Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	93,668	A→G	E168G (GAG→GGG)	murE →	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	93,668	0	A	G	100.0%	167.4 / NA	47	E168G (GAG→GGG)	murE	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/26);  total (21/26)

GGCAAAACCACGACTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTG  >  NC_000913/93520‑93814                                                                                                                                                     |                                                                                                                                                   ggCAAAACCACGACTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGc                                                                                                                                                  >  2:243001/1‑151 (MQ=255)    aaaaCCACGACTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTgg                                                                                                                                               <  1:286816/151‑1 (MQ=255)      aaCCACGACTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCg                                                                                                                                             >  1:38489/1‑151 (MQ=255)            gACTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCgggg                                                                                                                                          >  2:745257/1‑148 (MQ=255)                aCCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCtggtg                                                                                                                                    >  2:517859/1‑150 (MQ=255)                aCCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCtggt                                                                                                                                     <  1:52772/149‑1 (MQ=255)                 cccAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCtggtgg                                                                                                                                   >  1:845755/1‑150 (MQ=255)                   cAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATc                                                                                                                                <  2:338900/151‑1 (MQ=255)                        gTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGgc                                                                                                                           <  1:164971/151‑1 (MQ=255)                              gcAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACg                                                                                                                     <  1:542123/151‑1 (MQ=255)                                   ggAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGtttt                                                                                                                 <  2:258216/150‑1 (MQ=255)                                             gCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGATTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGa                                                                                                       <  2:385637/150‑1 (MQ=255)                                                tGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAg                                                                                                     <  1:753023/149‑1 (MQ=255)                                                    gAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTtcct                                                                                               <  2:738104/151‑1 (MQ=255)                                                                   aTGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTAc                                                                                <  1:208905/151‑1 (MQ=255)                                                                    tGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTAc                                                                                >  1:563633/1‑150 (MQ=255)                                                                     gggCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTggga                                                                                 >  2:836583/1‑146 (MQ=255)                                                                     gggCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACATTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACa                                                                               <  1:839726/150‑1 (MQ=255)                                                                                     ggCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGATTTGGGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTggcgg                                                                 >  2:12878/1‑148 (MQ=255)                                                                                       ccTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGGAGTTTCCTCCCACGGGCTGGGACAGCACCGTGTGGCGGCAtt                                                             >  2:484136/1‑150 (MQ=255)                                                                                          gctgGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGGGGATCAGGGGGCGACGGTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGGACAGCACCGTGTGGCGGCATTgga                                                          >  1:70133/1‑148 (MQ=255)                                                                                               ggAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTgc                                                    <  2:120441/151‑1 (MQ=255)                                                                                                  aaGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTgcggc                                                 <  2:458371/151‑1 (MQ=255)                                                                                                     tGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGGACAGCACCGTGTGGCGGCATTGAAATTTgcgg                                                  >  2:803438/1‑147 (MQ=255)                                                                                                      gATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTc                                              <  2:70156/150‑1 (MQ=255)                                                                                                      gATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTc                                              <  2:667574/150‑1 (MQ=255)                                                                                                      gATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCg                                             <  1:43522/151‑1 (MQ=255)                                                                                                      gATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCAGGGGGATTAGGGCGGGACGTTTTGCGCAATTGAAGGTTCCTCCCACGGGGTTGGGCAGGACCGGGGGGCGGCCGTGGAAGTTGAGgcggcg                                             >  1:122101/1‑151 (MQ=255)                                                                                                            gACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGGGGATCAGGGCGCGACGTTTTTCGCAATAGGAGTTTCCTCCCACGGGCCGGTACAGCACCGTGTGGCGGCGTTGAAACTTTCCGCGTCGGTCtt                                        >  2:99427/1‑150 (MQ=255)                                                                                                               aGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGGGGATCAGGGCGCGACGTTTTTCGCAATGGGAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGGCTTTAcc                                    >  1:400329/1‑151 (MQ=255)                                                                                                                     tACAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGAAAAGGACCGGGTGGCGGGATTGAAATTTTCGGCGTCGGGCTTTACCAACtt                               >  2:210534/1‑150 (MQ=255)                                                                                                                      aCAACCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGGTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACtt                               >  1:54636/1‑149 (MQ=255)                                                                                                                        aaCCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGc                           <  1:168548/151‑1 (MQ=255)                                                                                                                         aCCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGcc                          >  2:216354/1‑151 (MQ=255)                                                                                                                         aCCGGTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGcc                          <  2:746962/151‑1 (MQ=255)                                                                                                                            ggTTCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCcgcg                       <  1:484100/151‑1 (MQ=255)                                                                                                                               tCGGCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGAt                     <  2:675384/150‑1 (MQ=255)                                                                                                                                 ggCAGTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCAc                  <  1:247989/151‑1 (MQ=255)                                                                                                                                     gTCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTg              <  1:572736/151‑1 (MQ=255)                                                                                                                                      tCGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGGCTTTACCAACTTAAGCCGCGATCACCtt               >  2:845446/1‑149 (MQ=255)                                                                                                                                       cGATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGAt            <  1:291652/151‑1 (MQ=255)                                                                                                                                        gATGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGAtt           >  2:499793/1‑151 (MQ=255)                                                                                                                                         aTGTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTa          <  2:38511/151‑1 (MQ=255)                                                                                                                                           gTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTAt         <  1:338443/150‑1 (MQ=255)                                                                                                                                           gTTCAGCATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGGACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTAt         >  1:511737/1‑150 (MQ=255)                                                                                                                                                gCATGGGCTGGCGGGGCTGGGGGATCAGGGCGCGACACTTTGCGCAATGGGAGTTTCCTCCCACGGGCTGGTAGAGGAACGTGTGGCGGCATTGAAAATTGCGGCGTCGGTGTTTACCAACTTAAGCCGGGCTCACCCTGATTATCATgg    >  1:574167/1‑150 (MQ=255)                                                                                                                                                 cATGGGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTg  <  2:708331/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GGCAAAACCACGACTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTTCCTCCCACGGGCTGGTACAGCACCGTGTGGCGGCATTGAAATTTGCGGCGTCGGTCTTTACCAACTTAAGCCGCGATCACCTTGATTATCATGGTG  >  NC_000913/93520‑93814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑