Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,930,513	T→C	Q194R (CAG→CGG)	ravA ←	hexameric AAA+ MoxR family ATPase, putative molecular chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,930,513	0	T	C	100.0%	163.1 / NA	46	Q194R (CAG→CGG)	ravA	hexameric AAA+ MoxR family ATPase, putative molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/30);  total (16/30)

GTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCTGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATCGGG  >  NC_000913/3930367‑3930659                                                                                                                                                   |                                                                                                                                                   gTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTg                                                                                                                                                <  2:801984/151‑1 (MQ=255)        aTCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTc                                                                                                                                         <  1:131375/151‑1 (MQ=255)               tGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATg                                                                                                                                   <  2:171332/150‑1 (MQ=255)                 cTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATgg                                                                                                                                  >  1:300951/1‑149 (MQ=255)                     cgcAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGa                                                                                                                            <  2:364559/151‑1 (MQ=255)                          caTAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTc                                                                                                                       <  1:127771/151‑1 (MQ=255)                              aaaaTCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCtt                                                                                                                   >  1:421963/1‑151 (MQ=255)                                   cAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATcc                                                                                                              <  2:424652/151‑1 (MQ=255)                                   cAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATcc                                                                                                              <  2:341729/151‑1 (MQ=255)                                       tCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGc                                                                                                           >  1:606527/1‑150 (MQ=255)                                        cAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGc                                                                                                           <  1:835301/149‑1 (MQ=255)                                        cAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCa                                                                                                          <  2:562260/150‑1 (MQ=255)                                        cAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCAACCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCa                                                                                                          <  2:229152/150‑1 (MQ=255)                                         aaaTACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACt                                                                                                        >  2:568445/1‑151 (MQ=255)                                          aaTACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACtt                                                                                                       >  1:799626/1‑151 (MQ=255)                                                 gATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTaa                                                                                                <  2:69777/151‑1 (MQ=255)                                                  aTCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAAc                                                                                               <  1:24512/151‑1 (MQ=255)                                                  aTCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAAc                                                                                               >  2:36292/1‑151 (MQ=255)                                                    cGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACca                                                                                             <  2:744045/151‑1 (MQ=255)                                                     gggCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACcac                                                                                            <  1:623758/151‑1 (MQ=255)                                                          gCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACg                                                                                       >  2:77664/1‑151 (MQ=255)                                                             tAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCCTCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGa                                                                                      >  1:607752/1‑149 (MQ=255)                                                               aTTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATc                                                                                   >  1:594693/1‑150 (MQ=255)                                                               aTTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCa                                                                                  <  2:267443/151‑1 (MQ=255)                                                                     ccAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGc                                                                            <  2:300976/151‑1 (MQ=255)                                                                        aTCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCgg                                                                          <  1:812137/150‑1 (MQ=255)                                                                            ctTTCTGCCTGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGTGCGGGAATTCGCTTTATCCTGCGCTTTATCTAACCACAGACGAATCAGCATGCGGt                                                                         <  1:538995/147‑1 (MQ=255)                                                                            ctTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGt                                                                         <  1:594706/147‑1 (MQ=255)                                                                                   ccAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCt                                                              >  1:77602/1‑151 (MQ=255)                                                                                   ccAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCt                                                              >  2:561156/1‑151 (MQ=255)                                                                                   ccAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCt                                                              <  2:44483/151‑1 (MQ=255)                                                                                           cATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGAct                                                      <  2:793720/151‑1 (MQ=255)                                                                                           cATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGAct                                                      <  1:293971/151‑1 (MQ=255)                                                                                               ttcttcATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGActgctg                                                  >  2:386832/1‑151 (MQ=255)                                                                                                        tgtgACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTcc                                         >  2:541797/1‑151 (MQ=255)                                                                                                        tgtgACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTcc                                         >  1:726624/1‑151 (MQ=255)                                                                                                         gtgACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTcc                                         <  2:802207/150‑1 (MQ=255)                                                                                                           gACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGc                                      >  2:421505/1‑151 (MQ=255)                                                                                                            aCCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCa                                     <  1:185869/151‑1 (MQ=255)                                                                                                             ccTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAg                                    <  1:622089/151‑1 (MQ=255)                                                                                                             ccTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAg                                    <  1:547166/151‑1 (MQ=255)                                                                                                                                          ttttCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGgccg                      >  1:804844/1‑136 (MQ=255)                                                                                                                                          ttttCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGgccg                      <  2:804900/136‑1 (MQ=255)                                                                                                                                            ttCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCAt      <  1:68413/150‑1 (MQ=255)                                                                                                                                              cATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATCgg   <  1:578660/151‑1 (MQ=255)                                                                                                                                               aTCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATCggg  <  2:591281/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCTGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATCGGG  >  NC_000913/3930367‑3930659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑