Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 514,375 | G→A | G128D (GGC→GAC) | cueR → | copper‑responsive regulon transcriptional regulator |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 514,375 | 0 | G | A | 98.3% | 217.8 / NA | 59 | G128D (GGC→GAC) | cueR | copper‑responsive regulon transcriptional regulator |
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (24/34); minor base T (1/0); total (25/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.24e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCG > NC_000913/514229‑514517 | cGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGactgc > 2:113816/1‑151 (MQ=255) cGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGactg < 1:678759/150‑1 (MQ=255) gTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGactgct < 2:249194/151‑1 (MQ=255) cAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGActgctg < 1:499272/150‑1 (MQ=255) cAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGt > 1:451048/1‑151 (MQ=255) aCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGtcat < 1:294053/151‑1 (MQ=255) cgcACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCatcatc > 1:529006/1‑151 (MQ=255) ggAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAgg < 2:451082/150‑1 (MQ=255) gagaAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAggg > 1:415055/1‑150 (MQ=255) gagaAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGt < 2:811407/151‑1 (MQ=255) aGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCTCTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGAtt < 1:260875/151‑1 (MQ=255) ggAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAagacga < 2:246893/151‑1 (MQ=255) gaTCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAagacgagac < 2:134506/151‑1 (MQ=255) aTCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAagacgagacg < 1:104446/151‑1 (MQ=255) cGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGc > 1:114824/1‑151 (MQ=255) gAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCt < 2:839912/151‑1 (MQ=255) gAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCt < 1:535739/151‑1 (MQ=255) acacATTGAGGAGCTGCGATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCTGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGat < 1:573754/151‑1 (MQ=255) cacaTTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGat > 2:689810/1‑150 (MQ=255) ggagCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGt < 2:506857/151‑1 (MQ=255) agCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGtt > 2:732310/1‑150 (MQ=255) aaTCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCtt < 1:707684/151‑1 (MQ=255) aTCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTc > 1:820675/1‑151 (MQ=255) ccATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTa < 2:114715/151‑1 (MQ=255) gcgcGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCg < 1:28474/150‑1 (MQ=255) cAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGATGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACt > 1:433881/1‑151 (MQ=255) tgctgGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCa > 1:633107/1‑150 (MQ=255) tgctgGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCa > 2:716447/1‑150 (MQ=255) gctgGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCaa < 2:725702/150‑1 (MQ=255) ctgGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAAc > 1:670158/1‑150 (MQ=255) aCTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGt < 2:117559/151‑1 (MQ=255) tcgcgaccgcctgccGTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCCCCAACTGCTGTCATCATCAGGAGGGGTGATTAAGACGAGCCGGCTCGGATATGTAGGGTTATCCCTTCCATCGCCATGACTTCAACATGCGCAc > 1:330220/9‑151 (MQ=255) cGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACATg > 1:334185/1‑151 (MQ=255) aaTGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCa < 1:625235/151‑1 (MQ=255) aaTGACTGCCCTGGCGATGACCGCGCCGACTGCTCGATTACCGCAAATCTCTGCGTCTGCGGTTATCTTTGGGCAGGGTGATTAAGACGAGTGGGCTCGGGTACGTAGGGTTAGCCCTTATATCGCACTGACTTCAACATGCGTAcc > 1:188339/1‑147 (MQ=255) gcctgccCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCAcc < 1:235164/150‑1 (MQ=255) tgccCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCg > 1:729130/1‑148 (MQ=255) gccCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGAt < 1:113793/151‑1 (MQ=255) ggCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTc < 1:28073/150‑1 (MQ=255) gATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTg < 1:500155/151‑1 (MQ=255) aTGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTgg < 1:810500/151‑1 (MQ=255) gcagACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCCGCACCGAGATCCTCGCTGGCGCTGACa < 2:630293/148‑1 (MQ=255) cGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAgg > 1:307138/1‑151 (MQ=255) cGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAgg < 2:729173/151‑1 (MQ=255) cGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAgg < 1:672628/151‑1 (MQ=255) aCTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGcc > 2:799813/1‑151 (MQ=255) aCTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGcc < 2:777781/151‑1 (MQ=255) gCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATg > 1:694943/1‑151 (MQ=255) cccGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGa < 2:636551/151‑1 (MQ=255) ccGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGa > 1:179296/1‑150 (MQ=255) cGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAAc > 2:257220/1‑151 (MQ=255) gATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACt < 1:632781/151‑1 (MQ=255) aTTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTg > 1:378039/1‑151 (MQ=255) tATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTc > 2:438047/1‑151 (MQ=255) aTCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTc > 1:103227/1‑150 (MQ=255) cGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCAc < 1:723915/150‑1 (MQ=255) cGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCAc < 1:442400/150‑1 (MQ=255) aaaaTCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCg > 2:565425/1‑150 (MQ=255) aaaTCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGAc < 1:640078/151‑1 (MQ=255) aaaTCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGAc < 1:721881/151‑1 (MQ=255) aTCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGAcgc > 2:217939/1‑151 (MQ=255) ctcCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGAcgcg < 1:62734/148‑1 (MQ=255) | CGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCG > NC_000913/514229‑514517 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |