Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	97,947	T→C	R287R (CGT→CGC)	murD →	UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	97,947	0	T	C	100.0%	137.6 / NA	40	R287R (CGT→CGC)	murD	UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/23);  total (17/23)

ATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTGCA  >  NC_000913/97808‑98090                                                                                                                                            |                                                                                                                                                atcatcAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCt                                                                                                                                      >  1:571656/1‑151 (MQ=255)   catcaGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGa                                                                                                                                    <  1:43683/151‑1 (MQ=255)       agcagGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGCTGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAg                                                                                                                                 >  1:421467/1‑150 (MQ=255)       agcagGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGc                                                                                                                                <  2:824118/151‑1 (MQ=255)       agcagGGCGAAACCTGGCTGCGGGTTAAAGCCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCCGGCAGATGCTGCTGGGTTACCGCGCGCCCGCGGCCTGaaa                                                                                                                                  >  1:305439/1‑149 (MQ=255)             gCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAAc                                                                                                                          >  1:368099/1‑151 (MQ=255)                aaaCCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACcac                                                                                                                       >  2:118396/1‑151 (MQ=255)                       gCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACt                                                                                                                <  2:444176/151‑1 (MQ=255)                           cGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTc                                                                                                            >  2:842340/1‑151 (MQ=255)                                  aaGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGACAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCa                                                                                                     <  1:87121/151‑1 (MQ=255)                                          aaagctttGAATGCGAAAGAGACGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGAGGCGCTGGCGCCGGCAGATGCTGCCGGGTTACCGCGCGCCCGCAGCCTGAAAGCGTTAACCACATTCACTGGCCTTTCGCATCGCgtt                                                                                              >  1:835730/8‑150 (MQ=255)                                               gCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGtt                                                                                        <  2:46889/151‑1 (MQ=255)                                               gCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGtt                                                                                        <  1:85649/151‑1 (MQ=255)                                               gCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGtt                                                                                        >  2:539630/1‑151 (MQ=255)                                                cTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTtg                                                                                       >  1:682904/1‑151 (MQ=255)                                                    aTGTTAAAGAGATGAAACTTTCCGGGCAGCATAACTACGCCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCTGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCt                                                                                   <  2:659297/151‑1 (MQ=255)                                                        gAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAg                                                                               <  1:842282/151‑1 (MQ=255)                                                          aaGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCa                                                                             <  2:602571/151‑1 (MQ=255)                                                           agagATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCAt                                                                            <  1:804921/151‑1 (MQ=255)                                                                tGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACgg                                                                       >  2:537091/1‑151 (MQ=255)                                                                    aCTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTa                                                                   <  1:34514/151‑1 (MQ=255)                                                                        tCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGt                                                                <  1:676462/150‑1 (MQ=255)                                                                         ccGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGtt                                                               <  2:413883/150‑1 (MQ=255)                                                                             gcagcaTAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGAtt                                                          >  1:460284/1‑151 (MQ=255)                                                                                  aTAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGa                                                     <  2:721026/151‑1 (MQ=255)                                                                                    aaCTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGAt                                                    <  1:242074/150‑1 (MQ=255)                                                                                       tACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCg                                                 >  2:51736/1‑150 (MQ=255)                                                                                         cacCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGaa                                               >  2:240179/1‑150 (MQ=255)                                                                                           ccAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGc                                            <  1:438889/151‑1 (MQ=255)                                                                                             aaTGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGa                                          <  2:796606/151‑1 (MQ=255)                                                                                                gcgctggcggcgctggcgCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGAcc                                        >  2:55665/1‑150 (MQ=255)                                                                                                 cgctggcggcgctggcgCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCaa                                      <  2:652838/151‑1 (MQ=255)                                                                                                    tggcggcgctggcgCTGGCAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGt                                   >  2:23971/1‑151 (MQ=255)                                                                                                               gcgctggcAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCTCTTTGAAGTTGTGCTGGAGCATAACGGCGTTCGTTGGAGTAACGATTCGAAAGCGACCAACGTCGGCAGTAc                          <  1:257610/149‑1 (MQ=255)                                                                                                                    ggcAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAg                     <  2:292492/149‑1 (MQ=255)                                                                                                                    ggcAGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAg                     <  2:108926/149‑1 (MQ=255)                                                                                                                       aGATGCTGCAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAgcggcg                >  2:261189/1‑151 (MQ=255)                                                                                                                               cAGGGTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGa            >  1:476559/1‑147 (MQ=255)                                                                                                                                   gTTACCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTg    <  2:2893/151‑1 (MQ=255)                                                                                                                                      aCCGCGCGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTGCa  <  1:297376/150‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                ATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACCACATTCACTGGTCTGCCGCATCGCTTTGAAGTTGTGCTGGAGCATAACGGCGTACGTTGGATTAACGATTCGAAAGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTGCA  >  NC_000913/97808‑98090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑