Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	768,591	T→C	V205A (GTG→GCG)	mngB →	alpha‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	768,591	0	T	C	98.8%	276.4 / ‑4.3	80	V205A (GTG→GCG)	mngB	alpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (41/38);  total (42/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CACGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGTGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCTTTGAAGAGG  >  NC_000913/768443‑768737                                                                                                                                                     |                                                                                                                                                   cACGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACgcgc                                                                                                                                                  >  1:524066/1‑151 (MQ=255)   cGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTg                                                                                                                                                >  2:81335/1‑151 (MQ=255)   cGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTg                                                                                                                                                >  1:730880/1‑151 (MQ=255)    ggTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTgg                                                                                                                                               >  1:515790/1‑151 (MQ=255)       aCTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGaaa                                                                                                                                            <  2:233110/151‑1 (MQ=255)          gATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGaaaa                                                                                                                                           >  2:151060/1‑149 (MQ=255)            tAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGAGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCg                                                                                                                                       >  1:26432/1‑151 (MQ=255)              aaaCCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTTTTTTGACGCGCTGGAAAAAGCGTc                                                                                                                                     >  2:644256/1‑151 (MQ=255)               aaCCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCt                                                                                                                                    >  2:291910/1‑151 (MQ=255)                   gAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTaa                                                                                                                                <  2:698730/151‑1 (MQ=255)                    aGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAAc                                                                                                                               >  2:689621/1‑151 (MQ=255)                    aGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAAc                                                                                                                               <  2:264117/151‑1 (MQ=255)                     gTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAAcc                                                                                                                              <  1:1635/151‑1 (MQ=255)                      tttttGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAAcc                                                                                                                              >  2:92648/1‑150 (MQ=255)                        tttGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCaa                                                                                                                            <  2:539370/150‑1 (MQ=255)                         ttGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCaa                                                                                                                            >  2:394818/1‑149 (MQ=255)                                 aaGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTtg                                                                                                                  <  2:676452/151‑1 (MQ=255)                                    cAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTtgctg                                                                                                               <  1:537498/151‑1 (MQ=255)                                     ggTGACGGTAGCGGCGTGACGGGGAAGGAGGGGCCGCTGCGCTACGCCATCGGTAAGTACTTACGTGCCGACGAAAAAGGAATACGTAAACGCCTCGACAGTTATTTTGACGTGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTtgct                                                                                                                >  1:193944/2‑149 (MQ=255)                                     aGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTtgctgc                                                                                                              <  2:488960/151‑1 (MQ=255)                                          cGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAAt                                                                                                         <  1:74727/151‑1 (MQ=255)                                            gTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATgg                                                                                                       <  2:273093/151‑1 (MQ=255)                                              aGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGc                                                                                                     >  1:507033/1‑151 (MQ=255)                                              aGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGc                                                                                                     <  2:315088/151‑1 (MQ=255)                                               gCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCa                                                                                                    <  2:688591/151‑1 (MQ=255)                                                 gAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAACAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCAt                                                                                                   <  1:360610/150‑1 (MQ=255)                                                   aGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGAc                                                                                                >  2:247419/1‑151 (MQ=255)                                                      gACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAg                                                                                             <  2:524104/151‑1 (MQ=255)                                                          gcgcAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGc                                                                                         >  2:635354/1‑151 (MQ=255)                                                            gcAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGTTGCCGAATGGGCATGACCAGATGCCa                                                                                       <  1:836624/151‑1 (MQ=255)                                                              aGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCAtt                                                                                     >  1:154583/1‑151 (MQ=255)                                                              aGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCAtt                                                                                     >  2:111800/1‑151 (MQ=255)                                                              aGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCAtt                                                                                     <  2:570758/151‑1 (MQ=255)                                                                gTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTgc                                                                                   >  2:485688/1‑151 (MQ=255)                                                                gTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTgc                                                                                   >  1:124634/1‑151 (MQ=255)                                                                  gctgcCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTgca                                                                                  <  1:25560/150‑1 (MQ=255)                                                                  gctgcCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTgca                                                                                  <  1:557478/150‑1 (MQ=255)                                                                     gcCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTgcagca                                                                               <  2:342245/150‑1 (MQ=255)                                                                         cTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAat                                                                           >  2:755000/1‑150 (MQ=255)                                                                           gggCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAatat                                                                         <  1:581299/150‑1 (MQ=255)                                                                                  gCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGa                                                                   >  2:677775/1‑149 (MQ=255)                                                                                  gCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGa                                                                   >  1:776022/1‑149 (MQ=255)                                                                                       cGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGAt                                                             <  1:173268/150‑1 (MQ=255)                                                                                          tAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGAt                                                         >  1:563896/1‑151 (MQ=255)                                                                                           aaGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGAt                                                         <  1:66848/150‑1 (MQ=255)                                                                                           aaGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGAt                                                         >  2:432509/1‑150 (MQ=255)                                                                                            aGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATa                                                        >  1:20036/1‑150 (MQ=255)                                                                                               aCTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCt                                                    <  2:19032/151‑1 (MQ=255)                                                                                                  tACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTa                                                   <  2:638026/149‑1 (MQ=255)                                                                                                  tACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTAc                                                  <  2:784279/150‑1 (MQ=255)                                                                                                   aCCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACg                                                 >  1:424329/1‑150 (MQ=255)                                                                                                   aCCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGt                                                <  1:55667/151‑1 (MQ=255)                                                                                                    ccTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTg                                               <  2:849584/151‑1 (MQ=255)                                                                                                    ccTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTg                                               <  1:178897/151‑1 (MQ=255)                                                                                                       gCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTg                                               >  1:170348/1‑148 (MQ=255)                                                                                                         cgacgaAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATc                                          <  2:14107/151‑1 (MQ=255)                                                                                                              aaaaCGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTAccc                                     >  2:12612/1‑151 (MQ=255)                                                                                                                aaCGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTc                                   <  2:763060/151‑1 (MQ=255)                                                                                                                 aCGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCa                                  <  2:785722/151‑1 (MQ=255)                                                                                                                   ggATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAAc                                >  2:533502/1‑151 (MQ=255)                                                                                                                       tACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTa                             >  1:138446/1‑150 (MQ=255)                                                                                                                       tACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTa                             >  2:709109/1‑150 (MQ=255)                                                                                                                       tACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTa                             >  1:207711/1‑150 (MQ=255)                                                                                                                        aCGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAg                           <  2:791887/151‑1 (MQ=255)                                                                                                                         cGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGt                          >  2:247525/1‑151 (MQ=255)                                                                                                                          gTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAGGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGtt                         >  2:189889/1‑151 (MQ=255)                                                                                                                          gTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGtt                         >  1:102358/1‑151 (MQ=255)                                                                                                                           tAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGttt                        >  2:345861/1‑151 (MQ=255)                                                                                                                           tAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGttt                        >  2:292695/1‑151 (MQ=255)                                                                                                                           tAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGttt                        >  2:820387/1‑151 (MQ=255)                                                                                                                            aaaCGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAGCAAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCTATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTtg                       >  1:566203/1‑151 (MQ=255)                                                                                                                                 ccTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGtgatg                  <  1:151037/151‑1 (MQ=255)                                                                                                                                    cGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAg                <  2:698876/150‑1 (MQ=255)                                                                                                                                    cGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGc               <  2:332736/151‑1 (MQ=255)                                                                                                                                     gACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGcc              >  2:443351/1‑151 (MQ=255)                                                                                                                                      aCAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCg             >  2:170697/1‑151 (MQ=255)                                                                                                                                       cAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGc            >  1:538851/1‑151 (MQ=255)                                                                                                                                       cAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGc            >  2:811940/1‑151 (MQ=255)                                                                                                                                        aGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCt           <  2:299333/151‑1 (MQ=255)                                                                                                                                              tttGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCTTTGaa      <  2:442232/150‑1 (MQ=255)                                                                                                                                                 gACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCTTTGAAGAgg  <  2:432522/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CACGGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGTGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCTTTGAAGAGG  >  NC_000913/768443‑768737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑