Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	729,072	A→G	intergenic (‑251/‑62)	kdpF ← / → ybfA	potassium ion accessory transporter subunit/DUF2517 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	729,072	0	A	G	100.0%	220.9 / NA	58	intergenic (‑251/‑62)	kdpF/ybfA	potassium ion accessory transporter subunit/DUF2517 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/31);  total (27/31)

AAAAGCCATGATTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCGTTCTGGCGCTGC  >  NC_000913/728927‑729215                                                                                                                                                  |                                                                                                                                                aaaaGCCATGATTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATaa                                                                                                                                            <  2:725629/151‑1 (MQ=255) aaaaGCCATGATTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATaa                                                                                                                                            <  1:185612/151‑1 (MQ=255) aaaaGCCATGATTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATaa                                                                                                                                            <  2:356543/151‑1 (MQ=255) aaaaGCCATGATTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATa                                                                                                                                             >  2:106625/1‑150 (MQ=255)  aaaGCCATGATTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATGAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGAGGTACTATTTTCATCGGATa                                                                                                                                             >  1:179394/1‑149 (MQ=255)  aaaGCCATGATTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATaaa                                                                                                                                           <  2:398871/151‑1 (MQ=255)    aGCCATGATTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAAc                                                                                                                                          >  2:257303/1‑150 (MQ=255)    aGCCATGATTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACa                                                                                                                                         <  2:603027/151‑1 (MQ=255)           aTTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCAt                                                                                                                                  >  2:8551/1‑151 (MQ=255)                 cTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTcc                                                                                                                            >  2:441093/1‑151 (MQ=255)                      gTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGc                                                                                                                       >  2:618513/1‑151 (MQ=255)                          aGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCAtt                                                                                                                   <  1:775278/151‑1 (MQ=255)                          aGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCAtt                                                                                                                   <  2:319327/151‑1 (MQ=255)                           gTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTc                                                                                                                  >  1:305141/1‑151 (MQ=255)                               tAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCAcc                                                                                                               <  1:238240/150‑1 (MQ=255)                                 aTTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCggg                                                                                                            >  2:266826/1‑151 (MQ=255)                                     aaCTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGt                                                                                                           >  2:177210/1‑148 (MQ=255)                                       cTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGAt                                                                                                       >  1:331546/1‑150 (MQ=255)                                         tttGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATa                                                                                                      <  2:737003/149‑1 (MQ=255)                                         tttGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATa                                                                                                      <  2:176308/149‑1 (MQ=255)                                            gtgtAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACaaa                                                                                                  <  1:685209/150‑1 (MQ=255)                                                 aCTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAggggg                                                                                            >  2:660128/1‑151 (MQ=255)                                                 aCTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAggggg                                                                                            >  1:179868/1‑151 (MQ=255)                                                    tAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAggggg                                                                                            >  2:153315/1‑148 (MQ=255)                                                    tAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGga                                                                                           <  1:157565/149‑1 (MQ=255)                                                    tAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGga                                                                                           <  2:517200/149‑1 (MQ=255)                                                      aTTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGgaga                                                                                         >  1:721405/1‑149 (MQ=255)                                                         aCAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGt                                                                                    <  1:157840/151‑1 (MQ=255)                                                         aCAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGt                                                                                    >  2:548650/1‑151 (MQ=255)                                                            ggATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATg                                                                                 >  1:844887/1‑151 (MQ=255)                                                              aTGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGa                                                                               <  1:593875/151‑1 (MQ=255)                                                                gAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAAc                                                                             >  1:169942/1‑151 (MQ=255)                                                                 aaTGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAAct                                                                            >  2:737485/1‑151 (MQ=255)                                                                  aTGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAActc                                                                           <  1:611587/151‑1 (MQ=255)                                                                       aataaaCCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTAc                                                                        >  2:721934/1‑149 (MQ=255)                                                                              cATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAatat                                                               >  1:147989/1‑151 (MQ=255)                                                                              cATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAatat                                                               <  2:55315/151‑1 (MQ=255)                                                                                 cAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCt                                                            >  1:151735/1‑151 (MQ=255)                                                                                 cAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCt                                                            <  2:400722/151‑1 (MQ=255)                                                                                     aaGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCAt                                                        <  2:721448/151‑1 (MQ=255)                                                                                     aaGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCAt                                                        <  1:575885/151‑1 (MQ=255)                                                                                        cAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGc                                                     >  1:152623/1‑151 (MQ=255)                                                                                          aaaaTAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCtt                                                   <  1:491398/151‑1 (MQ=255)                                                                                           aaaTAAGTGGTCGGATGAGTAGTAAAATTACACAAATGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGCAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCtt                                                   >  1:571951/1‑150 (MQ=255)                                                                                                    gTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTAc                                         >  1:392151/1‑151 (MQ=255)                                                                                                       ggATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGcc                                      <  2:630336/151‑1 (MQ=255)                                                                                                         aTGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGt                                    <  1:329708/151‑1 (MQ=255)                                                                                                           gAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTAc                                  <  1:106602/151‑1 (MQ=255)                                                                                                               agtaAAATTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTAt                              <  2:262343/151‑1 (MQ=255)                                                                                                                   aaaTTACACAAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCgg                          >  2:306181/1‑151 (MQ=255)                                                                                                                           caAAAGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCg                  <  2:4917/151‑1 (MQ=255)                                                                                                                               aGGCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCg              <  2:150138/151‑1 (MQ=255)                                                                                                                                 gCGGTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCGtt            <  2:651816/151‑1 (MQ=255)                                                                                                                                   ggTACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCGTTCt          <  1:406914/151‑1 (MQ=255)                                                                                                                                     tACTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCGTTCTgg        >  1:754008/1‑151 (MQ=255)                                                                                                                                      aCTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCGTTCTGgc       <  2:829947/151‑1 (MQ=255)                                                                                                                                       cTATTTTCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCGTTCTGgc       <  2:610943/150‑1 (MQ=255)                                                                                                                                           tttCATCGGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCGTTCTGGCGCTGc  >  2:464726/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                AAAAGCCATGATTTAACTAATGTTTAGTATTAATTTAACTTTTGTGTAACTTAATTACAGGATGAATGTAAATAAACCATCAATAAGCAAAAATAAGTGGTCGGATGAGTAGTAAAATTACACAAAAGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCGTTCTGGCGCTGC  >  NC_000913/728927‑729215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑