Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 766,588 C→T A202V (GCT→GTT)  mngA → fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913766,5880CT98.1% 171.6 / ‑4.1 54A202V (GCT→GTT) mngAfused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (23/29);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

GCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAA  >  NC_000913/766440‑766724
                                                                                                                                                    |                                                                                                                                        
gcgcGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCATCGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTg                                                                                                                                        <  1:546152/151‑1 (MQ=255)
   cgTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCgg                                                                                                                                     >  1:724280/1‑151 (MQ=255)
    gTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGa                                                                                                                                    <  1:632372/151‑1 (MQ=255)
        aCAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAatat                                                                                                                                >  2:128696/1‑151 (MQ=255)
        aCAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAatat                                                                                                                                <  2:690341/151‑1 (MQ=255)
        aCAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAata                                                                                                                                 <  2:446798/150‑1 (MQ=255)
          aGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCt                                                                                                                              >  2:795331/1‑151 (MQ=255)
              ggCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGa                                                                                                                           >  2:376064/1‑150 (MQ=255)
                  acgacgCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAg                                                                                                                      <  2:531003/151‑1 (MQ=255)
                    gacgCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGc                                                                                                                     <  2:629182/150‑1 (MQ=255)
                     acgCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCg                                                                                                                   <  1:782653/151‑1 (MQ=255)
                     acgCCTGATGAGTTGCTATCGGCGCTGGATGACAAGGGAGGAACGCAACATTCTTCCTCTTTTTCCAACGCGGCAACTATCGTCTGTGTAACGGCCTGTCCGGCGGCTATTGCTCACACCTATTTTGTTGCGGAATATCTGGaaaa                                                                                                                        >  1:555437/1‑146 (MQ=255)
                       gCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGATGCGGAATATCTGGAAAAAGCCgg                                                                                                                  >  2:697068/1‑150 (MQ=255)
                            atgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCaa                                                                                                            <  1:104700/151‑1 (MQ=255)
                                gTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTc                                                                                                        <  1:617158/151‑1 (MQ=255)
                                 ttGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTc                                                                                                        <  2:694374/150‑1 (MQ=255)
                                 ttGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCg                                                                                                       >  2:297546/1‑151 (MQ=255)
                                 ttGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCg                                                                                                       >  1:761540/1‑151 (MQ=255)
                                         ggCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAAt                                                                                               <  2:64049/151‑1 (MQ=255)
                                            gcTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGtt                                                                                            <  2:173339/151‑1 (MQ=255)
                                                    aCAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGa                                                                                    <  1:855254/151‑1 (MQ=255)
                                                        gggAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGaaaa                                                                                 >  2:54488/1‑150 (MQ=255)
                                                         ggAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTTTATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAAc                                                                               <  2:423303/151‑1 (MQ=255)
                                                             gCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAgg                                                                           <  2:702281/151‑1 (MQ=255)
                                                                 gCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCt                                                                       <  1:535490/151‑1 (MQ=255)
                                                                        tCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACgg                                                                  <  2:673963/149‑1 (MQ=255)
                                                                           gCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCa                                                                <  2:493258/148‑1 (MQ=255)
                                                                           gCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCAtt                                                              <  2:505188/150‑1 (MQ=255)
                                                                           gCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCAtt                                                              <  2:504247/150‑1 (MQ=255)
                                                                            ccTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGACATTGa                                                            <  1:348792/151‑1 (MQ=255)
                                                                            ccTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGGATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTg                                                             >  2:656450/1‑150 (MQ=255)
                                                                              tctTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTg                                                             >  1:28274/1‑148 (MQ=255)
                                                                              tctTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTg                                                             >  1:323262/1‑148 (MQ=255)
                                                                                 ttttCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAggg                                                        >  2:355894/1‑150 (MQ=255)
                                                                                     ccAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGttt                                                   <  2:160302/151‑1 (MQ=255)
                                                                                      cAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGttt                                                   >  1:563811/1‑150 (MQ=255)
                                                                                      cAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTa                                                  <  2:711359/151‑1 (MQ=255)
                                                                                       aaCGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTa                                                  >  2:405064/1‑150 (MQ=255)
                                                                                           cgcCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAAcggc                                             <  1:795275/151‑1 (MQ=255)
                                                                                               aaCTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAt                                         >  2:606350/1‑151 (MQ=255)
                                                                                                aCTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAt                                         >  2:49435/1‑150 (MQ=255)
                                                                                                  tATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAtta                                       >  1:455436/1‑148 (MQ=255)
                                                                                                  tATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATc                                        >  2:302159/1‑149 (MQ=255)
                                                                                                   aTCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCa                                       <  2:161640/149‑1 (MQ=255)
                                                                                                     cGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAAc                                     >  2:282099/1‑149 (MQ=255)
                                                                                                     cGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTc                                   <  1:324921/151‑1 (MQ=255)
                                                                                                       tCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGGTCAACTCa                                  >  1:663974/1‑150 (MQ=255)
                                                                                                             tAACGGCCTGTCCGGCGGGGATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTAGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAAAGGCGGATCaaaacacta                               >  1:83680/1‑140 (MQ=255)
                                                                                                                 ggCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAAcc                       <  1:518407/151‑1 (MQ=255)
                                                                                                                 ggCCTGTCCGGCGGGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAAcc                       <  2:243923/151‑1 (MQ=255)
                                                                                                                          ggcggGTATTGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAAATCAATAGTGCAACCGCCCGGAtt              >  1:417073/1‑151 (MQ=255)
                                                                                                                                   tGCTCACACCTATATGGTTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAAAAGTGCAACCGCCTGTATTTTTTCgg       >  1:568629/1‑149 (MQ=255)
                                                                                                                                     atCACACCTATATGGCTGCGGAAGATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATTAAATCAAAAGAGCAAAAGCCTGTATTTTTGCGGCt     >  1:97818/2‑149 (MQ=255)
                                                                                                                                       caccCCTATAGGTTTGCGAAATATCGGGAAAAAGCCGGACGCAAGCTGGGGATAAATGTTTACTTTGAAAAACAGGGCGCTACGGCCATTGAAGTGCTTTAAACGGCGGATCAACACAATAGTGCAACCGCCTGTATTTTTGCGGCTGaa  <  1:186637/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
GCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAA  >  NC_000913/766440‑766724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑