Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,991,678	T→C	S176P (TCC→CCC)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,991,678	0	T	C	100.0%	185.7 / NA	53	S176P (TCC→CCC)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/28);  total (25/28)

TAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGT  >  NC_000913/3991530‑3991820                                                                                                                                                     |                                                                                                                                               tAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGcccc                                                                                                                                              >  2:451728/1‑151 (MQ=255)  aGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGcccc                                                                                                                                              >  2:42618/1‑150 (MQ=255)     gAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGAGGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCAccc                                                                                                                                          <  2:98484/151‑1 (MQ=255)       agagCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAg                                                                                                                                        <  2:494006/151‑1 (MQ=255)                    cTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACtgctgc                                                                                                                           >  2:800525/1‑151 (MQ=255)                    cTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACtgctgc                                                                                                                           >  1:673850/1‑151 (MQ=255)                      aCAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCtt                                                                                                                         >  2:238288/1‑151 (MQ=255)                       cAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTg                                                                                                                        <  2:561948/151‑1 (MQ=255)                       cAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTg                                                                                                                        <  2:352563/151‑1 (MQ=255)                          cGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACg                                                                                                                     <  2:119482/151‑1 (MQ=255)                          cGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACg                                                                                                                     >  1:50191/1‑151 (MQ=255)                              aaTGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAAtt                                                                                                                 >  2:29189/1‑151 (MQ=255)                                   aGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATc                                                                                                            <  2:120027/151‑1 (MQ=255)                                    gCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCg                                                                                                           <  2:363068/151‑1 (MQ=255)                                    gCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCg                                                                                                           >  2:796448/1‑151 (MQ=255)                                      ctgctgGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGt                                                                                                          >  2:856398/1‑150 (MQ=255)                                       tgctgGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTAc                                                                                                        >  1:527826/1‑151 (MQ=255)                                        gctgGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTAcc                                                                                                       <  2:204130/151‑1 (MQ=255)                                          tgGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTAccgc                                                                                                     >  1:86413/1‑151 (MQ=255)                                           ggAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCTCCCAGCATATACTGCTGCTTGACGAATTTTATCGTAccg                                                                                                      <  1:773798/149‑1 (MQ=255)                                                aCTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCg                                                                                               >  1:708305/1‑151 (MQ=255)                                                   gggCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGtct                                                                                            >  2:443001/1‑151 (MQ=255)                                                    ggCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGtctc                                                                                           <  2:222183/151‑1 (MQ=255)                                                      ccgccTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCg                                                                                          <  2:213381/150‑1 (MQ=255)                                                          cTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGt                                                                                     >  2:792626/1‑151 (MQ=255)                                                             gCTGGTTGTGGAAGCCAGCTTCTCCGTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGCCGAGGATTGTGGCCCCCCCCAGCATATATTGCTTCTTGACGAATGTTATCGTCCCGCCGTGCGTCTCGCCGGTAAg                                                                                  <  1:497939/151‑1 (MQ=255)                                                             gCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTaa                                                                                   <  2:684098/150‑1 (MQ=255)                                                                gggTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGt                                                                               <  1:413187/151‑1 (MQ=255)                                                                 ggTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGt                                                                               >  2:700208/1‑150 (MQ=255)                                                                  gagtgGAAGTCAGCTTCTTCCTAATTGATGAAAACCGCTTCCGTCATAATAACACCGGCAGCCTGGGGTGCGAACATTGTGGCCCCACCCAGCATATCCTGCTGCTTGACGACTTTTATCTTACCGCCGTGCGTCTCGCCGGTAAGc                                                                                 <  1:514884/145‑1 (MQ=255)                                                                      ggAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCtg                                                                         <  2:321996/151‑1 (MQ=255)                                                                             aGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAata                                                                  <  1:211426/151‑1 (MQ=255)                                                                             aGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAata                                                                  <  1:95032/151‑1 (MQ=255)                                                                               cttcttCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATg                                                                >  1:73194/1‑151 (MQ=255)                                                                                   ttcCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGAACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGc                                                            >  2:431436/1‑151 (MQ=255)                                                                                          ttgatGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGcg                                                      >  1:185780/1‑150 (MQ=255)                                                                                                  aaCCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACgaag                                                >  2:661752/1‑148 (MQ=255)                                                                                                   aCCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAagaaga                                            <  1:657174/151‑1 (MQ=255)                                                                                                           cGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTacg                                    >  1:221254/1‑151 (MQ=255)                                                                                                                 aaTGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACt                              <  2:323143/151‑1 (MQ=255)                                                                                                                  aTGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACt                              >  1:279774/1‑150 (MQ=255)                                                                                                                   tGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTAt                            >  1:539215/1‑151 (MQ=255)                                                                                                                    gAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTAt                            <  2:279799/150‑1 (MQ=255)                                                                                                                     aaaGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTAtgt                          >  2:191287/1‑151 (MQ=255)                                                                                                                     aaaGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTAtgt                          >  2:242261/1‑151 (MQ=255)                                                                                                                     aaaGCGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTAtgt                          <  1:796392/151‑1 (MQ=255)                                                                                                                         cGGCAGCCTGGGGGGCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGtgatg                      >  1:715501/1‑151 (MQ=255)                                                                                                                                     gggCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTAcgcg          <  1:636595/151‑1 (MQ=255)                                                                                                                                      ggCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACgcgc         <  2:805528/151‑1 (MQ=255)                                                                                                                                       gCGAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCa        <  1:673296/151‑1 (MQ=255)                                                                                                                                         gAAGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAgg      >  2:644455/1‑151 (MQ=255)                                                                                                                                          aaGATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAggg     <  2:368257/151‑1 (MQ=255)                                                                                                                                            gATTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCg   >  2:252241/1‑151 (MQ=255)                                                                                                                                             aTTGTGGCCCCACCCAGCGTATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTGTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTGCGACGACTATGTGATGACGCTTTACGAGCAGGGCGt  <  1:278067/151‑1 (MQ=255)                                                                                                                                             aTTGTGGCCCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGt  <  2:261470/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               TAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATGTGATGACGCTTTACGCGCAGGGCGT  >  NC_000913/3991530‑3991820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑