Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,550,166	A→G	T377T (ACT→ACC)	gntP ←	fructuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,550,166	0	A	G	100.0%	152.2 / NA	43	T377T (ACT→ACC)	gntP	fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/25);  total (18/25)

GGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAAC  >  NC_000913/4550025‑4550307                                                                                                                                              |                                                                                                                                              ggTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTgg                                                                                                                                       >  2:505059/1‑150 (MQ=255)  gTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGAt                                                                                                                                     >  2:172076/1‑151 (MQ=255)         aCGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGa                                                                                                                               >  1:498952/1‑150 (MQ=255)          cGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGAtt                                                                                                                             <  1:543827/151‑1 (MQ=255)                 tttACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCAc                                                                                                                      >  1:566231/1‑151 (MQ=255)                 tttACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCAc                                                                                                                      >  2:221916/1‑151 (MQ=255)                 tttACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCAc                                                                                                                      >  2:497319/1‑151 (MQ=255)                      tGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGAt                                                                                                                  >  1:365661/1‑150 (MQ=255)                        aCAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAAt                                                                                                               >  1:236426/1‑151 (MQ=255)                            gTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCcg                                                                                                           <  2:61135/151‑1 (MQ=255)                               aaaGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTgca                                                                                                                          <  2:304968/133‑1 (MQ=255)                               aaaGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTgca                                                                                                                          >  1:304943/1‑133 (MQ=255)                               aaaGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCcggcg                                                                                                        <  2:56548/151‑1 (MQ=255)                                    aaCCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCcggcggcgg                                                                                                    <  1:588247/150‑1 (MQ=255)                                         ttGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCg                                                                                              >  1:694344/1‑151 (MQ=255)                                            aacaacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCg                                                                                           >  1:591356/1‑151 (MQ=255)                                              caacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGaa                                                                                         <  1:193277/151‑1 (MQ=255)                                              caacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGa                                                                                          >  1:497207/1‑150 (MQ=255)                                               aacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGaaa                                                                                        <  2:485109/151‑1 (MQ=255)                                                           gCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGAc                                                                            >  2:30351/1‑151 (MQ=255)                                                             aTCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGAccc                                                                          <  2:666401/151‑1 (MQ=255)                                                               cGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGt                                                                        <  2:185563/151‑1 (MQ=255)                                                                gTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTc                                                                       <  1:5959/151‑1 (MQ=255)                                                                      gtgGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAg                                                                  <  1:497283/150‑1 (MQ=255)                                                                            gAGGGTGTTGGAATCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAAt                                                            <  1:127553/150‑1 (MQ=255)                                                                                       aaCCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGAt                                                <  2:110457/151‑1 (MQ=255)                                                                                        aCCCGCAGCCGTCGCCAGTACCAGCAGCACCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGAt                                                <  1:672226/150‑1 (MQ=255)                                                                                          ccGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATc                                               >  1:314029/1‑149 (MQ=255)                                                                                                    cGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgccat                                    <  1:453231/150‑1 (MQ=255)                                                                                                    cGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGccatgccatg                                   >  2:295388/1‑151 (MQ=255)                                                                                                    cGCCAGTACCAGCAGCGACGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgccat                                    <  2:114399/150‑1 (MQ=255)                                                                                                      ccAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCatgat                                 <  1:161670/151‑1 (MQ=255)                                                                                                       cAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCatgatg                                <  2:255138/151‑1 (MQ=255)                                                                                                           aCCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAgg                            >  2:492635/1‑151 (MQ=255)                                                                                                                 agcGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCg                         >  2:167856/1‑148 (MQ=255)                                                                                                                  gcgcCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGat                     <  2:486939/151‑1 (MQ=255)                                                                                                                     ccGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATAtt                  <  2:515333/151‑1 (MQ=255)                                                                                                                       ggATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGc                <  1:1630/151‑1 (MQ=255)                                                                                                                       ggATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGc                <  2:764309/151‑1 (MQ=255)                                                                                                                                ccAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGAc        >  1:669547/1‑150 (MQ=255)                                                                                                                                ccAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACa       <  2:23846/151‑1 (MQ=255)                                                                                                                                   aCCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCa    <  1:408823/151‑1 (MQ=255)                                                                                                                                     cAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGCCATCGCCGAAACGACACCCTGACCCGTCGCCAGACTAATTAGCACAGTGATCCGCCATGCCATGATGTAGCGCGTGATATTGCCGTGGGACATCacc  <  1:57829/151‑3 (MQ=255)                                                                                                                                              |                                                                                                                                              GGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAAC  >  NC_000913/4550025‑4550307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑