Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	443,420	A→G	G74G (GGT→GGC)	yajL ←	oxidative‑stress‑resistance chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,420	0	A	G	100.0%	116.8 / NA	35	G74G (GGT→GGC)	yajL	oxidative‑stress‑resistance chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/16);  total (19/16)

ATTACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACC  >  NC_000913/443273‑443557                                                                                                                                                    |                                                                                                                                          atTACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATgccgcc                                                                                                                                         >  2:855015/1‑150 (MQ=255) atTACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGccgcca                                                                                                                                        <  1:240018/151‑1 (MQ=255)   tACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAgg                                                                                                                                      >  2:9236/1‑151 (MQ=255)   tACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAg                                                                                                                                       <  1:34986/150‑1 (MQ=255)           gggAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGa                                                                                                                              <  1:456202/151‑1 (MQ=255)            ggAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACg                                                                                                                               >  1:314084/1‑149 (MQ=255)                gATATCGTGCGGCACCAGCACGGTGGCTGGCGCTGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACg                                                                                                                         >  2:498768/1‑151 (MQ=255)                     cGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCat                                                                                                                     >  1:328835/1‑150 (MQ=255)                     cGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCat                                                                                                                     >  1:852445/1‑150 (MQ=255)                          ggCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGc                                                                                                               >  2:798621/1‑151 (MQ=255)                           gCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGcc                                                                                                              <  1:409980/151‑1 (MQ=255)                                   aCGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCa                                                                                                      <  1:704832/151‑1 (MQ=255)                                      gTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACtt                                                                                                   <  1:61711/151‑1 (MQ=255)                                                     cAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCAt                                                                                    >  2:801212/1‑151 (MQ=255)                                                         tAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGc                                                                                >  2:656281/1‑151 (MQ=255)                                                          aGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGcc                                                                               >  1:348761/1‑151 (MQ=255)                                                                gacgaTACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCagcagc                                                                         >  2:324388/1‑151 (MQ=255)                                                                      aCGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCAc                                                                    <  2:656208/150‑1 (MQ=255)                                                                        gCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCAcgcc                                                                 >  1:138505/1‑151 (MQ=255)                                                                           cGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCcgcg                                                              >  1:624051/1‑151 (MQ=255)                                                                                cGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAg                                                          >  2:698162/1‑150 (MQ=255)                                                                                 ggTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCa                                                        <  1:854956/151‑1 (MQ=255)                                                                                 ggTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAAGGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCa                                                        <  1:759688/151‑1 (MQ=255)                                                                                           tttAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGcc                                              >  2:63984/1‑151 (MQ=255)                                                                                            ttAACGGTTTCAACCAGCAGAGTGCTTTCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGcc                                              <  2:440802/150‑1 (MQ=255)                                                                                                      cAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATc                                   >  2:798776/1‑151 (MQ=255)                                                                                                       aaCCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCg                                  >  2:586869/1‑151 (MQ=255)                                                                                                        aCCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGc                                 <  2:735289/151‑1 (MQ=255)                                                                                                         ccAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGc                                 <  2:430341/150‑1 (MQ=255)                                                                                                         ccAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCt                                <  1:298535/151‑1 (MQ=255)                                                                                                           agcagAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTgg                              <  1:345604/151‑1 (MQ=255)                                                                                                            gcagAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGc                             <  2:352234/151‑1 (MQ=255)                                                                                                             ctgagTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCg                            >  2:842063/3‑151 (MQ=255)                                                                                                              agagTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCg                            >  2:185124/1‑150 (MQ=255)                                                                                                                      aTCGCGAAAACACTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGc                    <  1:472045/150‑1 (MQ=255)                                                                                                                               acacTCCGCGCCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACt          <  1:98796/151‑1 (MQ=255)                                                                                                                                       cgcCTTTAATGCCGCCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATAcc  >  1:74783/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                          ATTACCAATCGGGAAGATATCGTGCGGCACCAGCACGGTGGCTGGCGCGGCGCAAATAGCCGCGACGATACGCCCGGAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACC  >  NC_000913/443273‑443557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑