Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,687,764	T→C	A470A (GCA→GCG)	bcsC ←	cellulose synthase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,687,764	0	T	C	100.0%	124.6 / NA	36	A470A (GCA→GCG)	bcsC	cellulose synthase subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/23);  total (13/23)

CGCATTAACGTATCGGCCTGGCTGCGTTGTCCGGCCTGCCAGAGATCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCA  >  NC_000913/3687618‑3687909                                                                                                                                                   |                                                                                                                                                  cgcATTAACGTATCGGCCTGGCTGCGTTGTCCGGCCTGCCAGAGATCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCct                                                                                                                                               <  1:656490/151‑1 (MQ=255)          gTATCGGCCTGGCTGCGTTGTCCGGCCTGCCAGAGATCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCctgct                                                                                                                                      >  2:749752/1‑151 (MQ=255)                    ggCTGCGTTGTCCGGCCTGCCAGAGATCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGAc                                                                                                                             <  1:794515/150‑1 (MQ=255)                          gTTGTCCGGCCTGCCAGAGATCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGtt                                                                                                                      <  1:459924/151‑1 (MQ=255)                                cGGCCTGCCAGAGATCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAg                                                                                                                <  2:91557/151‑1 (MQ=255)                                       ccAGAGATCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCg                                                                                                          >  1:385570/1‑150 (MQ=255)                                       ccAGAGATCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGt                                                                                                         >  1:474439/1‑151 (MQ=255)                                        cAGAGATCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGtt                                                                                                        <  1:133341/151‑1 (MQ=255)                                            gaTCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGat                                                                                                    <  1:356991/151‑1 (MQ=255)                                                cTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGatat                                                                                                  <  1:492054/149‑1 (MQ=255)                                                 tGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATatcat                                                                                               >  1:543379/1‑151 (MQ=255)                                                   cGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATatcatc                                                                                              >  1:772207/1‑150 (MQ=255)                                                        gTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCCGTCGTTGCCGCTGAAGTGCTGCCTCCTGCGCCCATTTGCCCTGGTTTTCCTGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCt                                                                                        <  1:542569/151‑1 (MQ=255)                                                        gTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCt                                                                                        <  1:508093/151‑1 (MQ=255)                                                            gtaagtaaTCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTAcgc                                                                                    <  2:675825/151‑1 (MQ=255)                                                               agtaaTCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTAcgccgc                                                                                 >  2:21244/1‑151 (MQ=255)                                                                gtaaTCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCt                                                                                <  2:374847/151‑1 (MQ=255)                                                                     ccATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTg                                                                           <  2:702854/151‑1 (MQ=255)                                                                      cATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTgg                                                                          <  1:441490/151‑1 (MQ=255)                                                                         aCGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGc                                                                         >  1:607336/1‑149 (MQ=255)                                                                              gCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCagagag                                                                   >  1:712537/1‑150 (MQ=255)                                                                                       cAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATa                                                         <  2:445255/151‑1 (MQ=255)                                                                                        aGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATa                                                         >  2:491971/1‑150 (MQ=255)                                                                                               gTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTc                                                 >  2:613637/1‑151 (MQ=255)                                                                                                 cGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCa                                                <  2:369262/150‑1 (MQ=255)                                                                                                    tGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCtt                                            >  2:571969/1‑151 (MQ=255)                                                                                                           gAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCt                                       <  1:652421/149‑1 (MQ=255)                                                                                                              gTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGa                                  <  2:631497/151‑1 (MQ=255)                                                                                                                gctgcCGCCTGCGCCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGAtt                                <  2:772263/151‑1 (MQ=255)                                                                                                                            gcCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTaa                     >  2:154661/1‑150 (MQ=255)                                                                                                                            gcCCATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTa                      >  2:716037/1‑149 (MQ=255)                                                                                                                             cccATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAat                   <  1:470505/151‑1 (MQ=255)                                                                                                                               cATTTGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAatat                 <  1:491935/151‑1 (MQ=255)                                                                                                                                  ttGCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATAttt               <  2:303664/150‑1 (MQ=255)                                                                                                                                    gCCCTGGTTTTCCAGCGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGc             <  1:154638/150‑1 (MQ=255)                                                                                                                                                cAGCGCCTGTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCATCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCa  <  1:224414/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CGCATTAACGTATCGGCCTGGCTGCGTTGTCCGGCCTGCCAGAGATCCTGCGAAAGTCGGTAAGTAATCCATACGCTGCCGGGGTCCAGCGCCAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCA  >  NC_000913/3687618‑3687909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑