Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,969,685	A→G	I219V (ATC→GTC)	wzzE →	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,969,685	0	A	G	100.0%	219.3 / NA	61	I219V (ATC→GTC)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/32);  total (29/32)

CTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCA  >  NC_000913/3969542‑3969824                                                                                                                                                |                                                                                                                                            cTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACg                                                                                                                                       >  2:71115/1‑150 (MQ=255)   aataaCCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGa                                                                                                                                      <  1:821197/149‑1 (MQ=255)    ataaCCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGAccg                                                                                                                                   <  2:669790/151‑1 (MQ=255)    ataaCCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGAccg                                                                                                                                   <  1:280573/151‑1 (MQ=255)     taaCCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGAccgc                                                                                                                                  >  2:785467/1‑151 (MQ=255)      aaCCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGAccgcc                                                                                                                                 >  1:302263/1‑151 (MQ=255)        ccTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCgg                                                                                                                               >  1:775579/1‑151 (MQ=255)           gTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATg                                                                                                                            <  2:82224/151‑1 (MQ=255)              aCGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAAc                                                                                                                         >  1:180973/1‑151 (MQ=255)                gTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAg                                                                                                                       <  1:592017/151‑1 (MQ=255)                   aGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCAt                                                                                                                    >  2:796708/1‑151 (MQ=255)                    gTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCAtt                                                                                                                   <  2:327030/151‑1 (MQ=255)                      aTGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTg                                                                                                                  >  1:699456/1‑150 (MQ=255)                       tGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGa                                                                                                                 <  1:173519/150‑1 (MQ=255)                        gTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAg                                                                                                                <  1:107740/150‑1 (MQ=255)                        gTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGc                                                                                                               <  2:505707/151‑1 (MQ=255)                          tGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGAccgcc                                                                                                                                 >  1:404872/1‑131 (MQ=255)                          tGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCa                                                                                                              <  1:394308/150‑1 (MQ=255)                          tGCTTTTGCCAGCCAGCGTGCAGCCACCAATCGGAATGATGAGCTGAAGGGCGCATGGCCGGCCGGTCCCATCAAGAGGAAGGTTCGGGTGAAGGGTCGGGAAGAGGTGGCGAAGGCCGCCTACGCCCGCGGGATGAAcggatt                                                                                                                    >  1:435449/1‑139 (MQ=255)                           gCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGGGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGAccgcc                                                                                                                                 <  2:404904/130‑1 (MQ=255)                           gCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAgg                                                                                                            >  2:707178/1‑151 (MQ=255)                           gCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAg                                                                                                             <  1:796652/150‑1 (MQ=255)                                tGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCt                                                                                                        <  2:121585/150‑1 (MQ=255)                                tGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTg                                                                                                       >  1:810396/1‑151 (MQ=255)                                  ccagccagCGTGCAGCCAGCCATCTGAATGGTGAGCTGAAAGGCGCATGGGCGGCGCGTGCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGaa                                                                                                     <  1:814795/151‑1 (MQ=255)                                    agccagcGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGaaa                                                                                                    >  2:616824/1‑150 (MQ=255)                                    agccagcGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGaaa                                                                                                    >  1:91679/1‑150 (MQ=255)                                       cagcGTGCAGCCAGACATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGAGGAAAGATCAGGGGAAGCGGCAGGAGGAGGCGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTg                                                                                                >  1:140612/1‑151 (MQ=255)                                         gcGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCt                                                                                              <  1:454402/151‑1 (MQ=255)                                          cGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTg                                                                                             >  2:753821/1‑151 (MQ=255)                                            tGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTg                                                                                             >  1:529287/1‑149 (MQ=255)                                                    gccaTCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCata                                                                                    >  2:158484/1‑150 (MQ=255)                                                      caTCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataata                                                                                 <  1:448866/151‑1 (MQ=255)                                                           gAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAtt                                                                               <  2:477783/148‑1 (MQ=255)                                                           gAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTcg                                                                            <  1:749006/151‑1 (MQ=255)                                                             atgatgAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTcgcg                                                                          >  1:236246/1‑151 (MQ=255)                                                               gatgaGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCgcgc                                                                         <  1:71092/150‑1 (MQ=255)                                                               gatgaGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTCCgcgc                                                                         <  2:106638/150‑1 (MQ=255)                                                                    gCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCg                                                                   >  2:214351/1‑151 (MQ=255)                                                                    gCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCg                                                                   <  2:371262/151‑1 (MQ=255)                                                                     cTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCg                                                                   >  2:699398/1‑150 (MQ=255)                                                                        aaaGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGAc                                                                 >  2:60521/1‑149 (MQ=255)                                                                            gcgcATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGt                                                           <  2:29871/151‑1 (MQ=255)                                                                               cATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAcc                                                        >  1:397840/1‑151 (MQ=255)                                                                                aTGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCt                                                       <  1:727516/151‑1 (MQ=255)                                                                                aTGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCt                                                       <  2:654137/151‑1 (MQ=255)                                                                                aTGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCt                                                       <  2:753136/151‑1 (MQ=255)                                                                                         gcgTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGa                                               >  2:557552/1‑150 (MQ=255)                                                                                           gTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAAtt                                            <  1:158461/151‑1 (MQ=255)                                                                                             aCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTa                                           <  1:112150/150‑1 (MQ=255)                                                                                             aCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTa                                           <  1:609625/150‑1 (MQ=255)                                                                                             aCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTAc                                          >  1:584774/1‑151 (MQ=255)                                                                                              ccATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTAAAAATTGCTGAGCAGCATAATTTTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTa                                           <  1:554626/149‑1 (MQ=255)                                                                                                      aTGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTc                                   >  2:641164/1‑149 (MQ=255)                                                                                                      aTGAAAGCCCAGGGGAAGCGACCGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGTTGAACAGCATTGAGCATGCGCTGTAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTc                                   >  1:439503/1‑149 (MQ=255)                                                                                                          aaGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAAt                             <  1:477960/151‑1 (MQ=255)                                                                                                                aGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCt                       <  2:268502/151‑1 (MQ=255)                                                                                                                        cGTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGc               >  2:414888/1‑151 (MQ=255)                                                                                                                         gTCAGGAAGAGGTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCg              >  1:226156/1‑151 (MQ=255)                                                                                                                                    gTGGCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTc   >  1:466826/1‑151 (MQ=255)                                                                                                                                      ggCGAAAGCCGTCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCa  <  1:214327/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                            CTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTGCAGCCAGCCATCTGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCA  >  NC_000913/3969542‑3969824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑