Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 663,381 C→T A195T (GCG→ACG)  dacA ← D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913663,3810CT98.6% 253.5 / NA 69A195T (GCG→ACG) dacAD‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (42/26);  total (43/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGATGCCGTCGACATTCAGGCTGTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGCCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGG  >  NC_000913/663239‑663527
                                                                                                                                              |                                                                                                                                                  
ttGATGCCGTCGACATTCAGGCTGTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATAtct                                                                                                                                             <  1:335769/150‑1 (MQ=255)
     gCCGTCGACATTCAGGCTGTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTTGCCGATCAGCGTCATATCTcgcgcg                                                                                                                                       <  1:688835/151‑1 (MQ=255)
     gCCGTCGACATTCAGGCTGTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTcgcgcg                                                                                                                                       <  2:490301/151‑1 (MQ=255)
          cGACATTCAGGCTGTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCt                                                                                                                                  >  1:194502/1‑151 (MQ=255)
            aCATTCAGGCTGTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGt                                                                                                                                >  2:247057/1‑151 (MQ=255)
               ttCAGGCTGTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACt                                                                                                                             >  1:586964/1‑151 (MQ=255)
                       gTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCa                                                                                                                     <  2:164510/151‑1 (MQ=255)
                        ttATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGAc                                                                                                                          <  1:759582/145‑1 (MQ=255)
                        ttATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGAc                                                                                                                          >  2:759639/1‑145 (MQ=255)
                             ccATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATcc                                                                                                               >  1:305670/1‑151 (MQ=255)
                               aTAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATcc                                                                                                               >  1:334378/1‑149 (MQ=255)
                                tAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATcc                                                                                                               >  1:499124/1‑148 (MQ=255)
                                 aaCAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGa                                                                                                            <  2:138423/150‑1 (MQ=255)
                                  aCAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGAcc                                                                                                          >  2:580044/1‑151 (MQ=255)
                                     ggCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATg                                                                                                       >  1:621590/1‑151 (MQ=255)
                                         gTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTAcc                                                                                                   <  2:344878/151‑1 (MQ=255)
                                            aCGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTc                                                                                                >  1:429266/1‑151 (MQ=255)
                                             cGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCt                                                                                               <  2:318374/151‑1 (MQ=255)
                                             cGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCt                                                                                               <  1:65292/151‑1 (MQ=255)
                                                ttCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTgg                                                                                             >  2:336996/1‑150 (MQ=255)
                                                 tCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGa                                                                                            >  2:409305/1‑150 (MQ=255)
                                                  cAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGaa                                                                                           <  2:458064/150‑1 (MQ=255)
                                                   aGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAg                                                                                          >  2:416203/1‑150 (MQ=255)
                                                   aGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGt                                                                                         >  1:661026/1‑151 (MQ=255)
                                                      tGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTggg                                                                                      >  1:740270/1‑151 (MQ=255)
                                                       ggCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGt                                                                                     <  2:653011/151‑1 (MQ=255)
                                                         cGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTAt                                                                                   >  1:836953/1‑151 (MQ=255)
                                                          gAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTAtt                                                                                  >  2:552206/1‑151 (MQ=255)
                                                                cGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTc                                                                              >  2:431172/1‑149 (MQ=255)
                                                                 gTTAAACGGAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTAACGTCTGGAAGTGGGTATTTTTCAgg                                                                           >  1:297929/1‑151 (MQ=255)
                                                                  ttAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGc                                                                          >  1:534600/1‑151 (MQ=255)
                                                                  ttAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGc                                                                          >  1:847067/1‑151 (MQ=255)
                                                                     aaCGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCa                                                                       <  1:21175/151‑1 (MQ=255)
                                                                      aCGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGccc                                                                        >  1:854409/1‑149 (MQ=255)
                                                                       cGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGt                                                                     <  2:814444/151‑1 (MQ=255)
                                                                        gTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAACGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTg                                                                    <  2:371532/151‑1 (MQ=255)
                                                                         tAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGc                                                                   >  1:802122/1‑151 (MQ=255)
                                                                           aaTTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGt                                                                 <  1:125162/151‑1 (MQ=255)
                                                                           aaTTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGt                                                                 <  1:336700/151‑1 (MQ=255)
                                                                           aaTTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGCCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGt                                                                 >  2:664142/1‑151 (MQ=255)
                                                                              tctttttctttATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTa                                                               >  1:6533/1‑150 (MQ=255)
                                                                                 ttttctttATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAAc                                                             <  2:573068/149‑1 (MQ=255)
                                                                                 ttttctttATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACg                                                            <  1:858937/150‑1 (MQ=255)
                                                                                 ttttctttATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGt                                                           >  2:404700/1‑151 (MQ=255)
                                                                                  tttctttATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGt                                                           >  1:329312/1‑150 (MQ=255)
                                                                                              tCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTtcatca                                               >  2:497025/1‑150 (MQ=255)
                                                                                                gAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGTCCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGc                                            >  1:839809/1‑151 (MQ=255)
                                                                                                  gTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGcc                                           >  1:403485/1‑150 (MQ=255)
                                                                                                  gTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGcc                                           >  1:351165/1‑150 (MQ=255)
                                                                                                    attcattcGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCcaac                                        >  1:219335/1‑151 (MQ=255)
                                                                                                        attcGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACaaa                                     >  1:405819/1‑150 (MQ=255)
                                                                                                          tcGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGc                                  >  1:455260/1‑151 (MQ=255)
                                                                                                          tcGGTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGc                                  >  2:84698/1‑151 (MQ=255)
                                                                                                             gTACGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTc                               <  1:492531/151‑1 (MQ=255)
                                                                                                               aCGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTcc                              <  1:208848/150‑1 (MQ=255)
                                                                                                               aCGTCACGGATCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCt                             <  1:448238/151‑1 (MQ=255)
                                                                                                                        aTCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTAcc                      >  2:673069/1‑149 (MQ=255)
                                                                                                                        aTCAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTAcc                      >  2:48089/1‑149 (MQ=255)
                                                                                                                          cAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGc                   <  1:725507/150‑1 (MQ=255)
                                                                                                                          cAATGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCg                  >  1:2710/1‑151 (MQ=255)
                                                                                                                            aTGCCTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGc                >  1:728661/1‑151 (MQ=255)
                                                                                                                               ccTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCaaa             <  2:412321/151‑1 (MQ=255)
                                                                                                                               ccTGGCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCaa              >  1:100987/1‑150 (MQ=255)
                                                                                                                                  ggCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATc          <  1:22723/151‑1 (MQ=255)
                                                                                                                                  ggCCGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATc          <  1:852318/151‑1 (MQ=255)
                                                                                                                                     cGATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGc       >  1:354485/1‑151 (MQ=255)
                                                                                                                                      gATCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGcc      >  2:167763/1‑151 (MQ=255)
                                                                                                                                       aTCAGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCa     <  1:445129/151‑1 (MQ=255)
                                                                                                                                          aGCGTCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATgg  >  1:666894/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TTGATGCCGTCGACATTCAGGCTGTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCATTCGGTACGTCACGGATCAATGCCTGGCCGATCAGCGCCATATCTCGCGCGGAGCTGTACTGACCATCAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGG  >  NC_000913/663239‑663527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: