Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,457,566 C→T A100A (GCG→GCA)  yjgA ← ribosome‑associated UPF0307 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,457,5660CT98.0% 179.3 / NA 50A100A (GCG→GCA) yjgAribosome‑associated UPF0307 family protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (28/21);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAGCGCGT  >  NC_000913/4457420‑4457711
                                                                                                                                                  |                                                                                                                                                 
cGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTACTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCt                                                                                                                                               <  2:192707/151‑1 (MQ=255)
       gCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAAt                                                                                                                                        >  1:737334/1‑151 (MQ=255)
         tGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAAt                                                                                                                                        >  2:77875/1‑149 (MQ=255)
            cGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGc                                                                                                                                    <  1:299147/150‑1 (MQ=255)
               tCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGctct                                                                                                                                 >  2:804280/1‑150 (MQ=255)
                 ggCATCGGGCCACAGATTTAATCCCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATGGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGTTTGTGACGG‑TTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGt                                                                                                                             <  1:503226/151‑1 (MQ=255)
                   cATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTc                                                                                                                            <  2:552699/151‑1 (MQ=255)
                       gggCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCgcgc                                                                                                                        <  1:277563/151‑1 (MQ=255)
                          ccACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTgg                                                                                                                     <  2:756984/151‑1 (MQ=255)
                            acaGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGgcg                                                                                                                   >  2:636925/1‑151 (MQ=255)
                                     aTACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCCGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCt                                                                                                           >  1:794824/1‑150 (MQ=255)
                                       aCCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTAc                                                                                                        >  2:381699/1‑151 (MQ=255)
                                          tCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCg                                                                                                      >  2:476790/1‑150 (MQ=255)
                                             gCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATg                                                                                                   >  2:485863/1‑150 (MQ=255)
                                             gCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGa                                                                                                  <  2:154783/151‑1 (MQ=255)
                                                  ggCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGGTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGGCGCGCTGGCGCAGCATCTTACCGATGAGCTGc                                                                                             >  2:636962/1‑151 (MQ=255)
                                                   gCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCa                                                                                            <  1:223045/151‑1 (MQ=255)
                                                     gTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAAc                                                                                          <  1:15272/151‑1 (MQ=255)
                                                        atcaCCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTgg                                                                                       >  1:695103/1‑151 (MQ=255)
                                                           aCCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTggcgg                                                                                    >  1:561966/1‑151 (MQ=255)
                                                            ccTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATGCAGTGCCTGACGAATAGGCTCTTCGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTggctgc                                                                                   <  1:327545/151‑1 (MQ=255)
                                                                gatcgatcAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGgcggcggcg                                                                               >  2:429239/1‑151 (MQ=255)
                                                                   cgatcAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGAcc                                                                            >  2:315451/1‑151 (MQ=255)
                                                                   cgatcAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGAcc                                                                            <  2:715934/151‑1 (MQ=255)
                                                                       cAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTc                                                                         <  2:202638/150‑1 (MQ=255)
                                                                         gACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCAt                                                                      >  2:95471/1‑151 (MQ=255)
                                                                          aCGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATc                                                                     >  2:635745/1‑151 (MQ=255)
                                                                            aaTCGCGCAGGATTTCTAGTTTATGGAAGAGCACCACCCGCTGGTTGTGACGGAATTTCAGCTTATCCAGCGCCTGACGAATAGTCTCTACGTCGCGCTGGCCCAGCATCTTCCCGTTGACCTGCAACTGGCGGCGGCGACCTTCCATc                                                                     >  1:706815/2‑149 (MQ=255)
                                                                               cgcgCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAAt                                                                <  2:782059/151‑1 (MQ=255)
                                                                                         ttCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGc                                                       <  1:369187/150‑1 (MQ=255)
                                                                                           ccAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCg                                                      >  1:68997/1‑149 (MQ=255)
                                                                                            cAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGc                                                   >  2:734270/1‑151 (MQ=255)
                                                                                                ttATGGAACAGCACCACCTGCTGGTTGTGACGGCTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCAATGAGCTGCAACTGGCGGCGGCGACCTTCCCTCTTAATACGCTGGGCGAGCTCa                                                >  1:201769/1‑150 (MQ=255)
                                                                                                  aTGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAAt                                              <  2:206807/150‑1 (MQ=255)
                                                                                                  aTGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAAt                                              >  2:252037/1‑150 (MQ=255)
                                                                                                  aTGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATa                                             <  2:700257/151‑1 (MQ=255)
                                                                                                     gAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGcc                                          <  2:235975/151‑1 (MQ=255)
                                                                                                          gCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCcgcgcg                                     <  1:216450/151‑1 (MQ=255)
                                                                                                                ccTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCgcgcgc                                    >  2:344602/1‑146 (MQ=255)
                                                                                                                ccTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCgcgcgc                                    >  2:220157/1‑146 (MQ=255)
                                                                                                                  tgctgGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCg                             >  1:200632/1‑151 (MQ=255)
                                                                                                                          gtgACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTaa                      <  1:175764/150‑1 (MQ=255)
                                                                                                                             aCGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACggg                  >  2:384631/1‑151 (MQ=255)
                                                                                                                                gTTTTTCAGCTTATCCAGTGCCTGACGTATCGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGCCCTTCCATCTTAATACGTTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATc               >  1:429804/1‑151 (MQ=255)
                                                                                                                                          ttATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAgcg     >  1:815608/1‑151 (MQ=255)
                                                                                                                                           tATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAgcgc    >  1:250894/1‑151 (MQ=255)
                                                                                                                                           tATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAgcg     <  1:635703/150‑1 (MQ=255)
                                                                                                                                           tATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAgc      <  1:350389/149‑1 (MQ=255)
                                                                                                                                            aTCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAGcgcg   >  1:183409/1‑151 (MQ=255)
                                                                                                                                             tCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAGCGCGt  >  1:378088/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAGCGCGT  >  NC_000913/4457420‑4457711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑