Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	776,165	T→G	V106G (GTC→GGC)	tolR →	membrane spanning protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	776,165	0	T	G	100.0%	228.6 / NA	63	V106G (GTC→GGC)	tolR	membrane spanning protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/40);  total (23/40)

AGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGC  >  NC_000913/776017‑776308                                                                                                                                                     |                                                                                                                                                aGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCt                                                                                                                                               >  2:491930/1‑151 (MQ=255)  gCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCtt                                                                                                                                              >  2:442092/1‑151 (MQ=255)    aGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTc                                                                                                                                            <  1:750767/151‑1 (MQ=255)       aaCGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTg                                                                                                                                          <  1:482652/150‑1 (MQ=255)        aCGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGAt                                                                                                                                        <  1:644005/151‑1 (MQ=255)           aTAATCCGCCAGTGATTGTTGAAGAGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCg                                                                                                                                      >  1:612229/1‑150 (MQ=255)             aaTCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTg                                                                                                                                   <  1:311731/151‑1 (MQ=255)             aaTCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTg                                                                                                                                   >  1:244676/1‑151 (MQ=255)                   ccAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCa                                                                                                                              >  2:842916/1‑150 (MQ=255)                     aGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCaaa                                                                                                                            >  2:599214/1‑150 (MQ=255)                      gTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCaaa                                                                                                                            >  2:596828/1‑149 (MQ=255)                         aTTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGAtg                                                                                                                       >  1:818202/1‑151 (MQ=255)                             ttgAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGGAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGc                                                                                                                    <  2:190484/150‑1 (MQ=255)                              tgAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCt                                                                                                                  >  1:809576/1‑151 (MQ=255)                                 aGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTAc                                                                                                               >  1:310875/1‑151 (MQ=255)                                 aGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTAc                                                                                                               <  2:406668/151‑1 (MQ=255)                                    gtCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAt                                                                                                            <  1:815876/151‑1 (MQ=255)                                    gtCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAt                                                                                                            <  2:601078/151‑1 (MQ=255)                                    gtCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCCGATCGGTGGCGCAAAAGATGTGCCTTACGAt                                                                                                            <  1:117405/151‑1 (MQ=255)                                    gtCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCCTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAt                                                                                                            >  2:639350/1‑151 (MQ=255)                                        ggTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaaa                                                                                                        <  2:330853/151‑1 (MQ=255)                                        ggTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaa                                                                                                         <  2:67723/150‑1 (MQ=255)                                        ggTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaa                                                                                                         <  2:10132/150‑1 (MQ=255)                                              ggTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAAtt                                                                                                   <  1:615187/150‑1 (MQ=255)                                               gTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTaa                                                                                                 <  2:92718/151‑1 (MQ=255)                                                      cacCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTg                                                                                          <  2:557921/151‑1 (MQ=255)                                                      cacCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTg                                                                                          >  1:387238/1‑151 (MQ=255)                                                        ccGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTg                                                                                          <  1:387073/149‑1 (MQ=255)                                                        ccGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGa                                                                                         <  2:353239/150‑1 (MQ=255)                                                            ggtggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACtt                                                                                     <  2:512448/150‑1 (MQ=255)                                                            ggtggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTg                                                                                    >  2:235433/1‑151 (MQ=255)                                                             gtggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGt                                                                                   <  2:571731/151‑1 (MQ=255)                                                                   gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAt                                                                              <  1:410606/150‑1 (MQ=255)                                                                      aaaGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCATCCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTCCGATGAAATAATTAAAGCGCTGAACTTGTTACATAGTg                                                                          <  2:727681/151‑1 (MQ=255)                                                                            cGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGgt                                                                     <  2:1790/150‑1 (MQ=255)                                                                              ccTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGtgtg                                                                  <  1:463902/151‑1 (MQ=255)                                                                              ccTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGtgtg                                                                  >  1:698370/1‑151 (MQ=255)                                                                                    gCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCg                                                            >  2:342719/1‑151 (MQ=255)                                                                                         tACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCggttg                                                        <  1:858566/150‑1 (MQ=255)                                                                                          accaccAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCTAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCggttggt                                                      <  2:672303/151‑1 (MQ=255)                                                                                          accaccAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCggttgg                                                       <  1:394112/150‑1 (MQ=255)                                                                                             accaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTa                                                   <  2:93028/151‑1 (MQ=255)                                                                                                  agCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGAc                                              >  2:345690/1‑151 (MQ=255)                                                                                                      ggtggtGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAg                                          <  1:58885/151‑1 (MQ=255)                                                                                                        tggtggCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGACGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGcc                                        >  2:718825/1‑151 (MQ=255)                                                                                                         ggtggCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGcc                                        <  1:508703/150‑1 (MQ=255)                                                                                                          gtggCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTa                                      <  2:495443/151‑1 (MQ=255)                                                                                                              cGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTa                                  <  1:116399/151‑1 (MQ=255)                                                                                                              cGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTa                                  <  1:577517/151‑1 (MQ=255)                                                                                                                gAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTaa                                 >  1:147270/1‑150 (MQ=255)                                                                                                                            cGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTc                    >  2:602580/1‑151 (MQ=255)                                                                                                                            cGTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTc                    <  1:266956/151‑1 (MQ=255)                                                                                                                             gTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTcc                   <  1:189600/151‑1 (MQ=255)                                                                                                                             gTTTCAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTcc                   <  2:312691/151‑1 (MQ=255)                                                                                                                                 cAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCctt                <  2:747796/150‑1 (MQ=255)                                                                                                                                 cAAGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCATTACGATGAAATAATTAAAGCACTGAACTTGTTCCATAGTGCGGGTGTTAAACCGGTTGGTTTAATGACGCAGCCTAGCTAAACATCTGCGTTTCCctt                <  1:280009/150‑1 (MQ=255)                                                                                                                                   aGGCCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCcttgct             <  2:217050/151‑1 (MQ=255)                                                                                                                                       cAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTTCATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGaa         <  2:822916/151‑1 (MQ=255)                                                                                                                                        aaCCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGaaa        <  1:734980/151‑1 (MQ=255)                                                                                                                                             gAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAagag     >  1:403059/1‑149 (MQ=255)                                                                                                                                             gAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAagag     >  2:837681/1‑149 (MQ=255)                                                                                                                                             gAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAagag     >  2:245392/1‑149 (MQ=255)                                                                                                                                              aaaaCGGGCTTTCTGATCGGTGGCGCAAAAGATGTGACTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGACTATCTAAACATCTGCGTTTCACTTGCTTGAAAGAGAGc  >  1:16083/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                AGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGC  >  NC_000913/776017‑776308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑