Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,286,249	T→C	T375A (ACC→GCC)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,286,249	0	T	C	100.0%	151.8 / NA	44	T375A (ACC→GCC)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

GATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGG  >  NC_000913/4286103‑4286395                                                                                                                                                   |                                                                                                                                                   gATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCt                                                                                                                                                >  2:719974/1‑151 (MQ=255) gATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCt                                                                                                                                                >  2:260352/1‑151 (MQ=255)        aaaCCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGAt                                                                                                                                           <  1:79172/149‑1 (MQ=255)          aCCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCt                                                                                                                                       >  1:33000/1‑151 (MQ=255)                       gTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCg                                                                                                                          <  2:657327/151‑1 (MQ=255)                           gCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCAt                                                                                                                      >  2:501077/1‑151 (MQ=255)                             caccaGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCa                                                                                                                    <  2:791288/151‑1 (MQ=255)                              accaGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAg                                                                                                                   <  1:496641/151‑1 (MQ=255)                               ccaGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAgc                                                                                                                  <  2:466272/151‑1 (MQ=255)                               ccaGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAg                                                                                                                   <  2:677587/150‑1 (MQ=255)                                     aTCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGcgcgcg                                                                                                             <  2:681024/150‑1 (MQ=255)                                       cGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGAt                                                                                                          <  1:519051/151‑1 (MQ=255)                                        gACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATc                                                                                                         >  2:341248/1‑151 (MQ=255)                                        gACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATc                                                                                                         >  1:761215/1‑151 (MQ=255)                                        gACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATc                                                                                                         <  2:753269/151‑1 (MQ=255)                                              cGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTg                                                                                                   <  2:530586/151‑1 (MQ=255)                                               ggACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTgg                                                                                                  <  1:722069/151‑1 (MQ=255)                                               ggACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTgg                                                                                                  <  1:341225/151‑1 (MQ=255)                                                    tttCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCg                                                                                             >  1:786928/1‑151 (MQ=255)                                                       ctcGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGt                                                                                           >  2:15102/1‑150 (MQ=255)                                                         cGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGtat                                                                                         <  1:735679/150‑1 (MQ=255)                                                          gTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGtat                                                                                         >  1:82156/1‑149 (MQ=255)                                                                      tttCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGAc                                                                            <  1:731149/150‑1 (MQ=255)                                                                          cAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGAt                                                                       >  1:713646/1‑151 (MQ=255)                                                                           aGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATg                                                                      >  2:197865/1‑151 (MQ=255)                                                                           aGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATg                                                                      <  2:458227/151‑1 (MQ=255)                                                                                   tCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcc                                                               >  1:366601/1‑150 (MQ=255)                                                                                    cccACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTccac                                                             >  2:243447/1‑151 (MQ=255)                                                                                    cccACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcc                                                               <  2:185802/149‑1 (MQ=255)                                                                                         gCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCt                                                        <  1:133802/151‑1 (MQ=255)                                                                                            tCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTg                                                       <  1:788121/149‑1 (MQ=255)                                                                                              cGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc                                                   >  1:555556/1‑151 (MQ=255)                                                                                                ggTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCAt                                                 <  1:165234/151‑1 (MQ=255)                                                                                                ggTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCAt                                                 <  1:197842/151‑1 (MQ=255)                                                                                                   tAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACg                                              >  1:5125/1‑151 (MQ=255)                                                                                                         gCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAgg                                        >  1:350209/1‑151 (MQ=255)                                                                                                                    gAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGt                             <  1:480968/151‑1 (MQ=255)                                                                                                                     aaCCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGtt                            >  1:323395/1‑151 (MQ=255)                                                                                                                       ccGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTgg                          <  2:713675/151‑1 (MQ=255)                                                                                                                          agaATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTa                       <  1:29024/151‑1 (MQ=255)                                                                                                                           gaATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTAc                      >  2:668655/1‑151 (MQ=255)                                                                                                                                      gacgaACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAAc            >  2:705459/1‑150 (MQ=255)                                                                                                                                       acgaACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACAt          >  1:738394/1‑151 (MQ=255)                                                                                                                                           aCGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCACACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGc      >  1:514117/1‑151 (MQ=255)                                                                                                                                              gtcggcGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTg   <  2:80033/151‑1 (MQ=255)                                                                                                                                                cggcGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTgg  <  2:310162/150‑1 (MQ=255)                                                                                                                                                cggcGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTgg  <  2:748274/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGG  >  NC_000913/4286103‑4286395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑