Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	548,053	A→G	E458E (GAA→GAG)	fdrA →	putative NAD(P)‑binding acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	548,053	0	A	G	100.0%	187.1 / NA	50	E458E (GAA→GAG)	fdrA	putative NAD(P)‑binding acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/13);  total (37/13)

CGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTC  >  NC_000913/547912‑548196                                                                                                                                              |                                                                                                                                                cgTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGAccc                                                                                                                                         >  1:422293/1‑150 (MQ=255) cgTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCg                                                                                                                                        >  2:336134/1‑151 (MQ=255)  gtgtTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGc                                                                                                                                       >  1:527057/1‑151 (MQ=255)      tgctgcTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATg                                                                                                                                   >  1:711277/1‑151 (MQ=255)          gctTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCg                                                                                                                                <  2:247129/150‑1 (MQ=255)             tGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCg                                                                                                                            >  2:389340/1‑151 (MQ=255)                  tCGTGATTGGCTTGGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCgcagc                                                                                                                        <  1:136374/150‑1 (MQ=255)                       aTTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCg                                                                                                                  >  1:161614/1‑151 (MQ=255)                         tGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGcc                                                                                                                >  1:721939/1‑151 (MQ=255)                          ggCCTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCg                                                                                                                  <  1:303919/148‑1 (MQ=255)                                     gACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGa                                                                                                        >  1:175197/1‑147 (MQ=255)                                     gACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGCATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGa                                                                                                        >  1:859059/1‑147 (MQ=255)                                          ccgATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCggg                                                                                               >  1:40081/1‑151 (MQ=255)                                             aTCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGAt                                                                                            <  1:251580/151‑1 (MQ=255)                                             aTCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGAt                                                                                            <  2:739214/151‑1 (MQ=255)                                                            tGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTc                                                                             >  1:369988/1‑151 (MQ=255)                                                                    gcCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCgg                                                                     <  2:303197/151‑1 (MQ=255)                                                                       tGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGa                                                                    >  1:66750/1‑149 (MQ=255)                                                                            aaaaGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCAcc                                                             >  2:46947/1‑151 (MQ=255)                                                                                gCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGc                                                         >  2:361471/1‑151 (MQ=255)                                                                                 ccTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCt                                                        <  2:705243/151‑1 (MQ=255)                                                                                     tgCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTggcg                                                    >  1:438299/1‑151 (MQ=255)                                                                                     tgCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTggcg                                                    >  2:11439/1‑151 (MQ=255)                                                                                         gcgcgTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGCCGTGAGTTCGCTACCGGAAGCCACCTCGCTggcggcgg                                                >  1:443729/1‑149 (MQ=255)                                                                                          cgcgTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGc                                               >  2:491462/1‑151 (MQ=255)                                                                                          cgcgTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGc                                               >  1:103490/1‑151 (MQ=255)                                                                                              tttAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGtt                                            >  2:684939/1‑150 (MQ=255)                                                                                               ttAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTa                                           <  2:230134/150‑1 (MQ=255)                                                                                               ttAGATAATCAACCACTGGATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGCCGGCAGCGTTa                                           >  1:115919/1‑150 (MQ=255)                                                                                                tAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTaa                                          <  1:538283/150‑1 (MQ=255)                                                                                                tAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAAt                                         >  2:718304/1‑151 (MQ=255)                                                                                                  gATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTc                                       >  1:800780/1‑151 (MQ=255)                                                                                                    tAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGTGGCAGCGTTAATTCa                                      >  2:633888/1‑150 (MQ=255)                                                                                                      aTCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATcc                                   <  1:436222/151‑1 (MQ=255)                                                                                                        cAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGc                                 >  2:319890/1‑151 (MQ=255)                                                                                                        cAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGc                                 >  2:568879/1‑151 (MQ=255)                                                                                                          aCCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCTCCTTGCTGGCGGCAGCGTTAATTCATCCg                                  <  1:240451/148‑1 (MQ=255)                                                                                                          aCCACTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGctc                               >  1:168001/1‑151 (MQ=255)                                                                                                            cACCGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCtctc                             >  1:117331/1‑151 (MQ=255)                                                                                                             aCTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCtctc                             >  1:292781/1‑150 (MQ=255)                                                                                                             aCTGTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCtctc                             <  2:292806/150‑1 (MQ=255)                                                                                                                gTATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCt                         >  1:793047/1‑151 (MQ=255)                                                                                                                 tATGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTg                        >  2:6306/1‑151 (MQ=255)                                                                                                                   tGCCATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGcc                      >  2:780282/1‑151 (MQ=255)                                                                                                                     ccATTGCCACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGc                    >  1:125598/1‑151 (MQ=255)                                                                                                                            cACGGTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCa              >  2:79681/1‑150 (MQ=255)                                                                                                                              cGGTGACAGGCACTGGGCGTGACCGGCAATGCCACTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCATGCCGCAAAGCAac            <  1:254233/150‑1 (MQ=255)                                                                                                                               ggTGACAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAacac          >  2:193399/1‑151 (MQ=255)                                                                                                                                   aCAGGCACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAacacacac      >  2:390756/1‑151 (MQ=255)                                                                                                                                        cACTGAGCGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTc  >  2:69371/1‑150 (MQ=255)                                                                                                                                              |                                                                                                                                                CGTGTTGCTGCTTGATGTCGTGATTGGCTTCGGTGCGACCGCCGATCCTGCCGCCTCGCTGGTGAGCGCCTGGCAAAAAGCCTGTGCCGCGCGTTTAGATAATCAACCACTGTATGCCATTGCCACGGTGACAGGCACTGAACGTGACCCGCAATGCCGCTCGCAGCAAATCGCCACGCTGGAAGATGCGGGGATTGCGGTCGTGAGTTCGCTACCGGAAGCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTC  >  NC_000913/547912‑548196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑