Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,636,796	C→A	Q205H (CAG→CAT)	yhiN ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,636,796	0	C	A	100.0%	133.8 / NA	37	Q205H (CAG→CAT)	yhiN	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/21);  total (16/21)

AATTCCCCCGGTTGCC‑AGTAGCTTGAAATCTGCAACACCGCCGG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGC  >  NC_000913/3636656‑3636939                                                                                                                                                |                                                                                                                                                aaTTCCCCCGGTTTCCAAGTGGTTAAAATTCTGAACCCCCGCCGG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCCCGAAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTa                                                                                                                                             <  2:344854/146‑1 (MQ=255)       cccGGGTGCC‑AGTTATTTAAAATTCGCCAACCCCCCGGTTCCAAACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGAAAAACGGGGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGc                                                                                                                                  <  2:765419/151‑1 (MQ=255)          ggTTGCC‑AGTAGCTTGAAATCTGCAACACCGCCGG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACg                                                                                                                              >  2:347691/1‑151 (MQ=255)                                        cgccgG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGAccc                                                                                                 >  1:743141/1‑151 (MQ=255)                                           cgG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcg                                                                                              >  2:827241/1‑151 (MQ=255)                                             g‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgcg                                                                                            >  1:348511/1‑151 (MQ=255)                                                    aCAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCgg                                                                                      >  2:295587/1‑151 (MQ=255)                                                    aCAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGAAAACCGGTGCCGTTTTCAGCGGT‑AACCACGAAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTc                                                                                        <  2:439702/149‑1 (MQ=255)                                                     cAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGgc                                                                                     >  1:514123/1‑151 (MQ=255)                                                       acccccgGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCCGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGt                                                                                         >  1:425007/6‑145 (MQ=255)                                                          cgcgGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCAc                                                                                >  1:531727/1‑151 (MQ=255)                                                             ggtgggtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGtt                                                                             >  2:534468/1‑151 (MQ=255)                                                             ggtgggtgAAGAGAAAGTTCCCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACg                                                                               <  2:301190/149‑1 (MQ=255)                                                             ggtgggtaAAGAGTAAGTTCCCAGGAAACCCGCCGCTGTTTACAGCGGC‑ATCCCCGGAAGACCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGtt                                                                             <  2:649306/151‑1 (MQ=255)                                                                     aaGAGTAAGTTCTCACGGAAACGGGTGCCGTTTTCAGCGTT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGcc                                                                       <  1:213221/149‑1 (MQ=255)                                                                     aaGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCa                                                                      >  2:738814/1‑150 (MQ=255)                                                                     aaGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCa                                                                      >  1:7086/1‑150 (MQ=255)                                                                     aaGAGTAAGTTCCCACGGAAACCGGTGCCGTTTTCAGCGTT‑AACCACGAAAGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCaa                                                                     <  2:648990/151‑1 (MQ=255)                                                                      agagTAAGTTCGCCCGAAAACGGGTCCCGTTTACACCGGT‑AGCCACGGAAGTCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCaa                                                                     <  2:202016/150‑1 (MQ=255)                                                                           agtttccccgggAAAACGGTGCCGTTTTCAGCGTT‑AATCACGGAAGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGt                                                                <  1:54697/140‑1 (MQ=255)                                                                           aaGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGAAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGtt                                                               <  2:601489/151‑1 (MQ=255)                                                                           aaGTTCTCACGAAAACCGGTCCCTTTTTCGGCGGT‑AATCCGGGAGGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGtt                                                               <  2:319352/151‑1 (MQ=255)                                                                            attcctgaCGAAATACTGTCCCTTTTTCTTCTGT‑AATCGGGGACGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCCACGCTTCACGCAGAGTCAATGCCCCCACACCCCCCCGCGTCCGCAGCCCGTTGAGGCCAAAtt                                                                  <  1:200773/140‑1 (MQ=255)                                                                                 tcACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCa                                                          >  2:153951/1‑150 (MQ=255)                                                                                  cACGGAAAAGGGTGCCGTTTCCAGCGGT‑AACCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAAt                                                        <  2:531764/151‑1 (MQ=255)                                                                                       aaaaGGGTGCC‑TTTTCCGGGGTAAATCACGAAAGCCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATc                                                       <  1:336543/147‑1 (MQ=255)                                                                                       aaaaCGGTCCCTTTTTAGGGGGT‑AATCCCGAAGGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTtat                                                   <  1:558855/151‑1 (MQ=255)                                                                                            ggTGCCTTTTTCAGCGTT‑AATCCCGAAGGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAAc                                                <  2:602419/149‑1 (MQ=255)                                                                                            ggTCCCTTTTCCGGCGGA‑AACCACGGAAGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAAcc                                               <  1:23449/150‑1 (MQ=255)                                                                                                ccGTTTCCAGGGTA‑AACCACGAAGGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCaaa                                            <  1:625115/149‑1 (MQ=255)                                                                                                 cGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACTCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGAGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACg                                          >  1:94167/1‑150 (MQ=255)                                                                                                  gTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGc                                        >  1:283833/1‑151 (MQ=255)                                                                                                           gtt‑AATCCGGGAAGGCACCGCCCCGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGa                                      <  2:803998/142‑1 (MQ=255)                                                                                                                     ggAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGc                      >  2:663222/1‑151 (MQ=255)                                                                                                                         gcccccGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTg                  <  1:534431/147‑1 (MQ=255)                                                                                                                                       cgccAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTc    <  2:438311/151‑1 (MQ=255)                                                                                                                                         ccAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTcgc  >  1:224223/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                AATTCCCCCGGTTGCC‑AGTAGCTTGAAATCTGCAACACCGCCGG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGC  >  NC_000913/3636656‑3636939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑