Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,608,413	C→T	R77C (CGT→TGT)	rimI →	ribosomal‑protein‑S18‑alanine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,608,413	0	C	T	100.0%	274.1 / NA	73	R77C (CGT→TGT)	rimI	ribosomal‑protein‑S18‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/31);  total (42/31)

AGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGCGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACG  >  NC_000913/4608266‑4608556                                                                                                                                                    |                                                                                                                                                aGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTc                                                                                                                                              <  2:503095/151‑1 (MQ=255)   tGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTc                                                                                                                                              >  1:822090/1‑149 (MQ=255)    gAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAgg                                                                                                                                           >  2:554739/1‑151 (MQ=255)     aaaaaaCGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAgg                                                                                                                                           <  2:699607/150‑1 (MQ=255)      aaaaaCGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAggg                                                                                                                                          >  1:265481/1‑150 (MQ=255)        aaaCGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGAtt                                                                                                                                       <  1:176111/151‑1 (MQ=255)          aCGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTgg                                                                                                                                     <  2:351889/151‑1 (MQ=255)           cGTTTGCCAGCATCCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTggt                                                                                                                                    <  1:264702/151‑2 (MQ=255)                gCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAgg                                                                                                                                <  2:642890/150‑1 (MQ=255)                 ccAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGgc                                                                                                                              <  1:714549/151‑1 (MQ=255)                    gCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCgct                                                                                                                           <  2:858811/151‑1 (MQ=255)                     cAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCgctg                                                                                                                          >  1:18020/1‑151 (MQ=255)                      aaCCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCgctgc                                                                                                                         >  1:53939/1‑151 (MQ=255)                        ccAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGctgctg                                                                                                                       <  1:588459/151‑1 (MQ=255)                              cGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACAt                                                                                                                 >  1:161204/1‑151 (MQ=255)                              cGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACAt                                                                                                                 >  1:64307/1‑151 (MQ=255)                                aGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCt                                                                                                               >  2:389885/1‑151 (MQ=255)                                  cGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGa                                                                                                             <  2:396010/151‑1 (MQ=255)                                    ttATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAgg                                                                                                                                <  1:526391/130‑1 (MQ=255)                                    ttATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAgg                                                                                                                                >  2:526429/1‑130 (MQ=255)                                     tATCTCAACTTTCAGTTAACGCAAAACGGCAAACTGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATc                                                                                                           >  1:745585/1‑150 (MQ=255)                                     tATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATc                                                                                                           <  2:587500/150‑1 (MQ=255)                                      aTCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATcg                                                                                                          >  2:114863/1‑150 (MQ=255)                                      aTCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATcg                                                                                                          >  1:703504/1‑150 (MQ=255)                                       tctcAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATcgac                                                                                                        >  2:93935/1‑151 (MQ=255)                                        ctcAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATcgacg                                                                                                       >  2:80820/1‑151 (MQ=255)                                          cAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATcgacga                                                                                                      <  1:347036/150‑1 (MQ=255)                                                  aGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGaaa                                                                                              >  2:313840/1‑150 (MQ=255)                                                   gTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGaaaaa                                                                                            <  1:610756/151‑1 (MQ=255)                                                    ttAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAc                                                                                           >  1:829562/1‑151 (MQ=255)                                                    ttAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAc                                                                                           >  2:99102/1‑151 (MQ=255)                                                     tAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAc                                                                                           <  2:188259/150‑1 (MQ=255)                                                      aaCGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAcg                                                                                          <  1:620796/150‑1 (MQ=255)                                                       aCGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAcgcg                                                                                        >  1:373509/1‑151 (MQ=255)                                                         gCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACgcggc                                                                                      >  1:565292/1‑151 (MQ=255)                                                         gCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACgcggc                                                                                      >  2:783512/1‑151 (MQ=255)                                                             aaCGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTgg                                                                                  >  1:45640/1‑151 (MQ=255)                                                                ggCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGa                                                                               <  1:819717/151‑1 (MQ=255)                                                                     aaTGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACt                                                                           >  1:5850/1‑150 (MQ=255)                                                                       tGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATg                                                                        >  1:453441/1‑151 (MQ=255)                                                                        ggcggcGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATgg                                                                       >  2:95663/1‑151 (MQ=255)                                                                        ggcggcGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATgg                                                                       >  2:79746/1‑151 (MQ=255)                                                                         gcggcgTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGc                                                                      <  1:114840/151‑1 (MQ=255)                                                                          cggcgTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATggat                                                                     <  2:298066/151‑3 (MQ=255)                                                                            gcgTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTgg                                                                   >  2:600664/1‑151 (MQ=255)                                                                              gTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGaa                                                                 <  1:285930/151‑1 (MQ=255)                                                                                ttGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGt                                                               >  2:605488/1‑151 (MQ=255)                                                                                  gCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTcc                                                             >  2:610642/1‑151 (MQ=255)                                                                                  gCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTcc                                                             <  1:545234/151‑1 (MQ=255)                                                                                   cGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCg                                                            >  1:218541/1‑151 (MQ=255)                                                                                    gATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGt                                                           >  1:344617/1‑151 (MQ=255)                                                                                      ttACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGc                                                         >  1:706389/1‑151 (MQ=255)                                                                                            aaGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCaaa                                                   <  2:351848/151‑1 (MQ=255)                                                                                               tggtgCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGc                                                >  2:264307/1‑151 (MQ=255)                                                                                                ggtgCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGc                                                <  2:662590/150‑1 (MQ=255)                                                                                                     tGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTgccgc                                          >  2:706361/1‑151 (MQ=255)                                                                                                      ggATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTgccgcc                                         >  2:597215/1‑151 (MQ=255)                                                                                                                aCATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCt                                 <  1:161837/149‑1 (MQ=255)                                                                                                                 cATTGTTCAATATTGCTGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTa                              <  2:200416/151‑1 (MQ=255)                                                                                                                   ttGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACg                            >  2:217544/1‑151 (MQ=255)                                                                                                                    tGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACg                            <  1:442650/150‑1 (MQ=255)                                                                                                                     gTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGa                           >  1:360718/1‑150 (MQ=255)                                                                                                                        cAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAg                        <  1:823869/150‑1 (MQ=255)                                                                                                                         aaTATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGtt                      <  2:161227/151‑1 (MQ=255)                                                                                                                           tatTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGttt                     >  2:628302/1‑150 (MQ=255)                                                                                                                           tatTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGttt                     >  2:335838/1‑150 (MQ=255)                                                                                                                                cGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCtt               <  2:218565/151‑1 (MQ=255)                                                                                                                                  gTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCttt              >  2:801020/1‑150 (MQ=255)                                                                                                                                  gTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTa             <  1:432662/151‑1 (MQ=255)                                                                                                                                    cGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTaa            <  2:207746/150‑1 (MQ=255)                                                                                                                                    cGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAAc           >  2:783275/1‑151 (MQ=255)                                                                                                                                          tGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGcg     >  1:741832/1‑151 (MQ=255)                                                                                                                                              tATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGcgacg  >  2:296508/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                AGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGCGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACG  >  NC_000913/4608266‑4608556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑