Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	541,599	A→G	L345L (TTA→TTG)	ybbY →	putative uracil/xanthine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	541,599	0	A	G	100.0%	186.2 / NA	53	L345L (TTA→TTG)	ybbY	putative uracil/xanthine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/30);  total (23/30)

TCCATTTTCACCGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATGGCATTAC  >  NC_000913/541455‑541744                                                                                                                                                 |                                                                                                                                                  tCCATTTTCACCGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGt                                                                                                                                              <  1:125518/150‑1 (MQ=255)    aTTTTCACCGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGagt                                                                                                                                          <  2:51010/151‑1 (MQ=255)      tttCACCGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGagt                                                                                                                                          >  2:513575/1‑149 (MQ=255)      tttCACCGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATGTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCCCGCGACTGTTTTGCAGTATCCCTTTGCCCGTGagta                                                                                                                                         <  2:676700/150‑1 (MQ=255)        tCACCGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGagtagt                                                                                                                                       <  2:49947/150‑1 (MQ=255)         cACCGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTg                                                                                                                                      <  1:753084/150‑1 (MQ=255)          aCCGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGTGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGc                                                                                                                                     >  1:86666/1‑150 (MQ=255)           ccGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCgg                                                                                                                                   >  1:347412/1‑151 (MQ=255)              tttgtttCATCCATTTGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTc                                                                                                                                 >  2:378279/1‑150 (MQ=255)                tgtttCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCAt                                                                                                                               <  1:472303/150‑1 (MQ=255)                 gtttCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGTCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGc                                                                                                                             <  2:856996/151‑1 (MQ=255)                     cATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGt                                                                                                                         <  2:220394/151‑1 (MQ=255)                      aTCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGtt                                                                                                                        <  1:403316/151‑1 (MQ=255)                       tCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGttt                                                                                                                       >  1:460017/1‑151 (MQ=255)                        ccATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTc                                                                                                                      >  1:835015/1‑151 (MQ=255)                         cATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCt                                                                                                                     <  1:542597/151‑1 (MQ=255)                          aTTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCtt                                                                                                                    >  1:530460/1‑151 (MQ=255)                                    taACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCtt                                                                                                           <  1:471118/150‑1 (MQ=255)                                               ggCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTcc                                                                                                <  1:483592/150‑1 (MQ=255)                                                  gATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCgcgc                                                                                            >  2:386417/1‑151 (MQ=255)                                                      acacGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGgt                                                                                        <  2:306834/151‑1 (MQ=255)                                                        acGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGgtgt                                                                                      <  2:302185/151‑1 (MQ=255)                                                            ggcgTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGttt                                                                                    >  1:848004/1‑149 (MQ=255)                                                             gcgTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGc                                                                                 >  1:617121/1‑151 (MQ=255)                                                                  cgttTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGc                                                                             >  2:708742/1‑150 (MQ=255)                                                                   gttTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCa                                                                            >  2:631647/1‑150 (MQ=255)                                                                   gttTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCa                                                                            >  2:132019/1‑150 (MQ=255)                                                                     ttatttatGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCaaa                                                                          <  1:170796/150‑1 (MQ=255)                                                                          aatGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCAATCACGCGACTGTTTTGCAGTATCCCTGTGCCCGGGGGTAGTGCGGACATGCTGGTTTATTATCGGCCTTTACTCTTTTCCGAGCTGGTGTTTAGCCAGCAAATAACg                                                                     >  1:458994/2‑150 (MQ=255)                                                                           atGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGtt                                                                   >  2:337473/1‑151 (MQ=255)                                                                                       tttGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCa                                                        >  1:319058/1‑150 (MQ=255)                                                                                       tttGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCa                                                        >  2:681326/1‑150 (MQ=255)                                                                                              gctgGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTAt                                                <  2:684237/151‑1 (MQ=255)                                                                                               ctgGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTAt                                                <  1:26565/150‑1 (MQ=255)                                                                                                  gtggCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGAc                                            >  2:152493/1‑151 (MQ=255)                                                                                                     gcgcTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCg                                         <  2:600826/151‑1 (MQ=255)                                                                                                      cgcTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGc                                        <  2:345054/151‑1 (MQ=255)                                                                                                         tGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCAtt                                     >  2:108528/1‑151 (MQ=255)                                                                                                          ggTTCCTGCGCTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCAtt                                     <  2:460053/150‑1 (MQ=255)                                                                                                          ggTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCAt                                      <  1:337450/149‑1 (MQ=255)                                                                                                          ggTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTg                                    <  1:88748/151‑1 (MQ=255)                                                                                                              ccTGCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGt                                >  2:127828/1‑151 (MQ=255)                                                                                                                 gCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGtt                               <  1:117603/149‑1 (MQ=255)                                                                                                                 gCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTa                              <  1:169424/150‑1 (MQ=255)                                                                                                                 gCACTCACGCGACTGTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTAt                             <  2:782296/151‑1 (MQ=255)                                                                                                                               gTTTTGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATAttt               <  1:587889/151‑1 (MQ=255)                                                                                                                                   tGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTa            >  1:269368/1‑150 (MQ=255)                                                                                                                                   tGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTa            <  1:286610/150‑1 (MQ=255)                                                                                                                                   tGCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTa            >  1:475603/1‑150 (MQ=255)                                                                                                                                    gCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTaa           <  1:499316/150‑1 (MQ=255)                                                                                                                                    gCAGTATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAAt          <  1:266875/151‑1 (MQ=255)                                                                                                                                        tATCCCTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATGGc       >  1:430348/1‑150 (MQ=255)                                                                                                                                            ccTTTGCCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATGGCATTAc  <  2:242912/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  TCCATTTTCACCGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTTATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTTTGCAGTATCCCTTTACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTCTTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTCGCATTGCCGTTATTTGTCGGCATATTTTTAATGGCATTAC  >  NC_000913/541455‑541744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑