 (MQ=255)                                                                                 cAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCa                                                               <  1:114940/151‑1 (MQ=255)                                                                                               gAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCt                                                  <  1:89519/150‑1 (MQ=255)                                                                                               gAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTg                                                 >  2:188611/1‑151 (MQ=255)                                                                                                    aCGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTg                                            <  1:801492/151‑1 (MQ=255)                                                                                                      gTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGcc                                          <  1:316507/151‑1 (MQ=255)                                                                                                          tGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAAc                                      >  2:501811/1‑151 (MQ=255)                                                                                                           gAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACa                                     <  1:116048/151‑1 (MQ=255)                                                                                                           gAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTAGCCaaa                                       <  1:854490/149‑1 (MQ=255)                                                                                                             aaTCCTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATc                                   <  1:174098/151‑1 (MQ=255)                                                                                                              aTCCTGCCACTTAGAATATTTCCAAGCACCATCCCTGTCCCAACTAACATCATAATAAAGGTCTTCGCCTTTTCCGAAAAACCGAAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACaac                                   <  1:735687/150‑3 (MQ=255)                                                                                                                 cTGCCGCTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAGAGGGCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGCAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTgg                               >  1:108886/1‑151 (MQ=255)                                                                                                                 cTGCCACTTAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTgg                               >  1:677424/1‑151 (MQ=255)                                                                                                                         tAGCATATTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGaa                        >  1:135475/1‑150 (MQ=255)                                                                                                                               atTTCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAAc                 <  2:303464/151‑1 (MQ=255)                                                                                                                                tttCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAAcc                >  2:103930/1‑151 (MQ=255)                                                                                                                                 ttCCCAGCACCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAAcc                <  1:674005/150‑1 (MQ=255)                                                                                                                                         aCCATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCg       <  2:66185/151‑1 (MQ=255)                                                                                                                                          ccATCCCTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCg       <  2:179603/150‑1 (MQ=255)                                                                                                                                              cccTGGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTg  <  1:395321/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   AATAAGCGACGTTGTTTTCATGCCGCCGCAGAAAAAGAGCATCAGCAGTGCCAGTACAATTATAAAGTCAGTCACTGCTGCAATGCGCAGTGGTGAATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTG  >  NC_000913/411600‑411891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑